1887

Abstract

Highly purified (> 98%) CD34 cells directly after isolation (D) or 2 weeks in culture (D) were CD4 and contained mRNA for the T-tropic HIV coreceptor, CXCR-4, and minor co-receptor, CCR-2B. D but not D cells were RT-PCR positive for mRNA for the major M-tropic human immunodeficiency virus (HIV) co-receptor, CCR-5, and potential coreceptor, CCR-1. D and D cells were susceptible to T- (HXB2) and M-tropic HIV (Bal), showing greater virus production with Bal than HXB2, and with higher virus production levels in D compared to D cells. Seven days post-infection of D cells Bal DNAwas present in CD14and CD14 fractions, suggesting D infection of diverse progenitor types. HXB2 DNA was detected in CD14 cells alone indicating D infection of monocyte progenitors only. It is concluded that CD34 cells and cultured derivatives are susceptible to M- and T-tropic HIV and this correlates in part with co-receptor expression at the mRNA level.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-1-71
1998-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/1/9460925.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-1-71&mimeType=html&fmt=ahah

References

  1. Aiuti A., Webb I. J., Bleul C., Springer T., Gutierrez-Ramos J. C. 1997; The chemokine SDF-1 is a chemoattractant for human CD34+ hematopoietic progenitor cells and provides a new mechanism to explain the mobilization of CD34+ progenitors to peripheral blood. Journal of Experimental Medicine 185:111–120
    [Google Scholar]
  2. Bleul C. C., Farzan M., Choe H., Parolin C., Clark-Lewis I., Sodroski J., Springer T. A. 1996; The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature 382:829–833
    [Google Scholar]
  3. Chelucci C., Hassan H. J., Locardi C., Bulgarini D., Pelosi E., Mariani G., Testa U., Federico M., Valtieri M., Peschle C. 1995; In vitro human immunodeficiency virus-1 infection of purified hematopoietic progenitors in single-cell culture. Blood 85:1181–1187
    [Google Scholar]
  4. Choe H., Farzan M., Sun Y., Sullivan N., Rollins B., Ponath P. D., Wu L., Mackay C. R., LaRosa G., Newman W., Gerard N., Gerard C., Sodroski J. 1996; The β-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates. Cell 85:1135–1148
    [Google Scholar]
  5. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidiniumthiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  6. Davis B. R., Schwartz D. H., Marx J. C., Johnson C. E., Berry J. M., Lyding J., Merigan T. C., Zander A. 1991; Absent or rare human immunodeficiency virus infection of bone marrow stem/progenitor cells in vivo . Journal of Virology 65:1985–1990
    [Google Scholar]
  7. Deichmann M., Kronenwett R., Haas R. 1997; Expression of the human immunodeficiency virus type-1 coreceptors CXCR-4 (fusin, LESTR) and CKR-5 in CD34+ hematopoietic progenitor cells. Blood 89:3522–3528
    [Google Scholar]
  8. De Luca A., Teofili L., Antinori A., Iovino M. S., Mencarini P., Visconti E., Tamburrini E., Leone G., Ortona L. 1993; Haemopoietic CD34+ progenitor cells are not infected by HIV-1 in vivo but show impaired clonogenesis. British Journal of Haematology 85:20–24
    [Google Scholar]
  9. Deng H.-K., Liu R., Ellmeier W., Choe S., Unutmaz D., Burkhart M., DiMarzio P., Marmon S., Sutton R. E., Hill C. M., Davis C. B., Peiper S. C., Schall T. J., Littman D. R., Landau N. R. 1996; Identification of a major co-receptor for primary isolates of HIV-1. Nature 381:661–666
    [Google Scholar]
  10. Doranz B. J., Rucker J., Yi Y., Smyth R. J., Samson M., Peiper S. C., Parmentier M., Collman R. G., Doms R. W. 1996; A dual-tropic primary HIV-1 isolate that used fusin and the β-chemokine receptors CKR-5, CKR-3 and CKR-2b as fusion cofactors. Cell 85:1149–1158
    [Google Scholar]
