1887

Abstract

Comparison of nonstructural glycoprotein NSP4 gene sequences from 22 rotavirus strains originating from six host species and of 14 different combinations of G and P types revealed the presence of three distinct NSP4 alleles, represented by strains Wa, KUN and AU-1. Genetic distances between any of these alleles (18·0%) were significantly greater than those within each allele (5·5%) and phylogenetic analysis suggested that divergence into three distinct alleles had occurred at about the same time during evolution. While amino acid variation among strains was minimal in the amino-terminal two-thirds of the protein (aa 1–130), variability increased toward the carboxy terminus of the enterotoxic peptide region (aa 114–135) and was greatest between residues 135 and 141. Comparison of the amino acid sequences corresponding to the enterotoxic peptide region between strains isolated from asymptomatic neonates and those from children with diarrhoea failed to identify any conserved changes that correlated with the capacity of the virus to cause disease. Amino acids were relatively conserved in the domains important for viral morphogenesis.

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1997-09-01
2024-04-18
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References

  1. Au K.-S., Mattion N. M., Estes M. K. 1993; A subviral particle binding domain on the rotavirus nonstructural glycoprotein NS28. Virology 194:665–673
    [Google Scholar]
  2. Ball J. M., Tian P., Zeng C. Q.-Y., Morris A. P., Estes M. K. 1996; Age-dependent diarrhea induced by a rotaviral nonstructural glycoprotein. Science 272:101–104
    [Google Scholar]
  3. Ballard A., McCrae M. A., Desselberger U. 1992; Nucleotide sequences of normal and rearranged RNA segments 10 of human rotaviruses. Journal of General Virology 73:633–638
    [Google Scholar]
  4. Baybutt H. N., McCrae M. A. 1984; The molecular biology of rotaviruses. VII. Detailed structural analysis of gene 10 of bovine rotavirus. Virus Research 1:533–541
    [Google Scholar]
  5. Bergmann C. C., Maass D., Poruchynsky M. S., Atkinson P. H., Bellamy A. R. 1989; Topology of the non-structural rotavirus receptor glycoprotein NS28 in the rough endoplasmic reticulum. EMBO Journal 8:1695–1703
    [Google Scholar]
  6. Both G. W., Siegman L. J., Bellamy A. R., Atkinson P. H. 1983; Coding assignment and nucleotide sequence of simian rotavirus SA11 gene segment 10: location of glycosylation sites suggests that the signal peptide is not cleaved. Journal of Virology 48:335–339
    [Google Scholar]
  7. Chan W.-K., Au K.-S., Estes M. K. 1988; Topography of the simian rotavirus nonstructural glycoprotein (NS28) in the endoplasmic reticulum membrane. Virology 164:435–442
    [Google Scholar]
  8. Dayhoff M. O., Schwartz R. M., Orcutt B. C. 1978; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure 5 suppl. 3 pp. 345–352 Dayhoff M. O. Edited by Silver Spring, Md: National Biomedical Research Foundation;
    [Google Scholar]
  9. Estes M. K. 1996; Rotaviruses and their replication. In Fields Virology, 3rd edn. pp. 1625–1655 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  10. Felsenstein J. 1993; PHYLIP (phylogeny inference package) version 3.5c. Distributed by the author. Department of Genetics, University of Washington, Seattle, Wash., USA:
    [Google Scholar]
  11. Kapikian A. Z., Chanock R. M. 1996; Rotaviruses. In Fields Virology, 3rd edn. pp. 1657–1708 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
    [Google Scholar]
  12. Kirkwood C. D., Coulson B. S., Bishop R. F. 1996; G3P2 rotaviruses causing diarrhoeal disease in neonates differ in VP4, VP7 and NSP4 sequence from G3P2 strains causing asymptomatic neonatal infection. Archives of Virology 141:1661–1676
    [Google Scholar]
  13. Lopez S., Arias C. F. 1993; Sequence analysis of rotavirus YM VP6 and NS28 proteins. Journal of General Virology 74:1223–1226
    [Google Scholar]
  14. Mattion N. M., Mitchell D. B., Both G. W., Estes M. K. 1991; Expression of rotavirus proteins encoded by alternative open reading frames of genome segment 11. Virology 181:295–304
    [Google Scholar]
  15. Nakagomi O., Nakagomi T. 1991; Genetic diversity and similarity among mammalian rotaviruses in relation to interspecies transmission of rotavirus. Archives of Virology 120:43–55
    [Google Scholar]
  16. Nakagomi O., Nakagomi T., Akatani K., Ikegami N. 1989; Identification of rotavirus genogroups by RNA-RNA hybridization. Molecular and Cellular Probes 3:251–261
    [Google Scholar]
  17. Nakagomi T., Ohshima A., Akatani K., Ikegami N., Katsushima N., Nakagomi O. 1990; Isolation and molecular characterization of a serotype 9 human rotavirus strain. Microbiology and Immunology 34:77–82
    [Google Scholar]
  18. Okada Y., Richardson M. A., Ikegami N., Nomoto A., Furuichi Y. 1984; Nucleotide sequence of human rotavirus genome segment 10, an RNA encoding a glycosylated virus protein. Journal of Virology 51:856–859
    [Google Scholar]
  19. Powell K. F. H., Gunn P. R., Bellamy A. R. 1988; Nucleotide sequence of bovine rotavirus genomic 10 : an RNA encoding the viral non-structural glycoprotein. Nucleic Acids Research 16:763
    [Google Scholar]
  20. Saitou N., Nei M. 1987; The neighbour-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4:406–425
    [Google Scholar]
  21. Taylor J. A., O’Brien J. A., Yeager M. 1996; The cytoplasmic tail of NSP4, the endoplasmic reticulum-localized non-structural glycoprotein of rotavirus, contains distinct virus binding and coiled coil domains. EMBO Journal 15:4469–4476
    [Google Scholar]
  22. Thompson J. D., Higgins D. G., Gibson T. J. 1994; CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Research 22:4673–4680
    [Google Scholar]
  23. Tian P., Ball J. M., Zeng C. Q.-Y., Estes M. K. 1996; The rotavirus nonstructural glycoprotein NSP4 possesses membrane destabilization activity. Journal of Virology 70:6973–6981
    [Google Scholar]
  24. Wu T. T., Kabat E. A. 1970; An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity. Journal of Experimental Medicine 132:211–250
    [Google Scholar]
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