1887

Abstract

One anticipated advantage of DNA immunization is the potential to create multivalent vaccines. We have examined the effects of mixing plasmids into single formulations using plasmids expressing four different membrane bound glycoproteins from bovine herpesvirus-1 (BHV-1), bovine para- influenzavirus-3 (bPI3) and human influenza virus (HINF). Plasmids were delivered by intradermal injection into the tails of mice and the types of responses generated were clearly affected by the expressed antigen. Plasmids expressing glycoproteins B and D of BHV-1, and the haemagglutinin/ neuraminidase of bPI3, generated responses with a predominance of IgG1, suggestive of a Th2 type of response. In contrast, the plasmid expressing HINF haemagglutinin induced an antibody response biased towards IgG2a, indicating a Th1 type of response. In most instances the mixing of plasmids had only slight effects on the magnitude or bias of the responses to the individual components. However, under certain conditions we found that addition of a second plasmid converted an IgG2a biased response to a response with primarily IgG1 antibody. The reverse situation (i.e. an IgG1 conversion to IgG2a), however, was not found. These findings have important implications for the development of multivalent vaccines.

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1998-12-01
2024-04-19
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References

  1. Babiuk L. A., Lewis P. J., van Drunen Littel-van den Hurk S., Tikoo S., Liang X. 1998; Nucleic acid vaccines: veterinary applications. Current Topics in Microbiology and Immunology 226:90–106
    [Google Scholar]
  2. Braun R., Babiuk L. A., van Drunen Littel-van den Hurk S. 1997; Enhanced immune response to an intradermally delivered DNA vaccine expressing a secreted form of BHV-1 gD. Vaccine Research 6:151–164
    [Google Scholar]
  3. Breker-Klassen M. M., Yoo D., Mittal S. K., Sorden S. D., Haines D. M., Babiuk L. A. 1995; Recombinant type 5 adenovirus expressing bovine parainfluenza virus type 3 glycoproteins protect Sigmodon hispidus cotton rats from bovine parainfluenza virus type 3 infection. Journal of Virology 69:4308–4315
    [Google Scholar]
  4. Cardoso A. I., Sixt N., Vallier A., Fayolle J., Buckland R., Wild T. F. 1998; Measles virus DNA vaccination: antibody isotype is determined by the method of immunization and by the nature of both the antigen and the coimmunized antigen. Journal of Virology 72:2516–2518
    [Google Scholar]
  5. Doe B., Selby M., Barnett S., Baenziger J., Walker C. M. 1996; Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells. Proceedings of the National Academy of Sciences, USA 93:8578–8583
    [Google Scholar]
  6. Feltquate D. M., Heaney S., Webster R. G., Robinson H. L. 1997; Different T helper cell types and antibody isotypes generated by saline and gene gun DNA immunization. Journal of Immunology 158:2278–2284
    [Google Scholar]
  7. Gerdts V., Jons A., Makoschey B., Visser N., Mettenleiter T. C. 1997; Protection of pigs against Aujeszky’s disease by DNA vaccination. Journal of General Virology 78:2139–2146
    [Google Scholar]
  8. Hassett D. E., Whitton J. L. 1996; DNA immunization. Trends in Microbiology 4:307–312
    [Google Scholar]
  9. Hoskins J. M. 1967; Embryonated egg techniques. In Virological Procedures pp. 93–104 Hoskins J. M. Edited by London: Butterworth;
    [Google Scholar]
  10. Kowalski J., Gilbert S. A., van Drunen Littel-van den Hurk S., Babiuk L. A., Zamb T. J. 1993; Heat-shock promoter-driven synthesis of secreted bovine herpesvirus glycoproteins in transfected cells. Vaccine 11:1100–1107
