1887

Abstract

Highly purified (> 98%) CD34 cells directly after isolation (D) or 2 weeks in culture (D) were CD4 and contained mRNA for the T-tropic HIV coreceptor, CXCR-4, and minor co-receptor, CCR-2B. D but not D cells were RT-PCR positive for mRNA for the major M-tropic human immunodeficiency virus (HIV) co-receptor, CCR-5, and potential coreceptor, CCR-1. D and D cells were susceptible to T- (HXB2) and M-tropic HIV (Bal), showing greater virus production with Bal than HXB2, and with higher virus production levels in D compared to D cells. Seven days post-infection of D cells Bal DNAwas present in CD14and CD14 fractions, suggesting D infection of diverse progenitor types. HXB2 DNA was detected in CD14 cells alone indicating D infection of monocyte progenitors only. It is concluded that CD34 cells and cultured derivatives are susceptible to M- and T-tropic HIV and this correlates in part with co-receptor expression at the mRNA level.

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1998-01-01
2022-05-19
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