RT Journal Article SR Electronic(1) A1 Reavy, B. A1 Arif, M. A1 Cowan, G. H. A1 Torrance, L.YR 1998 T1 Association of sequences in the coat protein/readthrough domain of potato mop-top virus with transmission by Spongospora subterranea JF Journal of General Virology, VO 79 IS 10 SP 2343 OP 2347 DO https://doi.org/10.1099/0022-1317-79-10-2343 PB Microbiology Society, SN 1465-2099, AB A monofungal culture of Spongospora subterranea was unable to acquire and transmit the T isolate of potato mop-top pomovirus (PMTV-T), which has been maintained by manual transmission in the laboratory for 30 years. A recently obtained field isolate (PMTV-S) was efficiently acquired and transmitted by the same fungus culture. Sequence analysis of the readthrough (RT) protein-coding region of PMTV-S showed the presence of an additional 543 nt in the 3′ half of the coding region relative to that of PMTV-T. These additional nucleotides preserved the reading frame of the RT protein and inserted 181 amino acids into the RT protein. This was confirmed by a comparison by immuno- blotting of the sizes of the RT protein of PMTV-T and other recent isolates of PMTV., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-10-2343