1887

Abstract

The interactions of seven isolates of peanut stunt cucumovirus (PSV) originating from North America, Europe and Africa, and two variants of PSV satellite RNA (sat RNA) were analysed. Electrophoretic and immunoblot analyses of the coat protein (CP) and Northern blot hybridization analyses of the viral RNAs showed that isolates PSV F352, 1339 and 1507 belonged to subgroup I, and isolates PSV W, Su and B to subgroup II. The seventh isolate, robinia mosaic virus (RoMV) clustered with subgroup I isolates by CP analysis, but was related to both subgroups by RNA hybridization analysis. The ability to support the accumulation of two newly described sat RNA variants, P4 and P6 sat RNAs, was not related to PSV isolate classification: neither PSV W nor RoMV were helper viruses for these PSV sat RNAs. Symptom modulation by both sat RNAs was the same: the presence of sat RNA did not modify the symptoms induced by subgroup I isolates but exacerbated the symptoms induced by subgroup II isolates in both tobacco and cowpea. Sat RNAs P4 and P6 contained 393 nucleotides, and differed only in three nucleotide substitutions. This resulted in marked differences in infectivity, level of accumulation and relative encapsidation between both the sat RNAs. Accumulation levels and relative encapsidation of sat RNAs was also affected by the isolate of helper virus.

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1998-01-01
2024-04-19
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References

