1887

Abstract

The recent isolation of GB viruses A and B from GB agent infected tamarins and their lack of involvement in human hepatitis has sparked interest in the origin of these viruses. Several healthy non-human primate species have been shown to harbour sequences 52–79% identical to the GBV-A 5′ nontranslated region. In this paper we report the near genome length sequence of GBV-A 70047 and GBV-A, 1122. These sequences support previous observations about the genomic organization of GBV-A and provide insight into the genomic variability within this virus genus. Although the GBV-A variant polyproteins possess many motifs conserved between other members of the , they do not encode a basic core-like protein. Amino acid sequence comparisons and phylogenetic analysis demonstrate variability within the GBV-A genus similar to that observed between hepatitis C virus (HCV) types. However, genomic organization and disease association demonstrate a closer evolutionary relationship to GBV-C than to HCV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-1-41
1998-01-01
2023-02-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/1/9460920.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-1-41&mimeType=html&fmt=ahah

References

  1. Bukh J., Apgar C. L. 1997; Five new or recently discovered (GBV-A) virus species are indigenous to new world monkeys and may constitute a separate genus of the Flaviviridae. Virology 229:429–436
    [Google Scholar]
  2. Colombatto P., Randone A., Civitico G., Monti Gorin J., Dolci L., Medaina N., Oliveri F., Verme G., Marchiaro G., Pagni R., Karayiannis P., Thomas H. C., Hess G., Bonino F., Brunetto M. R. 1996; Hepatitis G virus RNA in the serum of patients with elevated gamma glutamyltranspeptidase and alkaline phosphatase: a specific liver disease. Journal of Viral Hepatitis 3:301–306
    [Google Scholar]
  3. Erker J. C., Simons J. N., Muerhoff A. S., Leary T. P., Chalmers M. L., Desai S. M., Mushahwar I. K. 1996; Molecular cloning and characterization of a GB virus C isolate from a patient with non-A-E hepatitis. Journal of General Virology 77:2713–2720
    [Google Scholar]
  4. Fiordalisi G., Zanella I., Mantero G., Bettinardi A., Stellini R., Paraninfo G., Cadeo G., Primi D. 1996; High prevalence of GB virus C infection in a group of Italian patients with hepatitis of unknown etiology. Journal of Infectious Diseases 174:181–183
    [Google Scholar]
  5. Grakoui A., McCourt D. W., Wychowski C., Feinstone S. M., Rice C. M. 1993a; A second hepatitis C virus-encoded proteinase. Proceedings of the National Academy of Sciences, USA 90:10583–10587
    [Google Scholar]
  6. Grakoui A., McCourt D. W., Wychowski C., Feinstone S. M., Rice C. M. 1993b; Characterization of the hepatitis C virus-encoded serine proteinase: determination of protease-dependent polyprotein cleavage sites. Journal of Virology 67:2832–2843
    [Google Scholar]
  7. Leary T. P., Desai S. M., Yamaguchi J., Chalmers M. L., Schlauder G. G., Dawson G. J., Mushahwar I. K. 1996a; Species-specific variants of GB virus A in captive monkeys. Journal of Virology 70:9028–9030
    [Google Scholar]
  8. Leary T. P., Muerhoff A. S., Simons J. N., Pilot-Matias T. J., Erker J. C., Chalmers M. L., Schlauder G. G., Dawson G. J., Desai S. M., Mushahwar I. K. 1996b; The sequence and genomic organization of GBV-C: a novel member of the Flaviviridae associated with human non-A-E hepatitis. Journal of Medical Virology 48:60–67
    [Google Scholar]
  9. Leary T. P., Desai S. M., Erker J. C., Mushahwar I. K. 1997; The sequence and genomic organization of a GB virus A variant isolated from captive tamarins. Journal of General Virology 78:2307–2313
    [Google Scholar]
  10. Muerhoff A. S., Leary T. P., Simons J. N., Pilot-Matias T. P., Erker J. C., Chalmers M. L., Schlauder G. G., Dawson G. J., Desai S. M., Mushahwar I. K. 1995; Genomic organization of GBV-A and GBV-B: two new members of the Flaviviridae associated with GB-agent hepatitis. Journal of Virology 69:5621–5630
    [Google Scholar]
  11. Pilot-Matias T. J., Muerhoff A. S., Simons J. N., Leary T. P., Buijk S. L., Chalmers M. L., Erker J. C., Dawson G. J., Desai S. M., Mushahwar I. K. 1996a; Identification of antigenic regions in the GB hepatitis viruses GBV-A, GBV-B and GBV-C. Journal of Medical Virology 48:329–338
    [Google Scholar]
  12. Pilot-Matias T. J., Carrick R. J., Coleman P. F., Leary T. P., Surowy T. K., Simons J. N., Muerhoff A. S., Buijk S. L., Chalmers M. L., Dawson G. J., Desai S. M., Mushahwar I. K. 1996b; Expression of the GB virus C E2 glycoprotein using the Semliki Forest virus vector system and its utility as a serologic marker. Virology 225:282–292
    [Google Scholar]
  13. Roux K. H. 1995; Using mismatched primer-template pairs in touchdown PCR. Bio Techniques 16:812–814
    [Google Scholar]
  14. Schlauder G. G., Dawson G. J., Simons J. N., Pilot-Matias T. J., Guiterrez R. A., Heynen C. A., Knigge M. F., Kurpiewski G. S., Buijk S. L., Leary T. P., Muerhoff A. S., Desai S. M., Mushahwar I. K. 1995a; Molecular and serologic analysis in the transmission of the GB hepatitis agents. Journal of Medical Virology 46:81–90
    [Google Scholar]
  15. Schlauder G. G., Pilot-Matias T. J., Gabriel G. S., Simons J. N., Muerhoff A. S., Dawson G. J., Mushahwar I. K. 1995b; Origin of GB-hepatitis viruses. Lancet 346:447
    [Google Scholar]
  16. Simons J. N., Pilot-Matias T. J., Leary T. P., Dawson G. J., Desai S. M., Schlauder G. G., Muerhoff A. S., Erker J. C., Buijk S. L., Chalmers M. L., Van Sant C. L., Mushahwar I. K. 1995a; Identification of two flavivirus-like genomes in the GB hepatitis agent. Proceedings National Academy of Sciences, USA 92:3401–3405
    [Google Scholar]
  17. Simons J. N., Leary T. P., Dawson G. J., Pilot-Matias T. J., Muerhoff A. S., Schlauder G. G., Desai S. M., Mushahwar I. K. 1995b; Isolation of novel virus-like sequences associated with human hepatitis. Nature Medicine 1:564–569
    [Google Scholar]
  18. Simons J. N., Desai S. M., Schultz D. E., Lemon S. M., Mushahwar I. K. 1996; Translation initiation in GB viruses A and C: evidence for internal ribosome entry and implications for genome organization. Journal of Virology 70:6126–6135
    [Google Scholar]
  19. Simmonds P. 1995; Variability of hepatitis C virus. Hepatology 21:570–583
    [Google Scholar]
  20. Sorenson A. B., Duch M., Jorgensen P., Pedersen F. S. 1993; Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method. Journal of Virology 67:7118–7124
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-1-41
Loading
/content/journal/jgv/10.1099/0022-1317-79-1-41
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error