1887

Abstract

The pathogenesis of pneumonia virus of mice (PVM) and human respiratory syncytial virus (HRSV) in BALB/c mice were investigated by using hybridization to detect virus mRNA in fixed lung sections. Following intranasal inoculation with 120 p.f.u. PVM the pattern of hybridization showed that virus mRNA was initially detected within 2 days in alveolar cells. As the infection progressed the number of hybridizing alveolar cells increased and signal was also detected in cells lining the terminal bronchioles. By days 4 to 5 post-infection areas of morphological abnormality could be seen, particularly in the strongly hybridizing regions of the lung, and this correlated with the appearance of clinical signs of infection. In animals which survived the infection virus-specific mRNA could not be detected 10 days post-infection. Mice infected with 1500 p.f.u. HRSV showed significant differences in the distribution of virus-specific mRNA when compared to the pattern seen with PVM. HRSV mRNA was detected over large areas, but predominantly in peribronchiolar and perivascular regions of the lungs 5 days post-infection. The yield of PVM from infected mouse lungs was considerably higher than that of HRSV. The possible implications of these results for the use of the mouse model for pneumovirus infections are discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-10-2411
1998-10-01
2022-08-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/10/9780046.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-10-2411&mimeType=html&fmt=ahah

References

  1. Aherne W., Bird T., Court S. D. M., Gardiner P. S., McQuillin J. 1970; Pathological changes in virus infections of the lower respiratory tract in children. Journal of Clinical Pathology 23:7–18
    [Google Scholar]
  2. Anderson J. J., Norden J., Saunders D., Toms G. L., Scott R. 1990; Analysis of the local and systemic immune responses induced in BALB/c mice by experimental respiratory syncytial virus infection. Journal of General Virology 71:1561–1570
    [Google Scholar]
  3. Barr J., Chambers P., Pringle C. R., Easton A. J. 1991; Sequence of the major nucleocapsid protein gene of pneumonia virus of mice: sequence comparisons suggest structural homology between nucleo- capsid proteins of pneumoviruses, paramyxoviruses, rhabdoviruses and filoviruses. Journal of General Virology 72:677–685
    [Google Scholar]
  4. Cannon M. J., Openshaw P. J. M., Askonas B. A. 1988; Cytotoxic T-cells clear virus but augment lung pathology in mice infected with respiratory syncytial virus. Journal of Experimental Medicine 168:1163–1168
    [Google Scholar]
  5. Cannon M. J., Openshaw P. J. M., Askonas B. A. 1989; Lethal pulmonary disease in respiratory syncytial virus-infected mice following transfer of cytotoxic T-cells. Vaccine 89:497–498
    [Google Scholar]
  6. Carthew P., Sparrow S. 1980a; A comparison in germ-free mice of the pathogenesis of Sendai virus and mouse pneumonia virus infections. Journal of Pathology 130:153–58
    [Google Scholar]
  7. Carthew P., Sparrow S. 1980b; Persistence of pneumonia virus of mice and Sendai virus in germ-free (nu/nu) mice. British Journal of Experimental Pathology 61:172–175
    [Google Scholar]
  8. Chambers P., Pringle C. R., Easton A. J. 1990; Molecular cloning of pneumonia virus of mice. Journal of Virology 64:1869–1872
    [Google Scholar]
  9. Chambers P., Pringle C. R., Easton A. J. 1991; Genes 1 and 2 of pneumonia virus of mice encode proteins which have little homology with the 1C and 1B proteins of human respiratory syncytial virus. Journal of General Virology 72:2545–2549
    [Google Scholar]
  10. Easton A. J., Eglin R. P. 1991; In situ hybridization. In Methods in Gene Technology 1 pp 185–202 Dale J. W., Sanders P. G. Edited by London: JAI Press;
    [Google Scholar]
  11. Eaton M. D., van Herick W. 1944; Demonstration in cotton rats and rabbits of a latent virus related to pneumonia virus of mice. Proceedings of the Society for Experimental Biology and Medicine 57:89–92
    [Google Scholar]
  12. Gimenez H. B., Cash P., Melvin W. T. 1984; Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates. Journal of General Virology 65:963–971
    [Google Scholar]
  13. Homberger F. R., Thomann P. E. 1994; Transmission of murine viruses and mycoplasma in laboratory mouse colonies with respect to housing conditions. Laboratory Animals 28:113–120
    [Google Scholar]
  14. Horsfall F. L., Curnen E. C. 1946; Studies on pneumonia virus of mice (PVM). II. Immunological evidence of latent infection with virus in numerous mammalian species. Journal ofExperimental Medicine 83:43–64
    [Google Scholar]
  15. Horsfall F. L., Ginsberg H. S. 1951; The dependence of the pathological lesion upon the multiplication of pneumonia virus of mice. Journal of Experimental Medicine 93:139–150
    [Google Scholar]
  16. Kraft V., Meyer B. 1990; Seromonitoring in small laboratory animal colonies. A five year survey: 1984-1988. Zeitschrift für Versuchstierkunde 33:29–35
    [Google Scholar]
  17. Ling R., Pringle C. R. 1989; Polypeptides of pneumonia virus of mice. I. Immunological cross-reactions and post-translational modifi-cations. Journal of General Virology 70:1427–1440
    [Google Scholar]
  18. McIntosh K., Chanock R. M. 1990 Respiratory Syncytial Virus, 2nd edn.. pp 1045–1072 New York: Raven Press;
    [Google Scholar]
  19. Murphy B. R., Prince G. A., Lawrence L. A., Croen K. D., Collins P. L. 1990; Detection of respiratory syncytial virus infected cells by in situ hybridization in the lungs of cotton rats immunized with formalin- inactivated virus or purified RSV F and G glycoprotein subunit vaccine and challenged with RSV. Virus Research 16:153–162
    [Google Scholar]
  20. Pringle C. R. 1996; Virus taxonomy 1996 - a bulletin from the Xth International Congress ofVirology in Jerusalem. ArchivesofVirology 141:2251–2256
    [Google Scholar]
  21. Pringle C. R., Eglin R. P. 1986; Murine pneumonia virus : seroepidemiological evidence of widespread human infection. Journal of General Virology 67:975–982
    [Google Scholar]
  22. Richter C. B., Thigpen J. E., Richter C. S., Mackenzie J. M. 1988; Fatal pneumonia with terminal emaciation in nude mice caused by pneumonia virus of mice. Laboratory Animal Science 38:255–261
    [Google Scholar]
  23. Roberts S. R., Compans R. W., Wertz G. W. 1995; Respiratory syncytial virus matures at the apical surfaces of polarized epithelial-cells. Journal of Virology 69:2667–2673
    [Google Scholar]
  24. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn.. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Smith A. L., Carrano V. A., Brownstein D. G. 1984; Response of weanling random-bred mice to infection with pneumonia virus of mice. Laboratory Animal Science 38:133–137
    [Google Scholar]
  26. Taylor G., Stott E. J., Hughes M., Collins A. P. 1984; Respiratory syncytial virus infection in mice. Infection and Immunity 43:649–655
    [Google Scholar]
  27. Weir E. C., Brownstein D. G., Smith A. L., Johnson E. A. 1988; Respiratory disease and wasting in athymic mice infected with pneumonia virus of mice. Laboratory Animal Science 34:35–37
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-10-2411
Loading
/content/journal/jgv/10.1099/0022-1317-79-10-2411
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error