1887

Abstract

Monoclonal antibodies (MAbs) raised against the coat protein of zucchini yellow mosaic potyvirus (ZYMV) were characterized by epitope mapping using synthetic oligopeptides. Two mutant viruses with a mutation in the amino acid sequence important for epitope recognition were obtained by site-directed mutagenesis of a full- length cDNA of ZYMV. Two MAbs, CC11 and DD2, could distinguish specifically between these mutants in mixed infections, or after sequential inoculations of muskmelons. Sequential inoculations of the mutants and analysis with MAbs CC11 and DD2 revealed that cross-protection was established between these quasi-isogenic strains within 48 h.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-8-2073
1997-08-01
2022-05-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/8/9267009.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-8-2073&mimeType=html&fmt=ahah

References

  1. Andreeva L., Järvekülg L., Rabenstein F., Torrance L., Harrison B. D., Saarma M. 1994; Antigenic analysis of potato virus A particles and coat protein. Annals of Applied Biology 125:337–348
    [Google Scholar]
  2. Atreya C. D., Raccah B., Pirone T. P. 1990; A point mutation in the coat protein abolishes aphid transmissibility of a potyvirus. Virology 178:161–165
    [Google Scholar]
  3. Barbara D. J., Morton A., Spence N. J., Miller A. 1995; Rapid differentiation of closely related isolates of two plant viruses by polymerase chain reaction and restriction fragment length polymorphism analysis. Journal of Virological Methods 55:121–131
    [Google Scholar]
  4. Desbiez C., Wipf-Scheibel C., Granier F., Robaglia C., Delaunay T., Lecoq H. 1996; Biological and molecular variability of zucchini yellow mosaic virus in the island of Martinique. Plant Disease 80:203–207
    [Google Scholar]
  5. Domingo E., Holland J. J. 1994; Mutation rates and rapid evolution of RNA viruses. In The Evolutionary Biology of Viruses pp. 161–184 Morse S. S. Edited by New York: Raven Press;
    [Google Scholar]
  6. Gal-On A., Antignus Y., Rosner A., Raccah B. 1991; Infectious in vitro RNA transcripts derived from cloned cDNA of the cucurbit potyvirus, zucchini yellow mosaic virus. Journal of General Virology 72:2639–2643
    [Google Scholar]
  7. Gal-On A., Antignus Y., Rosner A., Raccah B. 1992; A zucchini yellow mosaic virus coat protein gene mutation restores aphid transmissibility but has no effect on multiplication. Journal of General Virology 73:2183–2187
    [Google Scholar]
  8. Gal-On A., Meiri E., Huet H., Hua W. J., Raccah B., Gaba V. 1995; Particle bombardment drastically increases the infectivity of cloned DNA of zucchini yellow mosaic potyvirus. Journal of General Virology 76:3223–3227
    [Google Scholar]
  9. Geysen H. M., Meloen R. H., Barteling S. J. 1984; Use of peptide synthesis to probe viral antigen for epitopes to a resolution of single amino acid. Proceedings of the National Academy of Sciences, USA 81:3998–4002
    [Google Scholar]
  10. Kunkel T. A. 1985; Rapid and efficient site-specific mutagenesis without phenotypic selection. Proceedings of the National Academy of Sciences, USA 82:488–492
    [Google Scholar]
  11. Lecoq H., Pitrat M. 1984; Strains of ZYMV in muskmelon (Cucumis melo L.). Phytopathologische Zeitschrift 111:165–173
    [Google Scholar]
  12. Lecoq H., Wipf-Scheibel C., Lemaire J. M. 1991; Control of ZYMV in squash by cross protection. Plant Disease 75:208–211
    [Google Scholar]
  13. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5469
    [Google Scholar]
  14. Shukla D. D., Strike P. M., Tracy S. L., Gough K. H., Ward C. W. 1988; The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. Journal of General Virology 69:1497–1508
    [Google Scholar]
  15. Shukla D. D., Tribbick G., Mason T. J., Hewish D. R., Geysen H. M., Ward C. W. 1989; Localization of virus-specific and group-specific epitopes of plant potyviruses by systematic immunochemical analysis of overlapping peptide fragments. Proceedings of the National Academy of Sciences, USA 86:8192–8196
    [Google Scholar]
  16. Walkey D. G. A., Lecoq H., Collier R., Dobson S. 1992; Studies on the control of zucchini yellow mosaic virus in courgettes by mild strain protection. Plant Pathology 41:762–771
    [Google Scholar]
  17. Wang H. L., Gonsalves D., Provvidenti R., Lecoq H. 1991; Effectiveness of cross protection by a mild strain of zucchini yellow mosaic virus in cucumber, melon and squash. Plant Disease 75:203–207
    [Google Scholar]
  18. Ward C. W., McKern N. M., Frenkel M. J., Shukla D. D. 1992; Sequence data as the major criterion for potyvirus classification. Archives of Virology Supplementum 5:283–297
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-8-2073
Loading
/content/journal/jgv/10.1099/0022-1317-78-8-2073
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error