1887

Abstract

In this report the influence of the cellular stress response in mediating changes in human T-lympho-tropic virus type I (HTLV-I) viral envelope (Env) protein metabolism is determined. Previously, we reported that induction of the cellular stress response enhanced HTLV-I-mediated syncytia formation following induction of the cellular stress response in persistently infected lymphocytes. In this study, we show that the increase in HTLV-I-mediated syncytia formation following stress response induction is a result of increased cell surface expression of viral Env protein (gp46). Cellular stress in MT 2.6 cells did not alter the turnover of intracellular Env protein (gp68) as no changes in viral protein half-life were demonstrated as compared to non-stressed cells. However, Env expression in stressed cells treated with a protein synthesis inhibitor (cycloheximide) indicates the effect is mediated through increased translation of viral Env protein.

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/content/journal/jgv/10.1099/0022-1317-79-12-2905
1998-12-01
2022-01-20
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References

  1. Andrews J. M., Newbound G. C., Oglesbee M., Brady J. N., Lairmore M. D. 1997; The cellular stress response enhances human T-cell lymphotropic virus type 1 basal gene expression through the core promoter region of the long terminal repeat. Journal of Virology 71:741–745
    [Google Scholar]
  2. Andrews J. M., Oglesbee M. J., Trevino A. V., Guyot D. J., Newbound G. C., Lairmore M. D. 1995; Enhanced human T-cell lymphotropic virus type 1 expression following induction of the cellular stress response. Virology 208:816–820
    [Google Scholar]
  3. Ballinger D. G., Pardue M. L. 1983; The control of protein synthesis during heat shock in Drosophila cells involves altered polypeptide elongation rates. Cell 33:103–113
    [Google Scholar]
  4. Gadzar A. F., Carney D. N., Bunn P. A., Russell E. K., Jaffe E. S., Schechter G. P., Guccion J. G. 1980; Mitogen requirements for in vitro propagation of cutaneous T-cell lymphomas. Blood 55:409–417
    [Google Scholar]
  5. Hartley T. M., Khabbaz R. F., Cannon R. O., Kaplan J. E., Lairmore M. D. 1990; Characterization of antibody reactivity to human T-cell lymphotropic virus types I and II using immunoblot and radioimmuno- precipitation assays. Journal of Clinical Microbiology 28:646–650
    [Google Scholar]
  6. Hattori S., Kiyoka T., Imagawa K., Shimizu F., Hashimura E., Seiki M., Yoshida M. 1984; Identification of gag and env gene products of human T-cell leukemia virus (HTLV). Virology 136:338–347
    [Google Scholar]
  7. Hickey E., Weber L. A. 1982; Preferential translation of heat shock mRNAs in HeLa cells. In Heat Shock from Bacteria to Man p. 440 Schlesinger M. J., Ashburner M., Tissieres A. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press;
    [Google Scholar]
  8. Lindquist S. 1986; The heat-shock response. Annual Review of Biochemistry 55:1151–1191
    [Google Scholar]
  9. Luscher B., Eisenman R. 1988; c-myc and c-myb protein degradation: effect of metabolic inhibitors and heat shock. Molecular and Cellular Biology 8:2504–2512
    [Google Scholar]
  10. McKenzie S. L., Henikoff K., Meselson M. 1975; Localization of RNA from heat-shocked induced polysomes at puff sites in Drosophila melanogaster. Proceedings of the National Academy of Sciences, USA 72:1117–1121
    [Google Scholar]
  11. Miyoshi I., Kubonishi I., Yoshimoto S., Akagi T., Ohtsuki Y., Shiraishi Y., Nagata K., Hinuma Y. 1981; Type C virus particles in cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukemia T cells. Nature 294:770–773
    [Google Scholar]
  12. Nagy K., Clapham P., Cheingsong-Popov R., Weiss R. A. 1983; Human T-cell leukemia virus type I : induction of syncytia and inhibition by patients’ sera. International Journal of Cancer 32:321–328
    [Google Scholar]
  13. Nover L. 1991; Inducers of hsp synthesis : heat shock and chemical stressors. In Heat Shock Response pp. 6–40 Nover L. Edited by Boca Raton, Florida: CRC Press;
    [Google Scholar]
  14. Paine E., Gu R., Ratner L. 1994; Structure and expression of the human T-cell leukemia virus type I envelope protein. Virology 199:331–338
    [Google Scholar]
  15. Palker T. J., Tanner M. E., Scearce R. M., Clark B. F. 1989; Mapping of immunogenic regions of human T cell leukemia virus type I. Journal of Immunology 142:971–978
    [Google Scholar]
  16. Panniers R. 1994; Translational control during heat shock. Biochimie 76:737–747
    [Google Scholar]
  17. Panniers R., Henshaw E. C. 1984; Mechanism of inhibition of polypeptide chain initiation in heat shocked Ehrlich ascites tumor cells. European Journal of Biochemistry 140:209–214
    [Google Scholar]
  18. Pique C., Pham D., Tursz T., Dokhelar M.-C. 1992; Human T-cell leukemia virus type I envelope protein maturation process : requirements for syncytia formation. Journal of Virology 66:906–913
    [Google Scholar]
  19. Schneider J., Yamamoto N., Hinuma Y., Hunsmann G. 1984; Sera from adult T-cell leukemia patients react with envelope and core polypeptides of adult T-cell leukemia virus. Virology 132:1–11
    [Google Scholar]
  20. Storti R. V., Scott M. P., Rich A., Pardue M. L. 1980; Translational control of protein synthesis in response to heat shock in D. melanogaster cells. Cell 22:825–834
    [Google Scholar]
  21. Theodorakis N. G., Banerji S. S., Morimoto R. I. 1988; Hsp70 mRNA translation in chicken reticulocytes is regulated at the level of elongation. Journal of Biological Chemistry 263:14579–14585
    [Google Scholar]
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