1887

Abstract

BALB/c mice were immunized with the EP3 surface epitope of the mouse mammary tumour virus (MMTV) gp52 envelope protein before systemic infection with MMTV(C3H) or MMTV(SW). Analysis of the successive stages of the virus infection showed that although these mice were protected against mammary tumour formation, earlier stages of the infection were not inhibited, as reflected by the persisting superantigen-induced activation and deletion of V-specificT cells. Transplacental transfer of maternal anti-EP3 immunoglobulins to newborns did not protect them from infection through the Peyer’s patches. Preincubation of the MMTVs with an anti-EP3 serum before injection, however, successfully inhibited the early stages of the infection. Results from this study show that to inhibit infection by MMTV efficiently, the virus must be neutralized before its interaction with the cell membrane, and that the affinity of the virus- membrane interaction is higher than that of the virus-antibody interaction.

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/content/journal/jgv/10.1099/0022-1317-78-8-1935
1997-08-01
2022-05-26
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References

  1. Acha Orbea H., Shakhov A. N., Scarpellino L., Kolb E., Müller V., Vessaz-Shaw A., Fuchs R., Blochlinger K., Rollini P., Billotte J., Sarafidou M., MacDonald H. R., Diggelmann H. 1991; Clonal deletion of V beta 14 positive T cells in mammary tumor virus transgenic mice. Nature 350:207–211
    [Google Scholar]
  2. Allan C. H., Trier J. S. 1991; Structure and permeability differ in subepithelial villus and Peyer’s patch follicle capillaries. Gastroenterology 100:1172–1179
    [Google Scholar]
  3. Astori M., Kraehenbuhl J. P. 1996; Recombinant fusion peptides containing multiple repeats of a ubiquitous T helper epitope are highly immunogenic. Molecular Immunology 33:1017–1024
    [Google Scholar]
  4. Bittner J. J. 1942; The milk influence of breast tumors in mice. Science 95:462–463
    [Google Scholar]
  5. Choi Y., Kappler W., Marrack P. 1991; A superantigen encoded in the open reading frame of the 3′ long terminal repeat of mouse mammary tumor virus. Nature 350:203–207
    [Google Scholar]
  6. Choi Y., Marrack P., Kappler J. W. 1992; Structural analysis of a mouse mammary tumor virus superantigen. Journal of Experimental Medicine 175:847–852
    [Google Scholar]
  7. Charney J., Holben J. A., Cody C. M., Moore D. H. 1976; Further immunization studies with mammary tumor virus. Cancer Research 36:777–780
    [Google Scholar]
  8. Dion A. S., Knittel J. J., Morneweck S. T. 1990; Virus envelope- based peptide vaccines against virus-induced mammary tumors. Virology 179:474–477
    [Google Scholar]
  9. Girardi A. J., Dion A. S., Pomenti A. A., Holben J. A. 1985; Comparative studies of purified subviral component vaccines and formalin-treated mammary tumor viruses from four inbred strains of mice. Journal of the National Cancer Institute 74:405–413
    [Google Scholar]
  10. Hageman Ph. C., Calafat J., Hilgers J. 1981; The biology of the mouse mammary tumor virus. In Mammary Tumors in the Mouse p 391 Hilgers J., Sluyser M. Edited by Amsterdam: Elsevier/North Holland Biomedical Press;
    [Google Scholar]
  11. Held W., Shakhov A. N., Waanders G., Scarpellino L., Kraehenbuhl J. P., MacDonald H. R., AchaOrbea H. 1992; An exogenous mouse mammary tumor virus with properties of mls-1a (mtv-7). Journal of Experimental Medicine 175:1623–1633
    [Google Scholar]
  12. Karapetian O., Shakhov A. N., Kraehenbuhl J. P., Acha Orbea H. 1994; Retroviral infection of neonatal Peyer’s patch lymphocytes : the mouse mammary tumor virus model. Journal of Experimental Medicine 180: 1511–1516
    [Google Scholar]
  13. Marrack P., Kushnir E., Kappler J. W. 1991; A maternally inherited superantigen encoded by a mammary tumour virus. Nature 349:524
    [Google Scholar]
  14. Nusse R., Van Ooyen A., Cox D., Fung Y. K., Varmus H. 1984; Mode of proviral activation of a putative mammary oncogene (int-1) on chromosome 15. Nature 307:131–136
    [Google Scholar]
  15. Sarkar N., Moore D. 1978; Immunization of mice against murine mammary tumor virus infection and mammary tumor development. Cancer Research 38:1468–1472
    [Google Scholar]
  16. St George J. A., Cardiff R. D., Young L. J., Faulkin L. J. 1978; The distribution of mouse mammary tumor virus (MMTV) antigens in BALB/cfC3H mammary tissues. Proceedings of the American Association for Cancer Research 19:51
    [Google Scholar]
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