1887

Abstract

The genetic diversity of 32 Italian isolates of feline immunodeficiency virus (FIV) was studied. Isolates were obtained from domestic cats living in different areas. Sequence data were obtained from a 308 bp fragment of the p25 region of the gene. Phylogenetic relationships among these sequences and previously published sequences were determined. All the Italian isolates could be assigned to subtype B; however, four isolates formed two separate clusters and may represent genetic out- liers. The reliability of classification results was confirmed by repeating the phylogenetic analysis with DNA sequences from the entire genes of two isolates and from the surface glycoprotein domain of the gene of four isolates. It is concluded that the short segment of used permits reliable genotyping of FIV isolates. The study also shows that subtype B is largely prevalent in Italy.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-9-2247
1997-09-01
2022-05-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/9/9292012.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-9-2247&mimeType=html&fmt=ahah

References

  1. Bachmann M. H., Sodora D. L., Matthiason-Dubard M., Learn G. H., Rodrigo A. G., Mazzetti P., Hoover E. A., Mullins J. I. 1997; Genetic diversity of feline immunodeficiency virus: dual infection, recombination and distinct evolutionary rates between envelope sequence clades. Journal of Virology 71:4241–4253
    [Google Scholar]
  2. Bandecchi P., Matteucci D., Baldinotti F., Guidi G., Abramo F., Tozzini F., Bendinelli M. 1992; Prevalence of feline immunodeficiency virus and other retroviral infections in sick cats in Italy. Veterinary Immunology and Immunopathology 31:337–345
    [Google Scholar]
  3. Bendinelli M., Pistello M., Lombardi S., Poli A., Garzelli C., Matteucci D., Ceccherini-Nelli L., Malvaldi G., Tozzini F. 1995; Feline immunodeficiency virus : an interesting model for AIDS studies and an important cat pathogen. Clinical Microbiology Reviews 8:87–112
    [Google Scholar]
  4. Cammarota G., Da Prato L., Nicoletti E., Matteucci D., Bendinelli M., Pistello M. 1996; Quantitation of feline immunodeficiency proviruses in doubly infected cats using competitive PCR and a fluorescence-based RFLP. Journal of Virological Methods 62:21–31
    [Google Scholar]
  5. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791
    [Google Scholar]
  6. Felsenstein J. 1993; PHYLIP (phylogeny inference package). version 3.5c University of Washington, Seattle, Wash., USA:
    [Google Scholar]
  7. Giannecchini S., Matteucci D., Mazzetti P., Bendinelli M. 1996; Length of incubation time for feline immunodeficiency virus cultures. Journal of Clinical Microbiology 34:2036–2038
    [Google Scholar]
  8. Higgins D. G., Bleasby A. J., Fuchs R. 1992; CLUSTAL V: improved software for multiple sequence alignment. Computer Applications in the Biosciences 8:189–191
    [Google Scholar]
  9. Hilleman M. R. 1995; Whether and when an AIDS vaccine?. Nature Medicine 1:1126–1129
    [Google Scholar]
  10. Hosie M. J., Osborne R., Yamamoto J., Neil J. C., Jarrett O. 1995; Protection against homologous but not heterologous challenge induced by inactivated feline immunodeficiency virus vaccines. Journal of Virology 69:1253–1255
    [Google Scholar]
  11. Ishida T., Washizu T., Toriyabe K., Motoyashi S., Tomoda I., Pedersen N. C. 1989; Feline immunodeficiency virus in Japan. Journal of the American Veterinary Medical Association 194:221–225
    [Google Scholar]
  12. Johnson C. M., Torres B. A., Koyama H., Yamamoto J. K. 1994; FIV as a model for AIDS vaccination. AIDS Research and Human Retroviruses 10:225–228
    [Google Scholar]
  13. Kakinuma S., Motokawa K., Hohdatsu T., Yamamoto J. K., Koyama H., Hashimoto H. 1995; Nucleotide sequence of feline immunodeficiency virus : classification of Japanese isolates into two subtypes which are distinct from non Japanese subtypes. Journal of Virology 69:3639–3646
    [Google Scholar]
  14. Kimura M. 1980; A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16:111–120
    [Google Scholar]
  15. Kumar S., Tamura K., Nei M. 1993; MEGA:molecular evolutionary genetic analysis. version 1.01 Pennsylvania State University, State Park, Pa., USA:
    [Google Scholar]
  16. Learn G. R. Jr Korber B. T. M., Foley B., Hahn B. H., Wolinsky S. M., Mullins J. I. 1996; Maintaining the integrity of human immunodeficiency virus sequence databases. Journal of Virology 70:5720–5730
    [Google Scholar]
  17. Li W. H., Wu C. I., Luo C. C. 1985; A new method for estimating synonymous and nonsynonymous rates of nucleotide substitution considering the relative likelihood of nucleotide and codon changes. Molecular Biology and Evolution 2:96–99
