%0 Journal Article %A Eide, Truls %A Marsden, Howard S %A Leib, David A %A Cunningham, Charles %A Davison, Andrew J %A Langeland, Nina %A Haarr, Lars %T Identification of the UL4 protein of herpes simplex virus type 1. %D 1998 %J Journal of General Virology, %V 79 %N 12 %P 3033-3038 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-79-12-3033 %I Microbiology Society, %X Herpes simplex virus type 1 gene UL4 is predicted to encode a 199-amino-acid protein with a molecular mass of 21·5 kDa. We report here identification of this protein and its localization in the nuclei of infected cells. Antisera raised against oligopeptides corresponding to the C terminus of the predicted UL4 protein were used for identification of a 25 kDa protein as the product of the UL4 gene. This protein was not detected in cells infected with a UL4 defective mutant virus, but was synthesized by coupled in vitro transcription-translation of the UL4 gene. Synthesis of the 25 kDa protein was blocked by phosphonoacetic acid, an inhibitor of DNA synthesis, indicating that the UL4 gene is expressed with γ kinetics. Subcellular fractionation showed the protein to be localized in the nucleus. It was not detected in virions or light particles. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-12-3033