1887

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Volume 78, Issue 8

Influence of the 5′ noncoding region of hepatitis A virus strain GBM on its growth in different cell lines Free

Abstract

Previous sequence analysis of consecutive passages of the hepatitis A virus (HAV) strain GBM/WT in human embryonic kidney cells (HEK cells), human embryonic lung fibroblasts (HFS cells) and in FRhK- 4 cells (foetal rhesus monkey kidney cells) pointed to a host cell dependent cell culture adaptation of GBM/WT in HFS cells involving mutations in the 5′ noncoding region (5′ NCR). Multiple nucleotide changes occurred in the 5′ NCR of the GBM genome after the cell line used for virus passage was changed from HEK cells to HFS cells. In contrast, no mutations in the 5′ NCR occurred during the first 20 passages of GBM/WT in FRhK-4 cells. In order to analyse the influence of the 5′ NCR on host cell specific adaptation of HAV strain GBM in different cell cultures, GBM/HM175 chimeras were constructed which contained 5 NCRs from different GBM variants by replacing the 5′ NCR of the infectious clone pHAV/7. Parallel transfection assays in FRhK-4 and HFS cells, performed with transcripts from the chimeric GBM/HM175 constructs, showed that the 5′ NCR of the GBM variant GBM/HFS is essential for virus growth in HFS cells. The GBM/HM175 chimeric RNA, which contained the 5′ NCR of GBM/HFS, exclusively, was able to produce infectious virus after transfection of HFS cells. The growth of the different GBM/HM175 chimeras in FRhK-4 cells, in contrast, did not seem to be strongly influenced by a specific sequence of the 5′ NCR.

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© Society for General Microbiology Ltd 1997
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1997-08-01
2024-04-18
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References