  11. Dragic T., Litwin V., Allaway G. P., Martin S. R., Huang Y., Nagashima K. A., Cayanan C., Maddon P. J., Koup R. A., Moore J. P., Paxton W. A. 1996; HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature 381:667–673
    [Google Scholar]
  12. Feng Y., Broder C. C., Kennedy P. E., Berger E. A. 1996; HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 272:872–877
    [Google Scholar]
  13. Folks T. M., Kessler S. W., Orenstein J. M., Justement J. S., Jaffe E. S., Fauci A. S. 1988; Infection and replication of HIV-1 in purified progenitor cells of normal human bone marrow. Science 242:919–922
    [Google Scholar]
  14. Haylock D. N., To L. B., Dowse T. L., Juttner C. A., Simmons P. J. 1992; Ex vivo expansion and maturation of peripheral blood CD34+ cells into the myeloid lineage. Blood 80:1405–1412
    [Google Scholar]
  15. Kaushal S., La Russa V. F., Gartner S., Kessler S., Perfetto S., Yu S., Ritchey D. W., Xu J., Perera P., Kim J., Reid T., Mayers D. L., St Louis D., Mosca J. D. 1996; Exposure of human CD34+ cells to human immunodeficiency virus type 1 does not influence their expansion and proliferation of hematopoietic progenitors in vitro . Blood 88:130–137
    [Google Scholar]
  16. Kitano K., Abboud C. N., Ryan D. H., Quan S. G., Baldwin G. C., Golde D. W. 1991; Macrophage-active colony stimulating factors enhance human immunodeficiency virus type 1 infection in bone marrow stem cells. Blood 77:1699–1705
    [Google Scholar]
  17. Loetscher M., Geiser T., O’Reilly T., Zwahlen R., Baggiolini M., Moser B. 1994; Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. Journal of Biological Chemistry 269:232–237
    [Google Scholar]
  18. Louache F., Debili N., Marandin A., Coulombel L., Vainchenker W. 1994; Expression of CD4 by human hematopoietic progenitors. Blood 84:3344–3355
    [Google Scholar]
  19. Molina J.-M., Scadden D. T., Sakaguchi M., Fuller B., Woon A., Groopman J. E. 1990; Lack of evidence for infection of or effect on growth of hematopoietic progenitor cells after in vivo or in vitro exposure to human immunodeficiency virus. Blood 76:2476–2482
    [Google Scholar]
  20. Oberlin E., Amara A., Bachelerie F., Bessia C., Virelizier J.-L., Arenzana-Seisdedos F., Schwartz O., Heard J.-M., Clark-Lewis I., Legler D. F., Loetscher M., Baggiolini M., Moser B. 1996; The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line adapted HIV-1. Nature 382:833–835
    [Google Scholar]
  21. Pietroboni G. R., Harnett G. B., Bucens M. R. 1989; Centrifugal enhancement of human immunodeficiency virus (HIV) and human herpes virus type 6 (HHV-6) infection in vitro . Journal of Virological Methods 24:85–90
    [Google Scholar]
  22. Stanley S. K., Kessler S. W., Justement J. S., Schnittman S. M., Greenhouse J. J., Brown C. C., Musongela L., Musey K., Kapita B., Fauci A. S. 1992; CD34+ bone marrow cells are infected with HIV in a subset of seropositive individuals. Journal of Immunology 149:689–697
    [Google Scholar]
  23. Steinberg H. N., Crumpacker C. S., Chatis P. A. 1991; In vitro suppression of normal human bone marrow progenitor cells by human immunodeficiency virus. Journal of Virology 65:1765–1769
    [Google Scholar]
  24. Tjonnfjord G. E., Steen R., Veiby O. P., Egeland T. 1996; Lineage commitment of CD34+ human hematopoietic progenitor cells. Experimental Haematology 24:875–882
    [Google Scholar]
  25. von Laer D., Hufert F. T., Fenner T. E., Schwander S., Dietrich M., Schmitz H., Kern P. 1990; CD34+ hematopoietic progenitor cells are not a major reservoir of the human immunodeficiency virus. Blood 76:1281–1286
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-1-71
Loading
/content/journal/jgv/10.1099/0022-1317-79-1-71
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error