1887

Abstract

Vaccination with DNA constructs encoding viral antigens has been shown to induce antiviral immunity in various model hosts. However, relevant natural virus-host systems have so far been analysed to only a very limited extent. To test the efficacy of DNA vaccination in an economically important large animal, pigs were immunized against Aujeszky’s disease, a serious virus infection caused by the alphaherpesvirus pseudorabies virus (PrV), which is characterized by severe central nervous and respiratory symptoms. After vaccination with plasmid vectors containing genes for immunogenic envelope glycoproteins C or D (gC or gD) of PrV under control of the major immediate early promotor of human cytomegalovirus, animals developed serum antibodies which recognized the respective antigen in immunoblot and exhibited neutralizing activity. Animals vaccinated with the gC expression plasmid were fully protected against a lethal challenge with PrV strain 75V19,and showed partial protection against the highly virulent NIA-3 strain. In contrast, protection was not observed after vaccination with the gD plasmid. Three intramuscular or intradermal immunizations with as little as 1 μg of gC plasmid DNA resulted in seroconversion and partial protection against lethal NIA- 3 infection. Specific antibodies were detected until at least 9 months after vaccination. In addition, a cellular immune response specific for gC could be demonstrated in proliferation assays of peripheral mononuclear lymphocytes. Our results thus demonstrate the potency of DNA vaccination for protection of large animals against a lethal virus infection.

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1997-09-01
2024-03-28
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References

  1. Andries K., Pensaert M. B., Vandeputte J. 1978; Effect of experimental infection with pseudorabies virus on pigs with maternal immunity from vaccinated sows. American Journal of Veterinary Research 39:1282–1285
    [Google Scholar]
  2. Baskerville A. 1973; The histopathology of experimental pneumonia in pigs produced by a strain of Aujeszky’s disease virus. Research in Veterinary Science 14:223–228
    [Google Scholar]
  3. Ben-Porat T., DeMarchi J. M., Lomniczi B., Kaplan A. S. 1986; Role of glycoproteins of pseudorabies virus in eliciting neutralizing antibodies. Virology 154:325–334
    [Google Scholar]
  4. Ciernik F., Berzofsky J. A., Carbone D. B. 1996; Induction of cytotoxic T lymphocytes and antitumor immunity with DNA vaccines expressing single T cell epitopes. Journal of Immunology 156:2369–2375
    [Google Scholar]
  5. Coe N. E., Mengeling W. 1990; Mapping and characterization of neutralizing epitopes of glycoproteins gIII and gp50 of the Indiana-Funkhauser strain of pseudorabies virus. Archives of Virology 110:137–142
    [Google Scholar]
  6. Cox G., Zamb T. J., Babiuk L. A. 1993; Bovine herpesvirus 1: immune response in mice and cattle injected with plasmid DNA. Journal of Virology 67:5664–5667
    [Google Scholar]
  7. Eloit M., Fargeaud D., Haridon R. L., Thoma B. 1988; Identification of pseudorabies virus glycoprotein gp50 as a major target of neutralizing antibodies. Archives of Virology 99:45–56
    [Google Scholar]
  8. Fynan E. F., Robinson H. L., Webster R. G. 1993; Use of DNA encoding influenza hemagglutinin as an avian influenza vaccine. DNA and Cell Biology 12:785–789
    [Google Scholar]
  9. Gonzales A. J. C., Morello C. S., Cranmer L. D., Spector D. H. 1996; DNA immunization confers protection against murine cyto-megalovirus infection. Journal of Virology 70:7921–7928
    [Google Scholar]
  10. Graham F. L., van der Eb A. J. 1973; A new technique for the assay of infectivity of human adenovirus. Virology 52:452–467