    [Google Scholar]
  11. Li Y., van Drunen Littel-van den Hurk S., Liang X., Babiuk L. A. 1996; Production and characterization of bovine herpesvirus 1 glycoprotein B ectodomain derivatives in an HSP70A gene promoter- based expression system. Archives of Virology 141:2019–2029
    [Google Scholar]
  12. McClements W. L., Armstrong M. E., Keys R. D., Liu M. A. 1996; Immunization with DNA vaccines encoding glycoprotein D or glycoprotein B, alone or in combination, induces protective immunity in animal models of herpes simplex virus-2 disease. Proceedings of the National Academy of Sciences, USA 93:11414–11420
    [Google Scholar]
  13. Manickan E., Karem K. L., Rouse B. T. 1997; DNA vaccines - a modern gimmick or a boon to vaccinology?. Critical Reviews in Immunology 17:139–154
    [Google Scholar]
  14. Montgomery D. L., Shiver J. W., Leander K. R., Parry H. C., Friedman A., Martinez D., Ulmer J. B., Donnelly J. J., Liu M. A. 1993; Heterolog ous and homologous protection against influenza A by DNA vaccination: optimization of DNA vectors. DNA and Cell Biology 12:777–783
    [Google Scholar]
  15. Mosmann T. R., Sad S. 1996; The expanding universe of T-cell subsets: Th1, Th2 and more. Immunology Today 17:138–146
    [Google Scholar]
  16. Paul W. E., Seder R. A. 1994; Lymphocyte responses and cytokines. Cell 78:241–251
    [Google Scholar]
  17. Raz E., Tighe H., Sato Y., Corr M., Dudler J. A., Roman M., Swain S. L., Spiegelberg H. L., Carson D. A. 1996; Preferential induction of a Th1 immune response and inhibition of specific IgE antibody formation by plasmid DNA immunization. Proceedings of the National Academy of Sciences, USA 93:5141–5145
    [Google Scholar]
  18. Robinson H. L., Torres C. A. T. 1997; DNA vaccines. Seminars in Immunology 9:271–283
    [Google Scholar]
  19. Roman M., Martin-Orozco E., Goodman J. S., Nguyen M., Sato Y., Ronaghy A., Kornbluth R. S., Richman D. D., Carson D. A., Raz E. 1997; Immunostimulatory DNA sequences function as T helper-1-promoting adjuvants. Nature Medicine 3:849–854
    [Google Scholar]
  20. Sato Y., Roman M., Tighe H., Lee D., Corr M., Nguyen M., Silverman G. J., Lotz M., Carson D. A., Raz E. 1996; Immuno-stimulatory DNA sequences necessary for effective intradermal gene immunization. Science 273:352–354
    [Google Scholar]
  21. Suzu S., Sakai Y., Shioda T., Shibuta H. 1987; Nucleotide sequence of the bovine parainfluenza 3 genome : the genes of the F and HN glycoproteins. Nucleic Acids Research 15:2945–2958
    [Google Scholar]
  22. Tikoo S. K., Fitzpatrick D., Babiuk L. A., Zamb T. J. 1990; Molecular cloning, sequencing and expression of functional bovine herpesvirus 1 glycoprotein gIV in transfected bovine cells. Journal of Virology 64:5132–5142
    [Google Scholar]
  23. Whalen R. G., Davis H. L. 1995; DNA-mediated immunization and the energetic immune response to hepatitis B surface antigen. Clinical Immunology and Immunopathology 75:1–12
    [Google Scholar]
  24. Whitbeck J. C., Bello L. J., Lawrence W. C. 1988; Comparison of the bovine herpesvirus 1 gI gene and the herpes simplex virus type 1 gB gene. Journal of Virology 62:3319–3327
    [Google Scholar]
  25. Young J. F., Desselberger U., Graves P., Palese P., Shatzman A., Rosenberger M. 1983; Cloning and expression of influenza virus genes. In The Origin of Pandemic Influenza Viruses pp. 129–138 Laver W. G. Edited by Amsterdam: Elsevier Science Publishing;
    [Google Scholar]
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