1887

Abstract

The complete nucleotide sequence of the cDNA genome for garlic virus X (GVX), one of the major viruses infecting garlic plants, was determined. GVX is a single-stranded positive-sense RNA virus consisting of 8106 nucleotides excluding the 3 ′-end poly(A) tail and contains six open reading frames (ORFs) which encode putative proteins of 174 kDa (ORF1), 26 kDa (ORF2), 12 kDa (ORF3), 32 kDa (ORF4), 26 kDa (ORF5) and 15 kDa (ORF6). The putative viral proteins show similarity to those of carlaviruses and potexviruses but show the highest homology to shallot virus X (ShVX). Even though the GVX genome contains most of the structural elements common to carlaviruses and potexviruses, it is distinguished from them by the presence of an ORF4 which encodes an unusual protein. These results suggest that GVX may belong to an unassigned group of ShVX and GarV-type viruses rather than to the carlaviruses or potexviruses.

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1998-01-01
2024-04-23
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References

  1. Ahlawat Y. S. 1974; A mosaic disease of garlic in Darjeeling hills. Science and Culture 40:466–467
    [Google Scholar]
  2. Bancroft J. B., Rouleau M., Johnston R., Prins L., Mackie G. A. 1991; The complete nucleotide sequence of foxtail mosaic virus RNA. Journal of General Virology 71:2173–2181
    [Google Scholar]
  3. Beck D. L., Guilford P. J., Voot D. M., Andersen M. T., Forster R. L. S. 1991; Triple gene block proteins of white clover mosaic potexvirus are required for transport. Virology 183:695–702
    [Google Scholar]
  4. Chang M. U., Park W. W., Chung J. D., Lim K. B., La Y. J. 1988; Distribution of garlic latent virus and garlic mosaic virus in infected garlic tissues. Journal of the Korean Society for Horticultural Science 29:253–265
    [Google Scholar]
  5. Choi J. N., Song J. T., Song S. I., Ahn J. H., Choi Y. D., Lee J. S. 1995; Immunological detection of garlic latent virus. Agricultural Chemistry and Biotechnology 38:49–54
    [Google Scholar]
  6. Forster R. L. S., Bevan M. W., Harbison S.-A., Gardner R. C. 1988; The complete nucleotide sequence of the potexvirus white clover mosaic virus. Nucleic Acids Research 16:291–303
    [Google Scholar]
  7. Foster G. D. 1992; The structure and expression of the genome of carlaviruses. Research in Virology 143:103–112
    [Google Scholar]
  8. Foster G. D., Mills P. R. 1991; Nucleotide sequence of the 7K gene of carnation latent virus. Plant Molecular Biology 15:937–939
    [Google Scholar]
  9. Gordon K., Fuetterer J., Hohn T. 1992; Efficient initiation of translation at non-AUG triplets in plant cells. Plant Journal 2:809–813
    [Google Scholar]
  10. Gramstat A., Courtpozanis A., Rohde W. 1990; The 12 kDa protein of potato virus M displays properties of a nucleic acid-binding regulatory protein. FEBS Letters 276:34–38
    [Google Scholar]
  11. Huisman M. J., Linthorst H. J. M., Bol J. F., Cornelissen B. J. C. 1988; The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. Journal of General Virology 69:1789–1798
    [Google Scholar]
  12. Jelkmann W. E., Maiss E., Martin R. R. 1992; The nucleotide sequence and organization of strawberry mild edge-associated potex-virus. Journal of General Virology 73:475–479
    [Google Scholar]
  13. Kanyuka K. V., Vishnichenko V. K., Levay K. E., Kondrikov D. Y., Ryabov E. V., Zavriev S. K. 1992; Nucleotide sequence of shallot virus X RNA reveals a 5′-proximal cistron closely related to those of potexviruses and a unique arrangement of the 3′-proximal cistrons. Journal of General Virology 73:2553–2560
