1887

Abstract

The structural phenotype of the movement proteins (MPs) of two representatives of the alfalfa mosaic virus (AMV) and brome mosaic virus (BMV), was studied in protoplasts. Immunofluorescence microscopy showed that the MPs of these viruses, for which there has been no evidence of a tubule-guided mechanism, assemble into long tubular structures at the surface of the infected protoplast. Electron microscopy and immunogold analysis confirmed the presence of both MP and virus particles in the tubules induced by AMV and BMV. The significance of the tubule-forming properties of these viral MPs is discussed.

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1997-08-01
2024-04-19
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References

  1. Dolja V. V., Haldeman R., Robertson N. L., Dougherty W. G., Carrington J. C. 1994; Distinct functions of capsid protein in assembly and movement of tobacco etch potyvirus. EMBO Journal 12:1482–1491
    [Google Scholar]
  2. Eggen R., Verver J., Wellink J., De Jong A., Goldbach R., VanKammen A. 1989; Improvements of the infectivity of in vitro transcripts from cloned cowpea mosaic virus cDNA: impact of terminal nucleotide sequences. Virology 173:447–455
    [Google Scholar]
  3. Flasinski S., Dzianott A., Pratt S., Bujarski J. 1995; Mutational analysis of the coat protein gene of brome mosaic virus: effects on replication and movement in barley and in Chenopodium hybridum . Molecular Plant-Microbe Interactions 8:23–31
    [Google Scholar]
  4. Francki R. I. B., Milne R. G., Hatta T. 1985; Cucumovirus group. In An Atlas of Plant Viruses II pp. 53–100 Boca Raton, Fla: CRC Press;
    [Google Scholar]
  5. Godefroy-Colburn T., Schoumacher F., Erny C., Berna A., Moser O., Gagey M. -J., Stussi-Garaud C. 1990; The movement of some plant viruses. In Recognition and Response in Plant-Virus Interactions NATO ASI series H41 pp. 207–321 Fraser R. S. S. Edited by Berlin & Heidelberg: Springer-Verlag;
    [Google Scholar]
  6. Johnson J. E., Argos P. 1985; Virus particle stability and structure. In The Plant Viruses. Polyhedral Virions with Tripartite Genomes pp. 19–49 Francki R. I. B. Edited by New York: Plenum Press;
    [Google Scholar]
  7. Lucas W. J., Gilbertson R. L. 1994; Plasmodesmata in relation to viral movement within leaf tissue. Annual Review of Phytopathology 32:387–411
    [Google Scholar]
  8. Martelli G. P., Russo M. 1985; Virus-host relationships : sympto- matological and ultrastructural aspects. In The Plant Viruses.Polyhedral Virions with Tripartite Genomes pp. 163–201 Francki R. I. B. Edited by New York: Plenum Press;
    [Google Scholar]
  9. Oparka K. J., Roberts A. G., Roberts I. M., Prior D. A. M., SantaCruz S. 1996; Viral coat protein is targeted to, but does not gate, plasmodesmata during cell-to-cell movement of potato virus X. Plant Journal 10:805–813
    [Google Scholar]
  10. Rao A. L. N., Grantham G. L. 1995; Biological significance of the seven amino-terminal basic residues of brome mosaic virus coat protein. Virology 211:42–52
    [Google Scholar]
  11. Tomenius K., Clapham D., Meshi T. 1987; Localization by immunogold cytochemistry of the virus-coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus. Virology 160:363–371
    [Google Scholar]
  12. Van der Vossen E. A. G., Neeleman L., Bol J. F. 1994; Early and late functions of alfalfa mosaic virus coat protein can be mutated separately. Virology 202:891–903
    [Google Scholar]
  13. Van Lent J., Wellink J., Goldbach R. 1990; Evidence for the involvement of the 58K and 48K proteins in the intracellular movement of cowpea mosaic virus. Journal of General Virology 71:219–223
    [Google Scholar]
  14. Van Lent J., Storms M., Van der Meer F., Wellink J., Goldbach R. 1991; Tubular structures involved in movement of cowpea mosaic virus are also formed in infected cowpea protoplasts. Journal of General Virology 72:2615–2623
    [Google Scholar]
  15. Van Pelt-Heerschap H., Verbeek H., Huisman M. J., Loesch-Fries L. S., Van Vloten-Doting L. 1987; Non-structural proteins and RNAs of alfalfa mosaic virus synthesized in tobacco and cowpea protoplasts. Virology 161:190–197
    [Google Scholar]
  16. Wolf S., Deom C. M., Beachy R. N., Lucas W. J. 1989; Movement protein of tobacco mosaic virus modifies plasmodesmatal size exclusion limit. Science 246:377–379
    [Google Scholar]
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