1887

Abstract

Infectious salmon anaemia virus (ISAV), which previously had never been isolated in any of the commercially available established fish cell lines, was successfully propagated in the continuous cell line Atlantic salmon (AS). The yield of infectious ISAV increased with the incubation time of virus- inoculated cells, demonstrated by infectivity trials in groups of Atlantic salmon. Trypsin treatment of the virus was not necessary for primary infection of AS cells with salmon-grown ISAV. The infection was non-cytopathic, but it was possible to detect virus-infected cells by a haemadsorption centre assay using Atlantic salmon erythrocytes. Pleomorphic enveloped virus particles were seen by transmission electron microscopy of infected AS cells. Elongated forms were observed, but spherical particles with diameters of 90–130 nm were commonest. Growth of ISAV was inhibited by actino- mycin D but not by 5-bromo-2-deoxyuridine treatment, which indicates that ISAV may be an aquatic orthomyxovirus.

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1997-08-01
2022-08-15
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References

  1. Barry R. D., Ives D. R., Cruickshank J. G. 1962; Participation of deoxyribonucleic acid in the multiplication of influenza virus. Nature 194:1139–1140
    [Google Scholar]
  2. Boycott R., Klenk H. -D., Ohuchi M. 1994; Cell tropism of influenza virus mediated by hemagglutinin activation at the stage of virus entry. Virology 203:313–319
    [Google Scholar]
  3. Dannevig B. H., Falk K., Namork E. 1995; Isolation of the causal virus of infectious salmon anaemia (ISA) in a long-term cell line from Atlantic salmon head kidney. Journal of General Virology 76: 1353–1359
    [Google Scholar]
  4. Dulbecco R. 1980; The nature of viruses. In Microbiology, 3rd edn. pp 854–884 Davis B. D., Dulbecco R., Eisen H. N., Ginsberg H. S. Edited by Philadelphia: Harper & Row;
    [Google Scholar]
  5. Klenk H. -D., Rott R. 1988; The molecular biology of influenza virus pathogenicity. Advances in Virus Research 34:247–281
    [Google Scholar]
  6. Klenk H. -D., Rott R., Orlich M., Blodorn J. 1975; Activation of influenza A viruses by trypsin treatment. Virology 68: 426–439
    [Google Scholar]
  7. Murphy B. R., Webster R. G. 1990; Orthomyxoviruses. In Virology,, 2nd edn. pp 1091–1152 Fields B. N., Knipe D. M. Edited by New York: Raven Press;
    [Google Scholar]
  8. Nagabayashi T., Wolf K. 1979; Characterization of EV-2, a virus isolated from European eels (Anguilla anguilla) with stomatopapilloma. Journal of Virology 30:358–364
    [Google Scholar]
  9. Neukirch M. 1985; Isolation of an orthomyxovirus-like agent from eel (Anguilla anguilla). Bulletin ofthe European Association of Fish Pathologists 5:12–13
    [Google Scholar]
  10. Norrby E., Marusyk H., Ürvell C. 1970; Morphogenesis of respiratory syncytial virus in green monkey kidney cell line (Vero). Journal of Virology 6: 237–242
    [Google Scholar]
  11. Nylund A., Alexandersen S., Løvik P., Jakobsen P. 1994; The response of brown trout (Salmo trutta L.) to repeated challenge with infectious salmon anaemia (ISA). Bulletin ofthe European Association of Fish Pathologists 14:167–170
    [Google Scholar]
  12. Nylund A., Hovland T., Watanabe K., Endresen C. 1995; Presence of infectious salmon anaemia virus (ISAV) in tissues of Atlantic salmon, Salmo salar L., collected during three separate outbreaks of the disease. Journal of Fish Diseases 18:135–145
    [Google Scholar]
  13. Sommer A. -I., Mennen S. 1996; Propagation of infectious salmon anaemia virus in Atlantic salmon, Salmo salar L., head kidney macrophages. Journal of Fish Diseases 19:179–183
    [Google Scholar]
  14. Stephenson J. R., Dimmock N. J. 1975; Early events in influenza virus multiplication. I. Location and fate of the input RNA. Virology 65:77–86
    [Google Scholar]
  15. Thorud K., Djupvik H. O. 1988; Infectious anaemia in Atlantic salmon (Salmo salar L.). Bulletin of the European Association of Fish Pathologists 8:109–111
    [Google Scholar]
  16. Tobita K., Sugiura A., Enomoto C., Furyama M. 1975; Plaque assay and primary isolation of influenza A viruses in an established line of canine kidney cells (MDCK) in the presence of trypsin. Medical Microbiology and Immunology 162:9–14
    [Google Scholar]
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