    [Google Scholar]
  18. Matteucci D., Pistello M., Mazzetti P., Giannecchini S., Del Mauro D., Zaccaro L., Bandecchi P., Tozzini F., Bendinelli M. 1996; Vaccination protects against in vivo-grown feline immunodeficiency virus even in the absence of detectable neutralizing antibodies. Journal of Virology 70:617–622
    [Google Scholar]
  19. Miyazawa T., Fukasawa M., Hasegawa A., Maki N., Ikuta K., Takahashi E., Hayami M., Mikami T. 1991; Molecular cloning of a novel isolate of feline immunodeficiency virus biologically and genetically different from the original U.S. isolate. Journal of Virology 65:1572–1577
    [Google Scholar]
  20. Nei M., Gojobori T. 1986; Simple method for estimating the number of synonymous and nonsynonymous nucleotide substitutions. Molecular Biology and Evolution 3:418–426
    [Google Scholar]
  21. Olmsted R. A., Hirsch V. M., Purcell R. H., Johnson P. R. 1989; Nucleotide sequence analysis of feline immunodeficiency virus : genome organization and relationship to other lentiviruses. Proceedings of the National Academy of Sciences, USA 86:8088–8092
    [Google Scholar]
  22. Pancino G., Fossati I., Chappey C., Castelot S., Hurtrel B., Moraillon A., Klatzmann D., Sonigo P. 1993; Structure and variations of feline immunodeficiency virus envelope glycoproteins. Virology 192:659–662
    [Google Scholar]
  23. Pecoraro M. R., Tomonaga K., Miyazawa T., Kawaguchi Y., Sugita S., Tohya Y., Kai C., Etcheverrigaray M. E., Mikami T. 1996; Genetic diversity of Argentine isolates of feline immunodeficiency virus. Journal of General Virology 77:2031–2035
    [Google Scholar]
  24. Phillips T. R., Talbott R. L., Lamont C., Muir S., Lovelace K., Elder J. H. 1990; Comparison of two host cell range variants of feline immunodeficiency virus. Journal of Virology 64:4605–4613
    [Google Scholar]
  25. Poli A., Giannelli C., Pistello M., Zaccaro L., Pieracci M., Bendinelli M., Malvaldi G. 1992; Detection of salivary antibodies in cats infected with feline immunodeficiency virus. Journal of Clinical Microbiology 30:2038–2041
    [Google Scholar]
  26. Rigby M. A., Holmes E. C., Pistello M., Mackay A., Leigh Brown A. J., Neil J. C. 1993; Evolution of structural proteins of feline immunodeficiency virus : molecular epidemiology and evidence of selection for change. Journal of General Virology 74:425–436
    [Google Scholar]
  27. Robertson D. L., Hahn B. H., Sharp P. M. 1995; Recombination in AIDS viruses. Journal of Molecular Evolution 40:249–259
    [Google Scholar]
  28. Sabino E. C., Shpaer E. G., Morgado M. G., Korber B. T., Diaz R. S., Bongertz V., Cavalcante S., Galvao-Castro B., Mullins J. I., Mayer A. 1994; Identification of human immunodeficiency virus type 1 envelope genes recombinant between subtypes B and F in two epidemiologically linked individuals from Brazil. Journal of Virology 68:6340–6346
    [Google Scholar]
  29. Sodora D. L., Shpaer E. G., Kitchell B. E., Dow S. W., Hoover E. A., Mullins J. I. 1994; Identification of three feline immunodeficiency virus (FIV) env gene subtypes and comparison of the FIV and human immunodeficiency virus type 1 evolutionary pattern. Journal of Virology 68:2230–2238
    [Google Scholar]
  30. Stewart C.-B. 1993; The powers and pitfalls of parsimony. Nature 361:603–607
    [Google Scholar]
  31. Talbott R. L., Sparger E. E., Lovelace K. M., Fitch W. M., Pedersen N. C., Luciw P. A., Elder J. H. 1989; Nucleotide sequence and genomic organization of feline immunodeficiency virus. Proceedings of the National Academy of Sciences, USA 86:5743–5747
    [Google Scholar]
  32. Tozzini F., Matteucci D., Bandecchi F., Baldinotti F., Poli A., Pistello M., Siebelink K. H. L., Ceccherini-Nelli L., Bendinelli M. 1992; Simple in vitro methods for titrating feline immunodeficiency virus (FIV) and FIV neutralizing antibodies. Journal of Virological Methods 37:241–252
    [Google Scholar]
  33. Willett B. J., Jarrett O. editor 1995 Feline Immunology and Immunodeficiency Oxford: Oxford University Press;
    [Google Scholar]
  34. Yamamoto J. K., Okuda T., Ackley C. D., Louie H., Pembroke E., Zochlinski B., Munn R. J., Gardner M. B. 1991; Experimental vaccine protection against feline immunodeficiency virus. AIDS Research and Human Retroviruses 7:911–922
    [Google Scholar]
  35. Yamamoto J. K., Hohdatsu T., Olmsted R. A., Pu R., Louie H., Zochlinski B., Acevedo V., Johnson H. M., Soulds G. A., Gardner M. B. 1993; Experimental vaccine protection against homologous and heterologous strains of feline immunodeficiency virus. Journal of Virology 67:601–605
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-9-2247
Loading
/content/journal/jgv/10.1099/0022-1317-78-9-2247
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error