RT Journal Article SR Electronic(1) A1 Ito, Masahiro A1 Koide, Waka A1 Watanabe, Masahiro A1 Kamiya, Hitoshi A1 Sakurai, MinoruYR 1997 T1 Apoptosis of cord blood T lymphocytes by herpes simplex virus type 1 JF Journal of General Virology, VO 78 IS 8 SP 1971 OP 1975 DO https://doi.org/10.1099/0022-1317-78-8-1971 PB Microbiology Society, SN 1465-2099, AB We investigated apoptosis induced by herpes simplex virus type 1 (HSV-1) in cord blood T lymphocytes by using agarose gel electrophoresis, DNA content analysis and the terminal deoxytransferase (TdT)-mediated dUTP nick end-labelling (TUNEL) method. DNA fragmentation and the hypodiploid fraction in the cell cycle were both increased in HSV-1-infected CD4 and CD8 lymphocytes stimulated with phytohaemagglutinin (PHA) compared to mock-infected lymphocytes. The percentage of cells in the S phase was decreased in HSV-1-infected CD4 and CD8 lymphocytes. HSV-1 antigen, glycoprotein D (gD) and regulatory protein ICP27 were detected in 8–18% of the hypodiploid fraction of PHA-stimulated, HSV-1-infected lymphocytes. Apoptosis was induced not only in HSV-1 antigenexpressing cells but also in cells not expressing detectable viral proteins. Addition of anti-Fas antibody, anti-Fas-ligand antibody or a mixture of both had no effect on HSV-1-induced apoptosis, indicating that the Fas-Fas-ligand pathway did not contribute to HSV-1-induced apoptosis., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-8-1971