@article{mbs:/content/journal/jgv/10.1099/0022-1317-79-12-3033, author = "Eide, Truls and Marsden, Howard S and Leib, David A and Cunningham, Charles and Davison, Andrew J and Langeland, Nina and Haarr, Lars", title = "Identification of the UL4 protein of herpes simplex virus type 1.", journal= "Journal of General Virology", year = "1998", volume = "79", number = "12", pages = "3033-3038", doi = "https://doi.org/10.1099/0022-1317-79-12-3033", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-12-3033", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "Herpes simplex virus type 1 gene UL4 is predicted to encode a 199-amino-acid protein with a molecular mass of 21·5 kDa. We report here identification of this protein and its localization in the nuclei of infected cells. Antisera raised against oligopeptides corresponding to the C terminus of the predicted UL4 protein were used for identification of a 25 kDa protein as the product of the UL4 gene. This protein was not detected in cells infected with a UL4 defective mutant virus, but was synthesized by coupled in vitro transcription-translation of the UL4 gene. Synthesis of the 25 kDa protein was blocked by phosphonoacetic acid, an inhibitor of DNA synthesis, indicating that the UL4 gene is expressed with γ kinetics. Subcellular fractionation showed the protein to be localized in the nucleus. It was not detected in virions or light particles.", }