  1. Anderson D. A. 1987; Cytopathology, plaque assay and heat inactivation of hepatitis A virus strain HM175. Journal of Medical Virology 22:35–44
     [Google Scholar]
  2. Andino R., Rieckhof G. E., Achacoso P. L., Baltimore D. 1993; Poliovirus RNA synthesis utilizes an RPN complex formed around the 5′- end of viral RNAEMBO Journal. 12:3587–3598
     [Google Scholar]
  3. Borman A. M., Deliat F. G., Kean K. M. 1994; Sequences within the poliovirus internal ribosome entry segment control viral RNA synthesis. EMBO Journal 13:3149–3157
     [Google Scholar]
  4. Brown E. A., Day S. P., Jansen R. W., Lemon S. M. 1991; The 5′ nontranslated region of hepatitis A virus RNA: secondary structure and elements required for translation in vitro. Journal of Virology 65:5828–5838
     [Google Scholar]
  5. Brown E. A., Zalac A. J., Lemon S. M. 1994; In vitro characterization of an internal ribosome entry site (IRES) present within the 5′ nontranslated region of hepatitis A virus RNA: comparison with the IRES of encephalomyocarditis virus. Journal of Virology 68:1066–1074
     [Google Scholar]
  6. Carneiro J. S., Equestre M., Pagnotti P., Gradi A., Sonenberg N., PerezBercoff R. 1995; 5′A: mutations in the 5′-most pyrimidine-rich tract reduce its ability to direct internal initiation of translation. Journal of General Virology 76:1189–1196
     [Google Scholar]
  7. Chang K. H., Brown E. A., Lemon S. M. 1993; Cell type-specific proteins which interact with the 5′ nontranslated region of hepatitis A virus RNA. Journal of Virology 67:6716–6725
     [Google Scholar]
  8. Cohen J. I., Ticehurst J. R., Purcell R. H., Buckler-White A., Baroudy B. M. 1987a; Complete nucleotide sequence of wild-type hepatitis A virus: comparison of different strains of hepatitis A virus and other picornaviruses. Journal of Virology 61:50–59
     [Google Scholar]
  9. Cohen J. I., Rosenblum B., Ticehurst J. R., Daemer R. J., Feinstone S. M., Purcell R. H. 1987b; Complete nucleotide sequence of an attenuated hepatitis A virus: comparison with wild-type virus. Proceedings of the National Academy of Sciences USA: 842497–2501
     [Google Scholar]
  10. Cohen J. I., Ticehurst J. R., Feinstone S. M., Rosenblum B., Purcell R. H. 1987c; Hepatitis A virus cDNA and its RNA transcripts are infectious in cell culture. Journal of Virology 61:3035–3039
     [Google Scholar]
  11. Cromeans T., Fields H. A., Sobsey M. D. 1989; Replication kinetics and cytopathic effects of hepatitis A virus. Journal of General Virology 70:2051–2062
     [Google Scholar]
  12. Daemer R. J., Feinstone S. M., Gust I. D., Purcell R. H. 1981; Propagation of human hepatitis a virus in African green monkey kidney cell culture : primary isolation and serial passage. Infection and Immunity 32:388–393
     [Google Scholar]
  13. Day S. P., Lemon S. M. 1990; A single base mutation in the 5′noncoding region of HAV enhances replication of virus in vitro. In Vaccines 90 : Modern Approaches to New Vaccines including Prevention of AIDS pp 175–178 Brown F., Chanock R. M., Ginsberg H. S., Lerner R. A. Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  14. Day S. P., Murphy P., Brown E. A., Lemon S. M. 1992; Mutations within the 5′ nontranslated region of hepatitis A virus RNA which enhance replication in BS-C-1 cells. Journal of Virology 66:6533–6540
     [Google Scholar]
  15. Dotzauer A., Feinstone S. M., Kaplan G. 1994; Susceptibility of nonprimate cell lines to hepatitis A virus infection. Journal of Virology 68:6064–6068
     [Google Scholar]
  16. Emerson S. U., McRill C., Rosenblum B., Feinstone S. M., Purcell R. H. 1991; Mutations responsible for adaptation of hepatitis A virus to efficient growth in cell culture. Journal of Virology 65:4882–4886
     [Google Scholar]
  17. Emerson S. U., Huang Y. K., McRill C., Lewis M., Purcell R. H. 1992; Mutations in both the 2B and 2C genes of hepatitis A virus are involved in adaptation to growth in cell culture. Journal of Virology 66:650–654
     [Google Scholar]
  18. Flehmig B. 1980; Hepatitis A virus in cell culture. I. Propagation of different hepatitis A virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4). Journal of Medical Microbiology and Immunology 168:239–248
     [Google Scholar]
  19. Francki R. I. B., Fauquet C. M., Knudson D. L., Brown F. 1991; Classification and nomenclature of viruses. Fifth Report of the International Committee on Taxonomy of Viruses. Archives of Virology Supplementum 2:320–326
     [Google Scholar]
  20. Funkhouser A. W., Purcell R. H., D’Hondt E., Emerson S. U. 1994; Attenuated hepatitis A virus: genetic determinants of adaptation to growth in MRC-5 cells. Journal of Virology 68:148–157
     [Google Scholar]
  21. Glass M. J., Summers D. S. 1992; A cis-acting element within the hepatitis A virus 5′-non-coding region required for in vitro translation. Virus Research 26:15–31
     [Google Scholar]
  22. Glass M. J., Jia X. -J., Summers D. S. 1993; Identification of the hepatitis A virus internal ribosome entry site : In vivo and in vitro analysis of bicistronic RNAs containing the HAV 5′ noncoding region. Virology 193:842–852
     [Google Scholar]
