@article{mbs:/content/journal/jgv/10.1099/0022-1317-78-8-2067, author = "Jelkmann, Wilhelm and Fechtner, Beate and Agranovsky, Alexey A.", title = "Complete genome structure and phylogenetic analysis of little cherry virus, a mealybug-transmissible closterovirus", journal= "Journal of General Virology", year = "1997", volume = "78", number = "8", pages = "2067-2071", doi = "https://doi.org/10.1099/0022-1317-78-8-2067", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-8-2067", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The 5 ′ -terminal genomic region (8597 nt) of little cherry virus (LChV), a mealybug-borne closterovirus, was cloned from double-stranded RNA, and its sequence determined to complete the 16934 nt sequence of the monopartite LChV RNA genome. In the 5′ to 3′ direction, the sequence encompasses ORF 1a, encoding the conserved replicative domains of methyltransferase and helicase, and ORF 1b, encoding RNA polymerase. ORFs 1a and 1b partially overlap (in 0/ 1 configuration), and the LChV replicase is probably expressed by ribo- somal frameshifting as a fusion product with a molecular mass of 318 kDa. The N-terminal part of the ORF 1a product contains a papain-like cysteine proteinase (PCP) domain with a predicted cleavage site between Gly-619 and Ser-620. The PCP and the upstream protein domains can be aligned with the equivalent parts of the leader proteins encoded by the whitefly-transmitted lettuce infectious yellows and sweet potato sunken vein clostero- viruses. Phylogenetic reconstruction based on the aligned RNA polymerase sequences clearly suggests that the aphid-transmissible and whitefly- transmissible closteroviruses represent two distinct evolutionary lineages, with the mealybug-transmissible LChV being the most remote member of the ‘whitefly’ lineage.", }