1887

Abstract

Glycoprotein M (gM) is one of the very few nonessential glycoproteins conserved throughout the herpesvirus family. Despite this conservation little is known about its function in virus replication. To test for the importance of gM in a natural virus-host system, 6-week-old piglets were intranasally infected with a gM mutant of the alphaherpesvirus pseudorabies virus (PrV). Following infection virus excretion from the nasal mucosa was decreased ca. 100-fold compared to wild-type or revertant virus. Clinical signs were limited to transiently elevated temperature. In contrast, animals infected by wild-type or revertant virus exhibited high fever, severe respiratory symptoms and affliction of the central nervous system. Prior infection with gM PrV conferred protection against challenge infection and animals mounted an antibody response against gM after wild-type virus infection. Thus, gM is important for efficient virus replication and deletion of gM may contribute to development of live attenuated, genetically marked vaccines.

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1997-09-01
2024-12-08
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References

  1. Baines J. D., Roizman B. 1991; The open reading frames UL3, UL4, UL10, and UL16 are dispensable for the replication of herpes simplex virus 1 in cell culture. Journal of Virology 65:938–944
    [Google Scholar]
  2. Baines J. D., Roizman B. 1993; The UL10 gene of herpes simplex virus 1 encodes a novel viral glycoprotein, gM, which is present in the virion and in the plasma membrane of infected cells. Journal of Virology 67:1441–1452
    [Google Scholar]
  3. Baskerville A., McFerran J. B., Dow C. 1973; Aujeszky’s disease in pigs. Veterinary Bulletin 43:465–480
    [Google Scholar]
  4. de Wind N., Wagenaar F., Pol J., Kimman T., Berns A. 1992; The pseudorabies virus homolog of the herpes simplex virus UL21 gene product is a capsid protein which is involved in capsid maturation. Journal of Virology 66:7096–7103
    [Google Scholar]
  5. de Wind N., Berns A., Gielkens A., Kimman T. 1993; Ribonucleotide reductase-deficient mutants of pseudorabies virus are avirulent for pigs and induce partial protective immunity. Journal of General Virology 74:351–359
    [Google Scholar]
  6. Dijkstra J. M., Visser N., Mettenleiter T. C., Klupp B. G. 1996; Identification and characterization of pseudorabies virus glycoprotein gM as a nonessential virion component. Journal of Virology 70:5684–5688
    [Google Scholar]
  7. Dijkstra J. M., Fuchs W., Mettenleiter T. C., Klupp B. G. 1997; Identification and transcriptional analysis of pseudorabies virus UL6 to UL12 genes. Archives of Virology 142:17–35
    [Google Scholar]
  8. Jöns A., Gerdts V., Lange E., Kaden V., Mettenleiter T. C. 1997; Attenuation of dUTPase-deficient pseudorabies virus for the natural host. Veterinary Microbiology in press
    [Google Scholar]
  9. Kaplan A. S., Vatter A. E. 1959; A comparison of herpes simplex and pseudorabies viruses. Virology 7:394–407
    [Google Scholar]
  10. Kari B., Li W., Cooper J., Goertz R., Radeke B. 1994; The human cytomegalovirus UL100 gene encodes the gC-II glycoproteins recognized by Group 2 monoclonal antibodies. Journal of General Virology 75:3081–3086
    [Google Scholar]
  11. Kimman T. G., de Wind N., Oei-Lie N., Pol J. M. A., Berns A. J. M., Gielkens A. L. J. 1992; Contribution of single genes within the unique short region of Aujeszky’s disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. Journal of General Virology 73:243–251
    [Google Scholar]
  12. Kit S., Kit M., Pirtle C. 1985; Attenuated properties of thymidine kinase-negative deletion mutants of pseudorabies virus. American Journal of Veterinary Research 46:1359–1367
    [Google Scholar]
  13. Klupp B. G., Lomniczi B., Visser N., Fuchs W., Mettenleiter T. C. 1995; Mutations affecting the UL21 gene contribute to avirulence of pseudorabies virus vaccine strain Bartha. Virology 212:466–473
    [Google Scholar]
  14. McGregor S., Easterday B. C., Kaplan A. S., Ben-Porat T. 1985; Vaccination of swine with thymidine kinase-deficient mutants of pseudorabies virus. American Journal of Veterinary Research 46:1494–1497
    [Google Scholar]
  15. MacLean C. A., Efstathiou S., Elliott M. L., Jamieson F. E., McGeoch D. J. 1991; Investigation of herpes simplex virus type 1 genes encoding multiply inserted membrane proteins. Journal of General Virology 72:897–906
    [Google Scholar]
  16. MacLean C. A., Robertson L. M., Jamieson F. E. 1993; Characterization of the UL10 gene product of herpes simplex virus type 1 and investigations of its role in vivo . Journal of General Virology 74:975–983
    [Google Scholar]
  17. Mettenleiter T. C. 1994; Pseudorabies (Aujeszky’s disease) virus: state of the art. Acta Veterinaria Hungarica 42:153–177
    [Google Scholar]
  18. Mettenleiter T. C. 1996; Immunobiology of pseudorabies (Aujeszky’s disease). Veterinary Immunology and Immunopathology 54:221–229
    [Google Scholar]
  19. Mettenleiter T. C., Rauh I. 1990; A glycoprotein gX-βgalactosidase fusion gene for rapid identification of pseudorabies virus mutants. Journal of Virological Methods 30:55–66
    [Google Scholar]
  20. Osterrieder N., Neubauer A., Brandmuller C., Braun B., Kaaden O.-R., Baines J. 1996; The equine herpesvirus 1 glycoprotein gp21/22a, the herpes simplex virus type 1 gM homolog, is involved in virus penetration and cell-to-cell spread of virions. Journal of Virology 70:4110–4115
    [Google Scholar]
  21. Pensaert M., Kluge J. 1989; Pseudorabies virus (Aujeszky’s disease). In Virus Infections of Porcines pp. 39–64 Pensaert M. B. Edited by Amsterdam: Elsevier Science;
    [Google Scholar]
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