    [Google Scholar]
  14. Kobayashi K., Rabinowicz P., Bravoalmonacid F., Helguera M., Conci V., Lot H., Mentaberry A. 1996; Coat protein gene sequences of garlic and onion isolates of the onion yellow dwarf potyvirus (OYDV). Archives of Virology 141:2277–2287
    [Google Scholar]
  15. Lee Y. W., Yamazaki S., Osaki T., Inouye T. 1979; Two elongated viruses in garlic, garlic latent virus and garlic mosaic virus. Annals of the Phytopathological Society of Japan 45:727–734
    [Google Scholar]
  16. Levay K., Zavriev S. 1991; Nucleotide sequence and gene organization of the 3′-terminal region of chrysanthemum virus B genomic RNA. Journal of General Virology 72:2333–2337
    [Google Scholar]
  17. Mackenzie D. J., Tremaine J. H., Stace-Smith R. 1989; Organization and interviral homologies of the 3′-terminal portion of potato virus S RNA. Journal of General Virology 70:1053–1063
    [Google Scholar]
  18. Marys E., Carballo O., Izagurirre-Mayoral M. L. 1994; Isolation and characterization of viruses present in four clones of garlic (Allium sativum) in Venezuela. Journal of Phytopathology 142:227–234
    [Google Scholar]
  19. Memelink J., van der Vlugt C. I. M., Linthorst H. J. M., Derks A. F. L. M., Asjes C. J., Bol J. F. 1990; Homologies between the genomes of a carlavirus (lily symptomless virus) and a potexvirus (lily virus X) from lily plants. Journal of General Virology 71:917–924
    [Google Scholar]
  20. Nagakubo T., Kubo M., Oeda K. 1994; Nucleotide sequence of the 3′ regions of two major viruses from mosaic-diseased garlic: molecular evidence of mixed infection by a potyvirus and a carlavirus. Phytopathology 84:640–645
    [Google Scholar]
  21. Petty I. T. D., Jackson A. O. 1990; Two forms of the major barley stripe mosaic virus nonstructural protein are synthesized in vivo from alternative initiation codons. Virology 177:829–832
    [Google Scholar]
  22. Ryabov E. V., Generozov E. V., Vetten D. J., Zavriev S. K. 1996; Nucleotide sequence of the 3′-terminal region of mite-borne filamentous virus indicates its relation to the shallot virus X group. Molecular Biology 30:63–67
    [Google Scholar]
  23. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  24. Song J. T., Choi J. N., Song S. I., Lee J. S., Choi Y. D. 1995; Identification of a potexvirus in Korean garlic plants. Agricultural Chemistry and Biotechnology 38:55–62
    [Google Scholar]
  25. Sumi S., Tsuneyoshi T., Furutani H. 1993; Novel rod-shaped viruses isolated from garlic, Allium sativum, possessing a unique genome organization. Journal of General Virology 74:1879–1885
    [Google Scholar]
  26. Tsuneyoshi T., Sumi S. 1996; Differentiation among garlic viruses in mixed infections based on RT-PCR procedures and direct tissue blotting immunoassays. Phytopathology 86:253–259
    [Google Scholar]
  27. Van Dijk P. 1993; Survey and characterization of potyviruses and their strains of Allium species. Netherlands Journal of Plant Pathology 99: suppl. 2 1–48
    [Google Scholar]
  28. Yamashita K., Sakai J., Hanada K. 1996; Characterization of a new virus from garlic (Allium sativum L.), garlic mite borne mosaic virus. Annals of the Phytopathological Society of Japan 62:483–489
    [Google Scholar]
  29. Zavriev S. K., Kanyuka K. V., Levay K. E. 1991; The genome organization of potato virus M RNA. Journal of General Virology 72:9–14
    [Google Scholar]
  30. Zimmern D. 1987; Evolution of RNA viruses. In RNA Genetics pp. 211–240 Holland J., Domingo E., Ahlquist P. Edited by Boca Raton, Fla: CRC Press;
    [Google Scholar]
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