1887

Abstract

Due to their simplicity and flexibility of genomic construction, herpes simplex virus (HSV) amplicon- based vectors are attractive vehicles for gene delivery. However, a significant problem faced in the generation of amplicon stocks is the low amplicon to helper virus (A/H) ratio. In order to improve the proportion of amplicons generated, a selection system for amplicon production was developed in which the HSVthymidine kinase (TK) gene is inserted into an amplicon plasmid and an HSV mutant with both TK and glycoprotein H (gH) genes deleted is used as a helper virus. Using a protocol in which amplicon stocks are passaged 2–3 times in BHK cells of TK and gH genotype in the presence of selection medium containing methotrexate, stock preparations with high A/H ratio (up to 5:1) and high amplicon titre (> 1 × 10 infectious units/ml) were generated. characterization demonstrated that a high level of biologically functional products can be efficiently produced from these amplicon constructs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-79-1-125
1998-01-01
2022-05-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/79/1/9460933.html?itemId=/content/journal/jgv/10.1099/0022-1317-79-1-125&mimeType=html&fmt=ahah

References

  1. Boothman D. A., Geller A. I., Pardee A. B. 1989; Expression of the E. coli lacZ gene from a defective HSV-1 vector in various human normal, cancer-prone and tumor cells. FEBS Letters 258:159–162
    [Google Scholar]
  2. Boursnell M. E. G., Entwisle C., Blakeley D., Roberts C., Duncan I. A., Chisholm S. E., Martin G. M., Jennings R., Ní Challanáin D., Sobek I., Inglis S. C., McLean C. S. 1997; A genetically inactivated herpes simplex virus type 2 (HSV-2) vaccine provides effective protection against primary and recurrent HSV-2 disease. Journal of Infectious Diseases 175:16–25
    [Google Scholar]
  3. Breakefield X. O., DeLuca N. A. 1991; Herpes simplex virus for gene delivery to neurons. New Biologist 3:203–218
    [Google Scholar]
  4. Desai P. J., Schaffer P. A., Minson A. C. 1988; Excretion of noninfectious virus particles lacking glycoprotein H by a temperature- sensitive mutant of herpes simplex virus type 1 : evidence that gH is essential for virion infectivity. Journal of General Virology 69:1147–1156
    [Google Scholar]
  5. During M. J., Naegele J. R., O’Malley K. L., Geller A. I. 1994; Long-term behavioral recovery in Parkinsonian rats by an HSV vector expressing tyrosine hydroxylase. Science 266:1399–1403
    [Google Scholar]
  6. Forrester A., Farrell H., Wilkinson G., Kaye J., Davis-Poynter N., Minson A. C. 1992; Construction and properties of a mutant of herpes simplex virus type 1 with glycoprotein H coding sequences deleted. Journal of Virology 66:341–348
    [Google Scholar]
  7. Fraefel C., Song S., Lim F., Lang P., Yu L., Wang Y., Wild P., Geller A. I. 1996; Help er virus-free transfer of herpes simplex virus type 1 plasmid vectors into neural cells. Journal of Virology 70:7190–7197
    [Google Scholar]
  8. Frenkel N. 1981; Defective interfering herpesviruses. In The Human Herpesviruses - an Interdisciplinary Perspective pp. 91–120 Nahmias A., Dowdle W., Schinazy R. Edited by New York: Elsevier-North Holland;
    [Google Scholar]
  9. Frenkel N. R. S., Vlazny D. A., Locker H. 1982; The herpes simplex virus amplicon - a novel animal-virus cloning vector. In Eukaryotic Viral Vectors pp. 205–209 Gluzman Y. Edited by Cold Spring Harbor, NY: Cold Spring Habor Laboratory;
    [Google Scholar]
  10. Geller A. I., During M. J., Haycock J. W., Freese A., Neve R. 1993; Long-term increases in neurotransmitter release from neuronal cells expressing a constitutively active adenylate cyclase from a herpes simplex virus type 1 vector. Proceedings of the National Academy of Sciences, USA 90:7603–7607
    [Google Scholar]
  11. Glorioso J. G., Goins W. F., Fink D. J. 1992; Herpes simplex virus-based vectors. Seminars in Virology 3:265–276
    [Google Scholar]
  12. Gompels U., Minson A. C. 1986; The properties and sequence of glycoprotein H of herpes simplex virus type 1. Virology 153:230–247
    [Google Scholar]
  13. Hill T. J., Field H. J., Blyth W. A. 1975; Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. Journal of General Virology 28:341–353
    [Google Scholar]
  14. Ho D. Y. 1994; Amplicon-based herpes simplex virus vectors. Methods in Cell Biology 43:191–210
    [Google Scholar]
  15. Kwong A. D., Frenkel N. 1984; Herpes simplex virus amplicon: effect of size on replication of constructed defective genomes containing eucaryotic DNA sequences. Journal of Virology 51:595–603
    [Google Scholar]
  16. Leib D. A., Olivo P. D. 1993; Gene delivery to neurons : is herpes simplex virus the right tool for the job?. Bioessays 15:547–554
    [Google Scholar]
  17. Pechan P. A., Fotaki M., Thompson R. L., Dunn R., Chase M., Chiocca E. A., Breakefield X. O. 1996; A novel ‘piggyback’ packaging system for herpes simplex virus amplicon vectors. Human Gene Therapy 7:2003–2013
    [Google Scholar]
  18. Roizman B. 1990; Herpes simplex viruses and their replication. In Virology, 2nd edition. pp. 1795–1840 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  19. Smith R. L., Geller A. I., Escudero K. W., Wilcox C. L. 1995; Longterm expression in sensory neurons in tissue culture from herpes simplex virus type 1 (HSV-1) promoters in an HSV-1-derived vector. Journal of Virology 69:4593–4599
    [Google Scholar]
  20. Spate R. R., Frenkel N. 1982; The herpes simplex virus amplicon: a new eucaryotic defective-virus cloning-amplifying vector. Cell 30:295–304
    [Google Scholar]
  21. Stow N. D. 1982; Localization of an origin of DNA replication within the TRs/IRs repeated region of the herpes simplex virus type 1 genome. EMBO Journal 1:863–867
    [Google Scholar]
  22. Stow N. D., McMonagle E. C., Davison A. J. 1983; Fragments from both termini of the herpes simplex virus type 1 genome contain signals required for the encapsidation of viral DNA. Nucleic Acids Research 11:8205–8220
    [Google Scholar]
  23. Vlazny D. A., Frenkel N. 1981; Replication of herpes simplex virus DNA: location of replication recognition signals within defective virus genomes. Proceedings of the National Academy of Sciences, USA 78:742–746
    [Google Scholar]
  24. Wu X., Leduc Y., Cynader M., Tufaro F. 1995; Examination of conditions affecting the efficiency of HSV-1 amplicon packaging. Journal of Virology Methods 52:219–229
    [Google Scholar]
  25. Zhang X., Efstathiou S., Simmons A. 1994; Identification of novel herpes simplex virus replicative intermediates by field inversion gel electrophoresis: implications for viral DNA amplification strategies. Virology 202:530–539
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-79-1-125
Loading
/content/journal/jgv/10.1099/0022-1317-79-1-125
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error