    [Google Scholar]
  11. Hampl H., Ben-Porat T., Ehrlicher L., Habermehl K.-O., Kaplan A. S. 1984; Characterization of the envelope proteins of pseudorabies virus. Journal of Virology 52:583–590
    [Google Scholar]
  12. Hasset D., Whitton J. L. 1996; DNA immunization. Trends in Microbiology 4:307–312
    [Google Scholar]
  13. Justewicz D. M., Morin M. J., Robinson H. L., Webster R. G. 1995; Antibody-forming cell response to virus challenge in mice immunized with DNA encoding the influenza virus hemagglutinin. Journal of Virology 69:7712–7717
    [Google Scholar]
  14. Kaplan A. S., Vatter A. 1959; A comparison of herpes simplex and pseudorabies viruses. Virology 7:394–407
    [Google Scholar]
  15. Klupp B., Visser N., Mettenleiter T. C. 1992; Identification and characterization of pseudorabies virus glycoprotein H. Journal of Virology 66:3048–3055
    [Google Scholar]
  16. Kopp A., Mettenleiter T. C. 1992; Stable rescue of a glycoprotein gII deletion mutant of pseudorabies virus by glycoprotein gI of bovine herpesvirus 1. Journal of Virology 66:2754–2762
    [Google Scholar]
  17. Kost T. A., Jones E., Smith K., Reed A. P., Brown A., Miller T. 1989; Biological evaluation of glycoproteins mapping to two distinct mRNAs within the Bam HI fragment 7 of pseudorabies virus: expression of the coding regions by vaccinia virus. Virology 171:365–376
    [Google Scholar]
  18. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  19. Lukács N., Thiel H.-J., Mettenleiter T. C., Rziha H.-J. 1985; Demonstration of three major species of pseudorabies virus glycoproteins and identification of a disulfide-linked glycoprotein complex. Journal of Virology 53:166–173
    [Google Scholar]
  20. Manickan E., Rouse R. J. D., Yu Z., Wire W. S., Rouse B. T. 1995; Genetic immunization against herpes simplex virus - protection is mediated by CD4+ T lymphocytes. Journal of Immunology 155:259–265
    [Google Scholar]
  21. Marchioli C., Yancey R., Petrovskis E., Timmins J., Post L. 1987; Evaluation of pseudorabies virus glycoprotein gp50 as a vaccine for Aujeszky’s disease in mice and swine: expression by vaccinia virus and Chinese hamster ovary cells. Journal of Virology 61:3977–3982
    [Google Scholar]
  22. Marchioli C., Yancey R. J., Timmins J. G., Post L. E., Young B. R., Povendo D. A. 1988; Protection of mice and swine from pseudorabies virus induced mortality by administration of pseudorabies specific mouse monoclonal antibodies. American Journal of Veterinary Research 49:860–864
    [Google Scholar]
  23. Martin S., Cantin E., Rouse B. T. 1988; Cytotoxic T lymphocytes - their relevance in herpesvirus infections. Annals of the New York Academy of Science 532:257–272
    [Google Scholar]
  24. Mettenleiter T. C. 1994; Pseudorabies (Aujeszky’s Disease) virus: state of the art. Acta Veterinaria Hungarica 42:153–177
    [Google Scholar]
  25. Mettenleiter T. C. 1996; Immunobiology of pseudorabies (Aujeszky’s Disease). Veterinary Immunology and Immunopathology 54:221–229
    [Google Scholar]
  26. Monteil M., Le Potier M., Guillotin J., Cariolet R., Houdayer C., Eloit M. 1996; Genetic immunization of seronegative one-day-old piglets against pseudorabies induces neutralizing antibodies but not protection and is ineffective in piglets from immune dams. Veterinary Research 27:443–452
    [Google Scholar]
  27. Mukamoto M., Watanabe I., Kobayashi Y., Icatlo F., Ishii H., Kodama Y. 1991; Immunogenicity in Aujeszky’s disease virus structural glycoprotein gpIV (gp50) in swine. Veterinary Microbiology 29:109–121
    [Google Scholar]
  28. Pedroza M. L., Lau L. L., Asano M. S., Ahmed R. 1995; DNA vaccination against persistent viral infection. Journal of Virology 69:2574–2582
