1887

Abstract

Most animal rotaviruses bind to a cell surface molecule that contains sialic acid (SA). We have recently isolated variants from simian rotavirus RRV which show an SA-independent phenotype. The VP4 protein of these variants was shown to have three amino acid changes with respect to the wt protein, one of them being Tyr-267 → Cys. In this work, we have investigated whether the new cysteine could interfere with the disulfide bond (Cys-318/Cys-380) present in the VP5 * subunit of the wt protein. Cysteine residues 318 and 380 were mutagenized in gpr8 and RRV VP4 genes, and the wt and mutant genes were transcribed and translated The protein products were analysed by electrophoresis under reducing and non-reducing conditions. This approach showed that, in the VP4 protein synthesized , Cys- 267 is capable of forming an alternate disulfide bond with Cys-318. This alternate bond also seems to occur in the VP4 protein present in the variant gpr8 virus particles.

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1998-11-01
2024-04-24
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References

  1. Arias C. F., Romero P., Alvarez V., López S. 1996; Trypsin activation pathway of rotavirus infectivity. Journal of Virology 70:5832–5839
    [Google Scholar]
  2. Coulson B. S., Londrigan S. H., Lee D. J. 1997; Rotavirus contains integrin ligand sequences and a disintegrin-like domain implicated in virus entry into cells. Proceedings of the National Academy of Sciences, USA 94:5389–5394
    [Google Scholar]
  3. Dedera D., Ruili G., Ratner L. 1992; Conserved cysteine residues in the human immunodeficiency virus type 1 transmembrane envelope protein are essential for precursor envelope cleavage. Journal of Virology 66:1207–1209
    [Google Scholar]
  4. De Zoysa I., Feachem R. G. 1985; Interventions for the control of diarrhoeal diseases among young children: rotavirus and cholera immunization. Bulletin of the World Health Organization 63:569–583
    [Google Scholar]
  5. Espejo R., Martinez E., López S., Munoz O. 1980; Different polypeptide composition of two human rotavirus types. Infection and Immunity 28:230–237
    [Google Scholar]
  6. Estes M. K., Cohen J. 1989; Rotavirus gene structure and function. Microbiological Reviews 53:410–449
    [Google Scholar]
  7. Faaberg K. S., Even C., Palmer G. A., Plagemann P. G. 1995; Disulfide bonds between two enveloped proteins of lactate de-hydrogenase-elevating virus are essential for viral infectivity. Journal of Virology 69:613–617
    [Google Scholar]
  8. Fiore L., Greenberg H. B., Mackow E. R. 1991; The VP8 fragment of VP4 is the rhesus rotavirus hemagglutinin. Virology 181:553–563
    [Google Scholar]
  9. Fukudome K., Yoshie O., Konno T. 1989; Comparison of human, simian, and bovine rotaviruses for requirement of sialic acid in hemagglutination and cell absorption. Virology 172:196–205
    [Google Scholar]
  10. Isa P., López S., Segovia L., Arias C. F. 1997; Functional and structural analysis of the sialic acid-binding domain of rotaviruses. Journal of Virology 71:6749–6756
    [Google Scholar]
  11. Keljo D. J., Smith A. K. 1988; Characterization of binding of simian rotavirus SA-11 to cultured epithelial cells. Journal of Pediatric Gastroenterology and Nutrition 7:249–256
    [Google Scholar]
  12. López S., Arias C. F., Bell J. R., Strauss J. H., Espejo R. T. 1985; Primary structure of the cleavage site associated with trypsin enhancement of rotavirus SA11 infectivity. Virology 144:11–19
    [Google Scholar]
  13. López S., Espinosa R., Greenberg H. B., Arias C. F. 1994; Mapping the subgroup epitopes of rotavirus protein VP6. Virology 204:153–162
    [Google Scholar]
  14. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Dang M. N., Greenberg H. B. 1988; The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region. Proceedings of the National Academy of Sciences, USA 85:645–649
    [Google Scholar]
  15. Méndez E., Arias C. F., López S. 1993; Binding to sialic acids is not an essential step for the entry of animal rotaviruses to epithelial cells in culture. Journal of Virology 67:5253–5259
    [Google Scholar]
  16. Méndez E., Arias C. F., López S. 1996; Interactions between the two surface proteins of rotavirus may alter the receptor-binding specificity of the virus. Journal of Virology 70:1218–1222
    [Google Scholar]
  17. Padilla-Noriega L., Werner-Eckert R., Mackow E. R., Gorziglia M., Larralde G., Taniguchi K., Greenberg H. B. 1993; Serologic analysis of human rotavirus serotypes P1A and P2 by using monoclonal antibodies. Journal of Clinical Microbiology 31:622–628
    [Google Scholar]
  18. Patton J. T., Hua J., Mansell E. A. 1993; Location of intrachain disulfide bonds in the VP5* and VP8* trypsin cleavage fragments of the rhesus rotavirus spike protein VP4. Journal of Virology 67:4848–4855
    [Google Scholar]
  19. Ruggieri F. M., Greenberg H. B. 1991; Antibodies to the trypsin cleavage peptide VP8 neutralize rotavirus by inhibiting binding of virions to target cells in culture. Journal of Virology 65:2211–2219
    [Google Scholar]
  20. Thomas A., Roth M. J. 1995; Analysis of cysteine mutations on the transmembrane protein of Moloney murine leukemia virus. Virology 211:285–289
    [Google Scholar]
  21. Tschachler E., Buchow H., Gallo R. G., Reitz M. S. Jr 1990; Functional contribution of cysteine residues to the human immuno-deficiency virus type 1 envelope. Journal of Virology 64:2250–2259
    [Google Scholar]
  22. Yolken R. H., Willoughby R., Wee S. B., Miskuff R., Vonderfecht S. 1987; Sialic acid glycoproteins inhibit in vitro and in vivo replication of rotaviruses. Journal of Clinical Investigation 79:148–154
    [Google Scholar]
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