1887

Abstract

Cytopathogenicity of classical swine fever virus (CSFV) depends on the presence of defective particles containing a subgenomic (sg) RNA with a defined deletion. In a previous report we described the spontaneous generation of this sg RNA and therefore of cytopathogenic (cp) CSFV in porcine kidney cell cultures persistently infected with CSFV. Frequently, some cells survived the CPE and could be further propagated. They remained positive for viral antigen and continued to shed complete virus and in most cases also defective virus particles. SK- 6 cells that had survived the CPE (CPE cells) were used to investigate these findings further. In contrast to persistently infected cells that had not experienced a CPE, CPE cells were protected from the CPE when superinfected with cp CSFV or with cp bovine viral diarrhoea virus. Similarly, cells which were rescued and further propagated after acute infection with cp CSFV also proved to be protected from the CSFV-induced CPE. When either virus obtained from CPE cells that had spontaneously lost the sg RNA or virus from which defective particles had been removed was used to establish persistently infected cells, these cells were also protected from the CPE after superinfection with cp CSFV. These findings suggest that the virus contained in CPE cells confers on the host cell the ability to resist the CSFV-induced CPE. However, when naive cells were infected with supernatants from CPE cells that contained defective virus particles, the CPE reappeared within three to five virus passages, indicating that the sg RNA retained its cytopathogenic potential.

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1998-12-01
2022-01-25
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References

  1. Flores E. F., Donis R. O. 1995; Isolation of a mutant MDBK cell line resistant to bovine viral diarrhea virus infection due to a block in viral entry. Virology 208:565–575
    [Google Scholar]
  2. Hoff H. S., Donis R. O. 1997; Induction of apoptosis and cleavage of poly(ADP-ribose) polymerase by cytopathic bovine viral diarrhea virus infection. Virus Research 49:101–113
    [Google Scholar]
  3. Kasza L., Shadduck J. A., Christofinis G. J. 1972; Establishment, viral susceptibility and biological characteristics of a swine kidney cell line SK-6. Research in Veterinary Science 13:46–51
    [Google Scholar]
  4. Meyers G., Thiel H. J. 1995; Cytopathogenicity of classical swine fever virus caused by defective interfering particles. Journal of Virology 69:3683–3689
    [Google Scholar]
  5. Meyers G., Thiel H. J. 1996; Molecular characterization of pestiviruses. Advances in Virus Research 47:53–118
    [Google Scholar]
  6. Mittelholzer C., Moser C., Tratschin J. D., Hofmann M. A. 1997; Generation of cytopathogenic subgenomic RNA of classical swine fever virus in persistently infected porcine cell lines. Virus Research 51:125–137
    [Google Scholar]
  7. Moser C., Tratschin J. D., Hofmann M. A. 1998; A recombinant classical swine fever virus stably expresses a marker gene. Journal of Virology 72:5318–5322
    [Google Scholar]
  8. Ruggli N., Tratschin J. D., Mittelholzer C., Hofmann M. A. 1996; Nucleotide sequence of classical swine fever virus strain Alfort/187 and transcription of infectious RNA from stably cloned full-length cDNA. Journal of Virology 70:3478–3487
    [Google Scholar]
  9. Thiel H. -J., Plagemann P. G. W., Moennig V. 1996; Pestiviruses. In Fields Virology, 3rd edn. pp 1059–1073 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  10. Wengler G., Bradley D. W., Collett M. S., Heinz F. X., Schlesinger R. W., Strauss J. H. 1995; Family Flaviviridae InVirus Taxonomy. Sixth Report of the International Committee on Taxonomy of Viruses pp 415–427 Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D. Edited by Vienna & New York: Springer-Verlag;
    [Google Scholar]
  11. Zhang G., Aldridge S., Clarke M. C., McCauley J. W. 1996; Cell death induced by cytopathic bovine viral diarrhoea virus is mediated by apoptosis. Journal of General Virology 77:1677–1681
    [Google Scholar]
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