%0 Journal Article %A Vaquero, C. %A Liao, Y. -C. %A Nähring, J. %A Fischer, R. %T Mapping of the RNA-binding domain of the cucumber mosaic virus movement protein %D 1997 %J Journal of General Virology, %V 78 %N 8 %P 2095-2099 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-78-8-2095 %I Microbiology Society, %X A series of in-frame deletion mutants was used to identify a domain within the 3a protein of cucumber mosaic virus (CMV) that is required for RNA-binding activity. Deletions in the 3a gene were generated by PCR and restriction digestion, and the resulting mutated 3a sequences were cloned either in pT7-7 or in pGEX-5X3 expression vectors. The mutated 3a proteins or fusions with glutathione S-transferase (GST) were expressed in E. coli, purified, and their nucleic acid-binding activities analysed by photochemical UV cross-linking assays using digoxi- genin-UTP-labelled RNA probes. Comparative analyses of seven mutated 3a proteins obtained from inclusion bodies and eight GST fusion proteins revealed that there is an RNA-binding domain located between amino acids 174 and 233. This RNA-binding domain is able to bind single-stranded RNA out of the context of the complete 3a movement protein and is highly conserved within both subgroups of CMV. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-8-2095