1887

Abstract

A monofungal culture of was unable to acquire and transmit the T isolate of potato mop-top pomovirus (PMTV-T), which has been maintained by manual transmission in the laboratory for 30 years. A recently obtained field isolate (PMTV-S) was efficiently acquired and transmitted by the same fungus culture. Sequence analysis of the readthrough (RT) protein-coding region of PMTV-S showed the presence of an additional 543 nt in the 3′ half of the coding region relative to that of PMTV-T. These additional nucleotides preserved the reading frame of the RT protein and inserted 181 amino acids into the RT protein. This was confirmed by a comparison by immuno- blotting of the sizes of the RT protein of PMTV-T and other recent isolates of PMTV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-10-2343
1998-10-01
2022-08-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/10/9780038.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-10-2343&mimeType=html&fmt=ahah

References

  1. Arif M., Torrance L., Reavy B. 1994; Improved efficiency of detection of potato mop-top furovirus in potato tubers and in the roots and leaves of soil-bait plants. Potato Research 37:373–381
    [Google Scholar]
  2. Arif M., Torrance L., Reavy B. 1995; Acquisition and transmission of potato mop-top furovirus by a culture of Spongospora subterranea f. sp. subterranea derived from single cystosorus. Annals of Applied Biology 126:493–503
    [Google Scholar]
  3. Bouzoubaa S., Ziegler V., Beck D., Guilley H., Richards K., Jonard G. 1986; Nucleotide sequence of beet necrotic yellow vein virus RNA- 2. Journal of General Virology 67:1689–1700
    [Google Scholar]
  4. Chen J., MacFarlane S. A., Wilson T. M. A. 1994; Detection and sequence analysis of a spontaneous deletion mutant of soil-borne wheat mosaic virus RNA 2 associated with increased symptom severity. Virology 202:921–929
    [Google Scholar]
  5. Cowan G. H., Torrance L., Reavy B. 1997; Detection of potato mop-top virus capsid readthrough protein in virus particles. Journal of General Virology 78:1779–1783
    [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  7. Haeberle A. M., Stussi-Garaud C., Schmitt C., Garaud J. C., Richards K. E., Guilley H., Jonard G. 1994; Detection by immunogold labelling of P75 readthrough protein near an extremity of beet necrotic yellow vein virus particles. Archives of Virology 134:195–203
    [Google Scholar]
  8. Harrison B. D., Jones R. A. C. 1970; Host range and properties of potato mop-top virus. Annals of Applied Biology 65:393–402
    [Google Scholar]
  9. Jones R. A. C. 1988; Epidemiology and control of potato mop-top virus. In Developments inApplied Biology II Viruses with Fungal Vectors pp 255–270 Cooper J. I., Asher M. J. C. Edited by Wellesbourne: Association of Applied Biologists;
    [Google Scholar]
  10. Jones R. A. C., Harrison B. D. 1969; The behaviour of potato moptop virus in soil, and evidence for its transmission by Spongospora subterranea (Wallr.) Lagerh. Annals of Applied Biology 63:1–17
    [Google Scholar]
  11. Kashiwazaki S., Scott K. P., Reavy B., Harrison B. D. 1995; Sequence analysis and gene organization of potato mop-top virus RNA- 3. Virology 206:701–706
    [Google Scholar]
  12. Koenig R., Commandeur S., Loss S., Beier C., Kaufmann A., Lesemann D.-E. 1997; Beet soil-borne virus RNA 2: similarities and dissimilarities to the coat protein gene-carrying RNAs of other furoviruses. Journal of General Virology 78:469–477
    [Google Scholar]
  13. Mayo M. A., Torrance L., Cowan G., Jolly C. A., Macintosh S. M., Orrega R., Barrera C., Salazar L. F. 1996; Conservation of coat protein sequence among isolates of potato mop-top virus from Scotland and Peru. Archives of Virology 141:1115–1121
    [Google Scholar]
  14. Merz U. 1989; Infectivity, inoculum density and germination of Spongospora subterranea resting spores : a solution-culture test system. EPPO Bulletin 19:585–592
    [Google Scholar]
  15. Schmitt C., Balmori E., Jonard G., Richards K. E., Guilley H. 1992; In vitro mutagenesis of biologically active transcripts of beet necrotic yellow vein virus RNA 2: evidence that a domain of the 75-kDa readthrough protein is important for efficient virus assembly. Proceedings of the National Academy of Sciences, USA 89:5715–5719
    [Google Scholar]
  16. Scott K. P., Kashiwazaki S., Reavy B., Harrison B. D. 1994; The nucleotide sequence of potato mop-top virus RNA 2 : a novel type of genome organization for a furovirus. Journal of General Virology 75:3561–3568
    [Google Scholar]
  17. Shirako Y., Brakke M. K. 1984; Spontaneous deletion mutation of soil-borne wheat mosaic virus RNA II. Journal of General Virology 65:855–858
    [Google Scholar]
  18. Shirako Y., Wilson T. M. A. 1993; Complete nucleotide sequence and organisation of the bipartite RNA genome of soil-borne wheat mosaic virus. Virology 195:16–32
    [Google Scholar]
  19. Tamada T., Kusume T. 1991; Evidence that the 75K readthrough protein of beet necrotic yellow vein virus RNA-2 is essential for transmission by the fungus Polymyxa betae. Journal of General Virology 72:1497–1504
    [Google Scholar]
  20. Tamada T., Schmitt C., Saito M., Guilley H., Richards K., Jonard G. 1996; High resolution analysis of the readthrough domain of beet necrotic yellow vein virus readthrough protein: a KTER motif is important for efficient transmission of the virus by Polymyxa betae. Journal of General Virology 77:1359–1367
    [Google Scholar]
  21. Torrance L. 1992; Serological methods to detect plant viruses: production and use of monoclonal antibodies. In Techniques for the Rapid Detection of Plant Pathogens pp 7–33 Duncan J. M., Torrance L. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  22. Torrance L., Mayo M. A. 1997; Proposed re-classification of furoviruses. Archives of Virology 142:435–439
    [Google Scholar]
  23. Torrance L., Cowan G. H., Pereira L. G. 1993; Monoclonal antibodies specific for potato mop-top virus and some properties of the coat protein. Annals of Applied Biology 122:311–322
    [Google Scholar]
  24. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets : procedure and some applications. Proceedings of the National Academy of Sciences, USA 76:4350–4354
    [Google Scholar]
  25. Verwoerd T. C., Dekker B. M. M., Hoekema A. 1989; A small scale procedure for the rapid isolation of plant RNAs. NucleicAcids Research 17:2362
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-10-2343
Loading
/content/journal/jgv/10.1099/0022-1317-79-10-2343
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error