Inhibition of influenza viral polymerases by minimal viral RNA decoys Free

Abstract

All gene segments of influenza virus share a common feature at their respective termini. Both the 5′- and 3′-terminal sequences are highly conserved and possess partial inverted complementarity. This allows for the formation of a double-stranded duplex, which plays a major role in transcription, replication and packaging of the viral genome. studies have shown that the viral polymerase binds to short RNA molecules containing these termini. In this study, attempts were made to test whether mini-RNA decoys containing either or both termini can inhibit the activity of the viral polymerase . RNA molecules containing either the 5′ or the 3′ noncoding sequences were unable to inhibit NS-CAT RNA replication, while mini-RNA decoys consisting of both the 5′ and 3′ noncoding sequences of vRNA or cRNA were able to efficiently inhibit the activity of the viral polymerases expressed from vaccinia virus vectors.

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/content/journal/jgv/10.1099/0022-1317-78-9-2329
1997-09-01
2024-03-29
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References

  1. Cianci C., Tiley L., Krystal M. 1995; Differential activation of the influenza virus polymerase via template RNA binding. Journal of Virology 69:3995–3999
    [Google Scholar]
  2. Desselberger U., Racaniello V. R., Zazra J. J., Palese P. 1980; The 3′ and 5′ end terminal sequences of influenza A, B, and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene 8:315–328
    [Google Scholar]
  3. Fodor E., Seong B. L., Brownlee G. G. 1993; Photochemical cross-linking of influenza A polymerase to its virion RNA promoter defines a polymerase binding site at residues 9 to 12 of the promoter. Journal of General Virology 74:1327–1333
    [Google Scholar]
  4. Fodor E., Pritlove D. C., Brownlee G. G. 1994; The influenza virus panhandle is involved in the initiation of transcription. Journal of Virology 68:4092–4096
    [Google Scholar]
  5. Hagen M., Chung T. D. Y., Butcher J. A., Krystal M. 1994; Recombinant influenza virus polymerase: requirement of both 5′ and 3′ viral ends for endonuclease activity. Journal of Virology 68:1509–1515
    [Google Scholar]
  6. Hsu M.-T., Parvin J. D., Gupta S., Krystal M., Palese P. 1987; Genomic RNAs of influenza viruses are held in a circular conformation in virions and in infected cells by a terminal panhandle. Proceedings of the National Academy of Sciences, USA 84:8140–8144
    [Google Scholar]
  7. Huang T.-S., Palese P., Krystal M. 1990; Determination of influenza virus proteins required for genome replication. Journal of Virology 64:5669–5673
    [Google Scholar]
  8. Krug R. M., Alonso-Caplen F. V., Julkunen I., Katz M. G. 1989; Expression and replication of the influenza virus genome. In The Influenza Viruses pp. 89–152 Krug R. M. Edited by New York: Plenum Press;
    [Google Scholar]
  9. Li X.-Q., Palese P. 1992; Mutational analysis of the promoter required for influenza virus virion RNA synthesis. Journal of Virology 66:4331–4338
    [Google Scholar]
  10. Luo G.-X., Palese P. 1992; Genetic analysis of influenza virus. Current Opinion in Genetics and Development 2:77–81
    [Google Scholar]
  11. Luo G.-X., Luytjes W., Enami M., Palese P. 1991; The polyadenylation signal of influenza virus RNA involves a stretch of uridines followed by the RNA duplex of the panhandle structure. Journal of Virology 65:2861–2867
    [Google Scholar]
  12. Luytjes W., Krystal M., Enami M., Parvin J. D., Palese P. 1989; Amplification, expression, and packaging of a foreign gene by influenza virus. Cell 59:1107–1113
    [Google Scholar]
  13. Mukaigawa J., Hatada E., Fukuda R., Shimizu K. 1991; Involvement of the influenza A virus PB2 protein in the regulation of viral gene expression. Journal of General Virology 72:2661–2670
    [Google Scholar]
  14. Nayak D. P., Chambers T. M., Akkina R. K. 1989; Structure of defective-interfering RNAs of influenza viruses and their role in interference. In The Influenza Viruses pp. 269–317 Krug R. M. Edited by New York: Plenum Press;
    [Google Scholar]
  15. Parvin J. D., Palese P., Honda A., Ishihama A., Krystal M. 1989; Promoter analysis of influenza virus RNA polymerase. Journal of Virology 63:5142–5152
    [Google Scholar]
  16. Pattnaik A. K., Ball L. A., LeGrone A. W., Wertz G. W. 1992; Infectious defective interfering particles of VSV from transcripts of a cDNA clone. Cell 69:1011–1020
    [Google Scholar]
  17. Perrotta A. T., Been M. D. 1991; A pseudoknot-like structure required for efficient self-cleavage of hepatitis delta virus RNA. Nature 350:434–436
    [Google Scholar]
  18. Piccone M. E., Fernandez-Sesma A., Palese P. 1993; Mutational analysis of the influenza virus vRNA promoter. Virus Research 28:99–112
    [Google Scholar]
  19. Robertson J. S. 1979; 5′ and 3′ terminal nucleotide sequences of the RNA genome segments of influenza virus. Nucleic Acids Research 6:3745–3757
    [Google Scholar]
  20. Seong B. L., Brownlee G. G. 1992a; A new method for reconstituting influenza polymerase and RNA in vitro and viral rescue in vivo. Virology 186:247–260
    [Google Scholar]
  21. Seong B. L., Brownlee G. G. 1992b; Nucleotides 9 to 11 of the influenza A virion RNA promoter are crucial for activity in vitro. Journal of General Virology 73:3115–3124
    [Google Scholar]
  22. Shih S. R., Nemeroff M. E., Krug R. M. 1995; The choice of alternative 5′ splice sites in influenza virus M1 mRNA is regulated by the viral polymerase complex. Proceedings of the National Academy of Sciences, USA 92:6324–6328
    [Google Scholar]
  23. Skehel J. J., Hay A. J. 1978; Nucleotide sequences at the 5′ termini of influenza virus RNAs and their transcripts. Nucleic Acids Research 5:1207–1219
    [Google Scholar]
  24. Sullenger B. A., Gallardo H. F., Ungers G. E., Gilboa E. 1990; Overexpression of TAR sequences renders cells resistant to human immunodeficiency virus replication. Cell 63:601–608
    [Google Scholar]
  25. Tiley L., Hagen M., Matthews J. T., Krystal M. 1994; Sequence-specific binding of the influenza virus RNA polymerase to sequences located at the 5′ ends of the viral RNAs. Journal of Virology 68:5108–5116
    [Google Scholar]
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