    [Google Scholar]
  29. Rauh I., Mettenleiter T. C. 1991; Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration. Journal of Virology 65:5348–5356
    [Google Scholar]
  30. Raz E., Carson D. A., Parker S., Parr T. B., Abai A. M., Aichinger G., Gromkowski S. H., Singh M., Lew D., Yankauckas M. A., Baird S. M., Rhodes G. H. 1994; Intradermal gene immunization: the possible role of DNA uptake in the induction of cellular immunity to viruses. Proceedings of the National Academy of Sciences, USA 91:9519–9523
    [Google Scholar]
  31. Reddehase M. 1989; Immunity to viruses. Immunology Today 10:S39–S41
    [Google Scholar]
  32. Robbins A., Watson R., Whealy M., Hays W., Enquist L. 1986; Characterization of a pseudorabies virus glycoprotein gene with homology to herpes simplex virus type 1 and type 2 glycoprotein C. Journal of Virology 58:339–347
    [Google Scholar]
  33. Robinson H. L., Hunt L. A., Webster R. G. 1993; Protection against a lethal influenza virus challenge by immunization with a haemagglutininexpressing plasmid DNA. Vaccine 11:957–960
    [Google Scholar]
  34. Sakaguchi M., Nakamura H., Sonoda K., Hamada F., Hirai K. 1996; Protection of chickens from Newcastle disease by vaccination with a linear plasmid DNA expressing the F protein of Newcastle disease virus. Vaccine 14:747–752
    [Google Scholar]
  35. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual,, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  36. Schmidt J., Klupp B. G., Karger A., Mettenleiter T. C. 1997; Adaptability in herpesviruses : glycoprotein D-independent infectivity of pseudorabies virus. Journal of Virology 71:17–24
    [Google Scholar]
  37. Schreurs C., Mettenleiter T. C., Zuckermann F., Sugg N., Ben-Porat T. 1988; Glycoprotein gIII of pseudorabies virus is multifunctional. Journal of Virology 62:2251–2257
    [Google Scholar]
  38. Tang D., DeVit M., Johnston S. A. 1992; Genetic immunization is a simple method for eliciting an immune response. Nature 356:152–154
    [Google Scholar]
  39. Tsuda T., Onodera T., Sugimura T., Murakami Y. 1992; Induction of protective immunity and neutralizing antibodies to pseudorabies virus by immunization with anti-idiotypic antibodies. Archives of Virology 122:291–300
    [Google Scholar]
  40. Tsuchida A., Katayama S., Okada N., Okabe T., Sasaki N. 1992; Protection from pseudorabies virus challenge in mice by a combination of purified gII, gIII, and gVI antigens. Journal of Veterinary Medical Science 54:447–452
    [Google Scholar]
  41. Ulmer J. B., Donnelly J. J., Parker S. E., Rhodes G. H., Felgner P. L., Dwarki V. J., Gromkowski S. H., Deck R. R., DeWitt C. M., Friedman A., Hawe L. A., Leander K. R., Martinez D., Perry H. C., Shiver J. W., Montgomery D. L., Liu M. A. 1993; Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259:1745–1749
    [Google Scholar]
  42. Ulmer J., Sadoff J., Liu M. A. 1996; DNA vaccines. Current Opinion in Immunology 8:531–536
    [Google Scholar]
  43. Wathen M. W., Wathen L. M. K. 1984; Isolation, characterization and physical mapping of a pseudorabies virus mutant containing antigenically altered gp50. Journal of Virology 51:57–62
    [Google Scholar]
  44. Yokohama M., Zhang J., Whitton L. J. 1995; DNA immunization confers protection against lethal lymphocytic choriomeningitis virus infection. Journal of Virology 69:2684–2688
    [Google Scholar]
  45. Zuckermann F. A., Zsak L., Mettenleiter T. C., Ben-Porat T. 1990; Pseudorabies virus glycoprotein gIII is a major target antigen for murine and swine virus-specific cytotoxic T lymphocytes. Journal of Virology 64:802–812
    [Google Scholar]
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