1887

Abstract

The genome organization of the single-nucleocapsid nucleopolyhedrovirus (BusuNPV) was largely elucidated and compared to those of other baculoviruses. A detailed physical map was constructed for the restriction enzymes HI, I, II, RI, dIII, I, I, I and I. The 120·9 kbp viral genome was cloned as restriction fragments into a plasmid library from which about 43·5 kbp of dispersed sequence information was generated. Fifty-two putative open reading frames homologous to those of other baculoviruses were identified and their location in the genome of BusuNPV was determined. Although the gene content of BusuNPV is similar to that of multiple-nucleocapsid nucleopolyhedrovirus, nucleopolyhedrovirus and multiple-nucleocapsid nucleopolyhedrovirus, the gene order is, however, significantly different from that observed in the other viruses, which have a high degree of collinearity. A new approach (GeneParity-Plot) was developed to represent the differences in gene order among baculoviruses when limited sequence information is available and to take advantage of the high degree of gene conservation. The data obtained show that BusuNPV is a distinct baculovirus species and the analyses suggest that gene distribution along baculovirus genomes may be used as a phylogenetic marker.

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/content/journal/jgv/10.1099/0022-1317-79-11-2841
1998-11-01
2022-05-23
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