  1. Collmer C. W., Hadidi A., Kaper J. M. 1985; Nucleotide sequence of the satellite of peanut stunt virus reveals structural homologies with viroids and certain nuclear and mitochrondial introns. Proceedings of the National Academy of Sciences, USA 82:3110–3114
    [Google Scholar]
  2. Dlaz-Ruiz J. R., Kaper J. M. 1978; Isolation of viral double-stranded RNAs using a LiCl fractionation procedure. Preparative Biochemistry 8:1–17
    [Google Scholar]
  3. Dlaz-Ruiz J. R., Kaper J. M. 1983; Nucleotide sequence relationships among thirty peanut stunt virus isolates determined by competition hybridization. Archives of Virology 75:277–281
    [Google Scholar]
  4. Dlaz-Ruiz J. R., Kaper J. M., Waterworth H. E., Devergne J. C. 1979; Isolation and characterization of peanut stunt virus from alfalfa in Spain. Phytopathology 69:504–509
    [Google Scholar]
  5. Ding S. W., Anderson B. J., Haase H. R., Symons R. H. 1994; New overlapping gene encoded by the cucumber mosaic virus genome. Virology 198:593–601
    [Google Scholar]
  6. García-Arenal F., Zaitlin M., Palukaitis P. 1987; Sequence analysis of six satellite RNAs of cucumber mosaic virus differing in their pathology. Virology 158:339–347
    [Google Scholar]
  7. Ghabrial S. A., Pickard C. M., Stucki R. E. 1977; Identification and distribution of virus diseases of soybean in Kentucky. Plant Disease Reporter 61:690–694
    [Google Scholar]
  8. Ferreiro C., Ostrówka K., López-Moya J. J., Díaz-Ruiz J. R. 1996; Nucleotide sequence and symptom modulating analysis of a peanut stunt virus-associated satellite RNA from Poland: high level of sequence identities with the American PSV satellites. European Journal of Plant Pathology 102:779–786
    [Google Scholar]
  9. Fraile A., García-Arenal F. 1991; Secondary structure as a constraint to the evolution of a plant viral satellite RNA. Journal of Molecular Biology 221:1065–1069
    [Google Scholar]
  10. Hu C.-C., Aboul-Ata A. E., Naidu R. A., Ghabrial S. A. 1997; Evidence for the occurrence of two distinct subgroups of peanut stunt cucumovirus strains: molecular characterization of RNA3. Journal of General Virology 78:929–939
    [Google Scholar]
  11. Kaper J. M., Tousignant M. E., Díaz-Ruiz J. R., Tolin S. A. 1978; Peanut stunt virus-associated RNA 5 : second tripartite genome virus with an associated satellite-like replicating RNA. Virology 88:166–170
    [Google Scholar]
  12. Karasawa A., Kazuhiro N., Kakutani T., Minobe Y., Ehara Y. 1991; Nucleotide sequence of RNA 3 of peanut stunt virus cucumovirus. Virology 185:464–467
    [Google Scholar]
  13. Karasawa A., Kazuhiro N., Kakutani T., Minobe Y., Ehara Y. 1992; Nucleotide sequence analyses of peanut stunt cucumovirus RNAs 1 and 2. Journal of General Virology 73:701–707
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  15. McGarvey P., Tousignant M., Geletka L., Cellini F., Kaper J. M. 1995; The complete sequence of a cucumber mosaic virus from Ixora that is deficient in the replication of satellite RNAs. Journal of General Virology 76:2257–2270
    [Google Scholar]
  16. Mink G. I. 1972; Peanut stunt virus. CMI/AAB Descriptions of Plant Viruses92
    [Google Scholar]
  17. Mink G. I., Hebert T. T., Silbernagel M. J. 1967; A strain of peanut stunt virus isolated from beans in Washington. (Abstr.). Phytopathology 57:1400
    [Google Scholar]
  18. Mink G. I., Silbernagel M. J., Saksena K. N. 1969; Host range, purification, and properties of the Western strain of peanut stunt virus. Phytopathology 59:1625–1631
    [Google Scholar]
  19. Moriones E., Roossinck M. J., García-Arenal F. 1991; Nucleotide sequence of tomato aspermy virus RNA 2. Journal of General Virology 72:779–783
    [Google Scholar]
  20. Naidu R. A., Collins G. B., Ghabrial S. A. 1991; Symptom-modulating properties of peanut stunt virus satellite RNA sequence variants. Molecular Plant-Microbe Interactions 4:268–275
    [Google Scholar]
  21. Naidu R. A., Hu C.-C., Pennington R. E., Ghabrial S. A. 1995; Differentiation of Eastern and Western strains of peanut stunt cucumo-virus based on satellite RNA support and nucleotide sequence homology. Phytopathology 85:502–507
    [Google Scholar]
  22. Owen J., Palukaitis P. 1988; Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Virology 166:495–502
    [Google Scholar]
  23. Palukaitis P., Zaitlin M. 1984; Satellite RNAs of cucumber mosaic virus: characterization of two new satellites. Virology 132:426–435
    [Google Scholar]
  24. Palukaitis P., Roossinck M. J., Dietzgen R. G., Francki R. I. B. 1992; Cucumber mosaic virus. Advances in Virus Research 41:281–348
    [Google Scholar]
  25. Richter J., Haack M., Wesemann M., Beczner L. 1987; Differentiation of peanut stunt virus isolates in six serotypes. ArchivfuerPhytopathologie und Pflanzenschutz 2:127–133
    [Google Scholar]
  26. Roossinck M. J., Sleat D., Palukaitis P. 1992; Satellite RNAs of plant viruses: structures and biological effects. MicrobiologicalReviews 56:265–279
    [Google Scholar]
  27. Sadiq E. O., Ahmed A. H. 1985; Comparative studies on aphid transmission of the Sudan strain of peanut stunt virus. Phytopathologische Zeitschrift 115:160–164
    [Google Scholar]
  28. Saiki R. H., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. 1988; Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
    [Google Scholar]
  29. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  31. Schmelzer K. 1967; Zur Kenntnis des Echten Robinienmosaik-Virus. Phytopathologische Zeitschrift 58:59–86
    [Google Scholar]
  32. Schmelzer K. 1971; Robinia mosaic virus. CMI/AAB Descriptions of plant viruses65
    [Google Scholar]
  33. Sleat D. E., Palukaitis P. 1990; Induction of tobacco chlorosis by certain cucumber mosaic virus satellite RNAs is specific to subgroup II helper strains. Virology 176:292–295
    [Google Scholar]
  34. Xu Z., Barnett O. W., Gibson P. B. 1986; Characterization of peanut stunt virus strains by host reaction, serology, and RNA patterns. Phytopathology 76:390–395
    [Google Scholar]
  35. Zelcer A., Weaber K. F., Balazs E., Zaitlin M. 1981; The detection and characterization of viral-related double-stranded RNAs in tobacco mosaic virus infected plants. Virology 113:417–427
    [Google Scholar]
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