1887

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Volume 79, Issue 1

Characterization of the bovine herpesvirus 1 UL8 gene and gene products Free

Abstract

The bovine herpesvirus 1 (BHV-1) strain Schön-böken UL8 gene and the 5′ flanking region were sequenced. Comparison of the UL8 ORF with the previously reported UL8 ORF of BHV-1 strain Cooper revealed significant differences that were mainly due to three frame-shifted segments. Reanalysis of the Cooper sequence after isolation of the respective segments from genomic DNA by PCR did not confirm the discrepancies; on the contrary, our results indicate a high degree of sequence conservation between the UL8 proteins of different BHV-1 isolates. A monospecific antiserum, raised against a bacterially expressed TrpE-UL8 fusion protein, identified the 80 kDa apparent molecular mass UL8 polypeptide which is localized in the nucleus of infected cells. Analysis of transcripts and time-course studies demonstrated that the UL8 protein is translated from a delayed-early expressed 3·1 kb polyadenylated mRNA which initiates within the UL9 ORF.

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© Society for General Microbiology 1998
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1998-01-01
2024-04-19
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Characterization of the bovine herpesvirus 1 UL8 gene and gene products
J. Gen. Virol. 79, 133 (1998); https://doi.org/10.1099/0022-1317-79-1-133
/content/journal/jgv/10.1099/0022-1317-79-1-133
/content/journal/jgv/10.1099/0022-1317-79-1-133
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