1887

Abstract

Potato plants carrying the Ry gene from are extremely resistant to a number of potyviruses, but it is not known at what stage of infection the resistance is expressed. The resistance maybe due to Ry or to a closely linked gene. In this investigation, we used potato virus Y (PVY) and a tobacco etch virus construct that encodes -glucuronidase (TEV-GUS) to monitor virus infections of potato plants. Systemic spread of either virus in resistant potato plants was not detectable by serology, RT-PCR, GUS assay or bioassay although each replicated in the initially infected cells of leaves from resistant potato cultivars and was transported into neighbouring cells. However, 3 days post-inoculation (p.i.) a necrotic reaction set in that stopped movement and accumulation of both viruses by 7 days p.i. The resistance reaction (probably a hypersensitive reaction) became visible as necrotic streaks on veins on the lower leaflet surfaces of some potato cultivars carrying the Ry gene and may be elicited by a common potyviral gene product.

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1998-01-01
2022-05-23
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References

  1. Arroyo R., Soto M. J., Martínez-Zapater J. M., Ponz F. 1996; Impaired cell-to-cell movement of potato virus Y in pepper plants carrying the ya(pr21) resistance gene. Molecular Plant-Microbe Interactions 9:314–318
    [Google Scholar]
  2. Barker H. 1996; Inheritance of resistance to potato virus Y and A in progeny obtained from potato cultivars containing gene Ry: evidence for a new gene for extreme resistance to PVA. Theoretical and Applied Genetics 93:710–716
    [Google Scholar]
  3. Barker H., Harrison B. D. 1984; Expression of genes for resistance to potato virus Y in potato plants and protoplasts. Annals of Applied Biology 105:539–545
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Carrington J. C., Freed D. D. 1990; Cap-independent enhancement of translation by a plant potyvirus 5′ nontranslated region. Journal of Virology 64:1590–1597
    [Google Scholar]
  6. Chrzanowska M. 1995; The reaction of Polish potato cultivars to PVYNTN. Potato Research 38:434–435
    [Google Scholar]
  7. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  8. Cockerham G. 1970; Genetical studies on resistance to potato viruses X and Y. Heredity 25:309–348
    [Google Scholar]
  9. Dolja V. V., McBride H. J., Carrington J. C. 1992; Tagging of plant potyvirus replication and movement by insertion of β-glucuronidase into the viral polyprotein. Proceedings of the National Academy of Sciences, USA 89:10208–10212
    [Google Scholar]
  10. Dolnicar P. 1995; The segregation for extreme resistance in progeny infected with different isolates of PVYn strain. Potato Research 38:434
    [Google Scholar]
  11. Fernandez-Northcote E. N. 1983; Prospects for stability ofresistance to potato virus Y. In Research for the Potato in the Year 2000. Proceedings of the International Congress in Celebration of the Tenth Anniversary of the International Potato Center, Lima, Peru22-27 February 198282 Hooker W. J. Edited by Peru: International Potato Center (CIP);
    [Google Scholar]
  12. Flis B. 1995; Inheritance of extreme resistance to potato virus Y in potato. Potato Research 38:199–210
    [Google Scholar]
  13. Gibb K. S., Hellmann G. M., Pirone T. P. 1989; Nature of resistance of a tobacco cultivar to tobacco vein mottling virus. Molecular Plant-Microbe Interactions 2:332–339
    [Google Scholar]
  14. Hinrichs J., Berger S., Buchenauer H. 1995; Extreme resistance to potato virus Y based on the Ry-gene is not expressed in potato protoplasts. Zeitschrift für Pflanzenkrankheiten and Pflanzenschutz 102:243–248
    [Google Scholar]
  15. Hinrichs J., Berger S., Buchenauer H. 1997; Susceptibility of potato cultivars to the N and O strain of potato virus Y (PVY) and other potyviruses. Zeitschrift für Pflanzenkrankheiten and Pflanzenschutz 104:193–199
    [Google Scholar]
  16. Jefferson R. A. 1987; Assaying chimeric genes in plants: the GUS gene fusion system. Plant Molecular Biology Reporter 5:387–405
    [Google Scholar]
  17. Jones R. A. C. 1990; Strain group specific and virus specific hypersensitive reactions to infection with potyviruses in potato cultivars. Annals of Applied Biology 117:93–105
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  19. Langeveld S. A., Dore J.-M., Memelink J., Derks A. F. L. M., van der Vlugt C. I. M., Asjes C. J., Bol J. F. 1991; Identication of potyviruses using the polymerase chain reaction with degenerate primers. Journal of General Virology 72:1531–1541
    [Google Scholar]
  20. Le Romancer M., Nedellec M. 1997; Effect of plant genotype, virus isolate and temperature on the expression of the potato tuber necrotic ringspot disease (PTNRD). Plant Pathology 46:104–111
    [Google Scholar]
  21. Luciano C. S., Gibb K. S., Berger P. H. 1989; A general method for the detection of potyviral gene products in plant protoplasts and tissue. Journal of Virological Methods 24:347–356
    [Google Scholar]
  22. Robaglia C., Durand-Tardif M., Tronchet M., Boudazin G., Astier-Manifacier S., Casse-Delbart F. 1989; Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology 70:935–947
    [Google Scholar]
  23. Ross H. 1986; Potato breeding-problems and perspectives. Advances in Plant Breeding13
    [Google Scholar]
  24. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Schaad M. C., Carrington J. C. 1996; Suppression of long-distance movement of tobacco etch virus in a nonsusceptible host. Journal of Virology 70:2556–2561
    [Google Scholar]
  26. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets : procedure and some applications. Proceedings of the National Academy of Sciences, USA 76:4350–4354
    [Google Scholar]
  27. Valkonen J. P. T. 1994; Natural genes and mechanisms for resistance to viruses in cultivated and wild potato species (Solanum spp.). Plant Breeding 112:1–16
    [Google Scholar]
  28. Valkonen J. P. T., Somersalo S. 1996; Patterns and barriers of cell-to-cell movement and lack of systemic spread of tobacco etch potyvirus (TEV-GUS) in Solanum brevidens. Plant Science 113:221–228
    [Google Scholar]
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