  23. Graff J., Ticehurst J., Flehmig B. 1993; Detection of hepatitis A virus in sewage sludge by antigen-capture polymerase chain reaction. Applied and Environmental Microbiology 59:3165–3170
     [Google Scholar]
  24. Graff J., Normann A., Feinstone S. M., Flehmig B. 1994a; Nucleotide sequence of wild-type hepatitis A virus GBM in comparison to two cell culture-adapted variants. Journal of Virology 68:548–54
     [Google Scholar]
  25. Graff J., Kasang C., Normann A., Pfisterer-Hunt M., Feinstone S. M., Flehmig B. 1994b; Mutational events in consecutive passages of hepatitis A virus strain GBM during cell culture adaptation. Virology 204:60–68
     [Google Scholar]
  26. Jansen R. W., Newbold J. E., Lemon S. M. 1988; Complete nucleotide sequence of a cell culture-adapted variant of hepatitis A virus : comparison with wild-type virus with restricted capacity for in vitro replication. Virology 63:299–307
     [Google Scholar]
  27. Lemon S. M., Murphy P. C., Shields P. A., Ping L. -H., Feinstone S. M., Cromeans T., Jansen R. W. 1991; Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination. Journal of Virology 65:2056–2065
     [Google Scholar]
  28. Morace G., Pisani G., Beneduce F., Divizia M., Panà A. 1993; Mutations in the 3A genomic region of two cytopathic strains of hepatitis A virus isolated in Italy. Virus Research 28:187–194
     [Google Scholar]
  29. Nasser A. M., Metcalf T. G. 1987; Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus. Applied and Environmental Microbiology 53:2967–2971
     [Google Scholar]
  30. Paul A. V., Tada H., von der Helm K., Wissel T., Kiehn R., Wimmer E., Deinhardt F. 1987; The entire nucleotide sequence of the genome of human hepatitis A virus (isolate MBB). Virus Research 8:153–171
     [Google Scholar]
  31. Ross B. C., Anderson B. N., Edwards P. C., Gust I. D. 1989; Nucleotide sequence of high-passaged hepatitis A virus strain HM175: comparison with wild-type and cell culture-adapted strains. Journal of General Virology 70:2805–2810
     [Google Scholar]
  32. Rueckert R. R. 1990; Picornaviruses and their replication. In Virology, 2nd edn. pp507–548 Fields B. N., Knipe D. N., Chanock R. M., Melnick J. L., Roizman B., Hirsch M. S., Monath J. P. Edited by New York: Raven;
     [Google Scholar]
  33. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  34. Schultz D. E., Honda M., Whetter L. E., McKnight K. L., Lemon S. M. 1996; Mutations within the 5′ nontranslated RNA of cell culture- adapted hepatitis A virus which enhance cap-independent translation in cultured African green monkey cells. Journal of Virology 70:1041–1049
     [Google Scholar]
  35. Shaffer D. R., Brown E. A., Lemon S. M. 1994; Large deletion mutations involving the first pyrimidine-rich tract of the 5′ nontranslated RNA of human hepatitis A virus define two adjacent domains associated with distinct replication phenotypes. Journal of Virology 68:568–578
     [Google Scholar]
  36. Shaffer D. R., Lemon S. M. 1995; Temperature-sensitive hepatitis A virus mutants with deletions downstream of the first pyrimidine-rich tract of the 5′ nontranslated RNA are impaired in RNA synthesis. Journal of Virology 69: 6498–6506
     [Google Scholar]
  37. Siegl G., Lemon S. M. 1990; Recent advanced in hepatitis A vaccine development. Virus Research 17:75–92
     [Google Scholar]
  38. Tedeschi V., Purcell R. H., Emerson S. U. 1993; Partial characterization of hepatitis A virus from three intermediate passage levels of a series resulting in adaptation to growth in cell culture and attenuation of virulence. Journal of Medical Virology 39:16–22
     [Google Scholar]
  39. Venuti A., Di Russo C., Del Grosso N., Patti A. -M., Ruggeri F., DeStasio P. R., Martinello M. G., Pagnotti P., Degener A. M., Midulla M., Panè A., Perez-Bercoff R. 1985; Isolation and molecular cloning of a fast-growing strain of human hepatitis A virus from its doublestranded replication form. Journal of Virology 56:579–588
     [Google Scholar]
  40. Weitz M., Baroudy B. M., Maloy W. L., Ticehurst J. R., Purcell R. H. 1986; Detection of a genome-linked protein (VPg) of hepatitis A virus and its comparison with other picornaviral VPgs. Journal of Virology 60:124–130
     [Google Scholar]
  41. Whetter L. E., Day S. P., Elroy-Stein O., Brown E. A., Lemon S. M. 1994; Low efficiency of the 5′ nontranslated region of hepatitis A virus RNA in directing cap-independent translation in permissive monkey kidney cells. Journal of Virology 68:5253–5263
     [Google Scholar]
  42. Zhang H., Chao S. -F., Ping L. -H., Grace K., Clarke B., Lemon S. M. 1995; An infectious cDNA clone of a cytopathic hepatitis A virus : genomic regions associated with rapid replication and cytopathic effect. Virology 212:686–697
     [Google Scholar]
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Influence of the 5′ noncoding region of hepatitis A virus strain GBM on its growth in different cell lines
J. Gen. Virol. 78, 1841 (1997); https://doi.org/10.1099/0022-1317-78-8-1841
/content/journal/jgv/10.1099/0022-1317-78-8-1841
/content/journal/jgv/10.1099/0022-1317-78-8-1841
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