Molecular characterization of a new whitefly-transmissible bipartite geminivirus infecting tomato in Panama Free

Abstract

The nucleotide sequence of infectious clones of a tomato-infecting geminivirus from Panama [named tomato leaf curl virus (ToLCV-Pan) because of symptoms produced in infected tomato (plant stunting and mild leaf curling)] was determined. ToLCV-Pan has a bipartite genome (DNAs A and B) and computer analysis showed that the genome resembles that of other bipartite, whitefly-trans- mitted geminiviruses. DNA A (2584 nt) and B (2542 nt) have little sequence homology other than within the common region. ToLCV-Pan clones were introduced into and infected plants developed the same symptoms as naturally infected tomatoes. Homology analysis of DNA A and B showed that ToLCV-Pan is most closely related to potato yellow mosaic virus (PYMV) from Venezuela. Pseudorecombination between ToLCV-Pan and PYMV did not give viable pseudorecombinant viruses. However, in some plants infected with the pseudorecombinant virus produced by ToLCV-Pan DNA A and PYMV DNA B, systemic movement of ToLCV-Pan DNA A was observed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-10-2313
1998-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/10/9780034.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-10-2313&mimeType=html&fmt=ahah

References

  1. Abouzid A. M., Polston J. E., Hiebert E. 1992; The nucleotide sequence of tomato mottle virus, a new geminivirus isolated from tomatoes in Florida. Journal of General Virology 73:3225–3229
    [Google Scholar]
  2. Brown J. K., Bird J., Banks G., Sosa M., Kiesler K., Cobrera I., Fornaris G. 1995; First report of an epidemic in tomato caused by two whitefly-transmitted geminiviruses in Puerto Rico. Plant Disease 79:1250
    [Google Scholar]
  3. Buragohain A. K., Sung Y. K., Coffin R. S., Coutts R. H. A. 1994; The infectivity of dimeric potato yellow mosaic geminivirus clones in different hosts. Journal of General Virology 75:2857–2861
    [Google Scholar]
  4. Coutts R. H. A., Coffin R. S., Roberts E. J. F., Hamilton W. D. O. 1991; The nucleotide sequence of the infectious cloned DNA components of potato yellow mosaic virus. Journal of General Virology 72:1515–1520
    [Google Scholar]
  5. Dry I. B., Rigden J. E., Krake L. R., Mullineaux P. M., Rezaian M. A. 1993; Nucleotide sequence and genome organization of tomato leaf curl geminivirus. Journal of General Virology 74:147–151
    [Google Scholar]
  6. Fontes E. P. B., Eagle P., Sipe P., Luckow V. A., Hanley-Bowdoin L. 1994a; Interaction between a geminivirus replication protein and origin DNA is essential for viral replication. Journal of Biological Chemistry 269:8459–8465
    [Google Scholar]
  7. Fontes E. P. B., Gladfelter H. J., Schaffer R. L., Petty I. T. D., Hanley-Bowdoin L. 1994b; Geminivirus replication origins have a modular organization. Plant Cell 6:405–416
    [Google Scholar]
  8. Frischmuth T., Stanley J. 1991; African cassava mosaic virus DI DNA interferes with the replication of both genomic components. Virology 183:539–544
    [Google Scholar]
  9. Frischmuth T., Zimmat G., Jeske H. 1990; The nucleotide sequence of Abutilon mosaic virus reveals prokaryotic as well as eukaryotic features. Virology 178:461–468
    [Google Scholar]
  10. Frischmuth T., Engel M., Lauster S., Jeske H. 1997; Nucleotide sequence evidence for the occurrence of three distinct whitefly- transmitted, Sida-infecting bipartite geminiviruses in Central America. Journal of General Virology 78:2675–2682
    [Google Scholar]
  11. Gilbertson R. L., Hidayat S. H., Paplomatas E. J., Rojas M. R., Hou Y.-M., Maxwell D. P. 1993; Pseudorecombination between infectious cloned DNA components of tomato mottle and bean dwarf mosaic geminiviruses. Journal of General Virology 74:23–31
    [Google Scholar]
  12. Hamilton W. D. O., Stein V. E., Coutts R. H. A., Buck K. W. 1984; Complete nucleotide sequence of the infectious cloned DNA components of tomato golden mosaic virus: potential coding regions and regulatory sequences. EMBO Journal 3:2197–2205
    [Google Scholar]
  13. Harrison B. D. 1985; Advances in geminivirus research. Annual Review of Phytopathology 23:5–82
    [Google Scholar]
  14. Höfer P., Engel M., Jeske H., Frischmuth T. 1997a; Nucleotide sequence of a new bipartite geminivirus isolated from the common weed Sida rhombifolia in Costa Rica. Journal of General Virology 78:1785–1790
    [Google Scholar]
  15. Höfer P., Engel M., Jeske H., Frischmuth T. 1997b; Host range limitation of a pseudorecombinant virus produced by two distinct bipartite geminiviruses. Molecular Plant-Microbe Interactions 10:1019–1022
    [Google Scholar]
  16. Howarth A. J., Caton J., Bossert M., Goodman R. M. 1985; Nucleotide sequence of bean golden mosaic virus and a model for gene regulation in geminiviruses. Proceedings ofthe NationalAcademy ofSciences, USA 82:3572–3576
    [Google Scholar]
  17. Lazarowitz S. G. 1992; Geminiviruses: genome structure and gene function. Critical Reviews in Plant Science 11:327–349
    [Google Scholar]
  18. Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martelli G. P., Mayo M. A., Summers M. D.editors 1995 Virus Taxonomy. Sixth Report ofthe International Committee on Taxonomy of Viruses pp 158–165 Vienna & New York: Springer-Verlag;
    [Google Scholar]
  19. Navot N., Pichersky E., Zeidan M., Zamir D., Czosnek H. 1991; Tomato yellow leaf curl virus : a whitefly-transmitted geminivirus with a single genomic component. Virology 185:151–161
    [Google Scholar]
  20. Padidam M., Beachy R. N., Fauquet C. M. 1995; Tomato leaf curl geminivirus from India has a bipartite genome and coat protein is not essential for infectivity. Journal of General Virology 76:25–35
    [Google Scholar]
  21. Paplomatas E. J., Patel V. P., Hou Y. M., Noueiry A. O., Gilbertson R. L. 1994; Molecular characterization of a new sap-transmissible bipartite genome geminivirus infecting tomatoes in Mexico. Phytopathology 84:1215–1224
    [Google Scholar]
  22. Polston J. E., Anderson P. K. 1997; The emergence of whitefly- transmitted geminiviruses in tomato in the western hemisphere. Plant Disease 81:1358–1369
    [Google Scholar]
  23. Simone G. W., Brown J. K., Hiebert E., Cullen R. E. 1990; New geminivirus epidemic in Florida tomatoes and peppers. Phytopathology 80:1063
    [Google Scholar]
  24. Stanley J., Frischmuth T., Ellwood S. 1990; Defective viral DNA ameliorates symptoms of geminiviruses infection in transgenic plants. Proceedings of the National Academy of Sciences, USA 87:6291–6295
    [Google Scholar]
  25. von Arnim A., Stanley J. 1992; Determinants of tomato golden mosaic virus symptom development located on DNA B. Virology 186:286–293
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-10-2313
Loading
/content/journal/jgv/10.1099/0022-1317-79-10-2313
Loading

Data & Media loading...

Most cited Most Cited RSS feed