RT Journal Article SR Electronic(1) A1 Arbatova, Jelena A1 Lehto, Krishi A1 Pehu, Ejja A1 Pehu, TuulaYR 1998 T1 Localization of the P1 protein of potato Y potyvirus in association with cytoplasmic inclusion bodies and in the cytoplasm of infected cells JF Journal of General Virology, VO 79 IS 10 SP 2319 OP 2323 DO https://doi.org/10.1099/0022-1317-79-10-2319 PB Microbiology Society, SN 1465-2099, AB The N-terminal P1 proteinase of potato virus Y (ordinary strain group isolate PVY-O) was expressed in E. coli. Antiserum was raised against the expressed protein and used to detect the viral proteins in infected tobacco leaf tissue by Western blotting and by electron microscopy with immuno- gold labelling. In the immunogold localization studies P1 protein was detected in association with the cytoplasmic inclusion bodies characteristic of PVY infections and in the cytoplasm of the infected plant cells. No significant P1 antibody binding with other plant cell organelles, or with the cell wall and plasmodesmata, was detected by immunogold labelling., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-10-2319