1887

Abstract

The major open reading frame of banana bunchy top virus (BBTV) DNA-1 encodes a putative replication initiation protein (Rep). , a fusion protein of BBTV Rep linked to a maltose-binding protein exhibited both site-specific nicking and joining activities. These activities were dependent on the presence of Mg or Mn , but did not require ATP. The fusion protein specifically cleaved ssDNA between bases 7 and 8 of a conserved nonanucleotide loop sequence which is present in the virion-strand of the stem-loop common region of each BBTV component. During this reaction, the fusion protein became covalently attached to the 5′ end of the cleavage product. After the nicking reactions, the fusion protein was also capable of catalysing the joining of two nicked ssDNA fragments in a site-specific manner. Based on these activities, BBTV Rep would appear to be very similar to the Rep proteins of the geminiviruses.

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1997-07-01
2022-05-23
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References

  1. Baas P. D., Jansz H. S. 1988; Single -stranded DNA phage origins. Current Topics in Microbiology and Immunology 136:31–70
    [Google Scholar]
  2. Boevink P., Chu P. W. G., Keese P. 1995; Sequence of subterranean clover stunt virus DNA: affinities with the geminiviruses. Virology 207:354–361
    [Google Scholar]
  3. Burns T. M., Harding R. M., Dale J. L. 1995; The genome organization of banana bunchy top virus: analysis of six ssDNA components. Journal of General Virology 76:1471–1482
    [Google Scholar]
  4. Gros M. F., te Riele H., Ehrlich S. D. 1987; Rolling circle replication of single-stranded DNA plasmid pC194. EMBO Journal 6:3863–3869
    [Google Scholar]
  5. Gruss A., Ehrlich S. D. 1989; The family of highly interrelated single-stranded deoxyribonucleic acid plasmids. Microbiology Reviews 53:231–241
    [Google Scholar]
  6. Harding R. M., Burns T. M., Hafner G. J., Dietzgen R., Dale J. L. 1993; Nucleotide sequence of one segment of the banana bunchy top genome contains a putative replicase gene. Journal of General Virology 74:323–328
    [Google Scholar]
  7. Heyraud F., Matzeit V., Kammann M., Schaefer S., Schell J., Gronenborn B. 1993; Identification of the initiation sequence for viral strand DNA synthesis of wheat dwarf virus. EMBO Journal 12:4445–4452
    [Google Scholar]
  8. Ilyina T. V., Koonin E. V. 1992; Conserved sequence motifs in the initiator proteins for rolling circle DNA replication encoded by diverse replicons from eubacteria, eucaryotes and archaebacteria. Nucleic Acids Research 20:3279–3285
    [Google Scholar]
  9. Katul L., Maiss E., Vetten H. J. 1995; Sequence analysis of a faba bean necrotic yellows virus DNA component containing a putative replicase gene. Journal of General Virology 76:475–479
    [Google Scholar]
  10. Koepsel R. R., Murray R. W., Rosenblum W. D., Khan S. A. 1985; The replication initiator protein of plasmid pT181 has sequence-specific endonuclease and topoisomerase activities. Proceedings of the National Academy of Sciences, USA 82:6045–6049
    [Google Scholar]
  11. Koonin E. V., Ilyina T. V. 1992; Geminivirus replication proteins are related to prokaryotic plasmid rolling circle DNA replication initiator proteins. Journal of General Virology 73:2763–2766
    [Google Scholar]
  12. Laufs J., Jupin I., David C., Schumacher S., Heyraud-Nitschke F., Gronenborn B. 1995a; Geminivirus replication: genetic and biochemical characterization of Rep protein function, a review. Biochimie 77:765–773
    [Google Scholar]
  13. Laufs J., Schumacher S., Geisler N., Jupin I., Gronenborn B. 1995b; Identification of the nicking tyrosine of geminivirus Rep protein. FEBS Letters 18:258–262
    [Google Scholar]
  14. Laufs J., Traut W., Heyraud F., Matzeit V., Rogers S. G., Schell J., Gronenborn B. 1995c; In vitro cleavage and joining at the viral origin of replication by the replication initiator protein of tomato yellow leaf curl virus. Proceedings of the National Academy of Sciences, USA 92:3879–3883
    [Google Scholar]
  15. Meyer T. F., Geider K. 1979; Bacteriophage fd gene II-protein. Specific cleavage and relaxation of supercoiled RF from filamentous phages. Journal of Biological Chemistry 254:12642–12646
    [Google Scholar]
  16. Moscoso M., Del Solar G., Espinosa M. 1995; Specific nicking- closing activity of the initiator of replication protein RepB plasmid pMV158 on supercoiled or single-stranded DNA. Journal of Biological Chemistry 270:3772–3779
    [Google Scholar]
  17. Pansegrau W., Ziegelin G., Lanka E. 1990; Covalent association of the tral gene product of the plasmid RP4 with the 5′-terminal nucleotide at the relaxation nick site. Journal of Biological Chemistry 265:10637–10644
    [Google Scholar]
  18. Rasooly A., Novick R. P. 1993; Replication-specific inactivation of the pT181 plasmid initiator protein. Science 262:1048–1050
    [Google Scholar]
  19. Rohde W., Randles J. W., Langridge P., Hanold D. 1990; Nucleotide sequence of a circular single-stranded DNA associated with coconut foliar decay virus. Virology 176:648–651
    [Google Scholar]
  20. Sano Y., Isogai M., Satoh S., Kojima M. 1993; Small virus-like particles containing single-stranded DNAs associated with milk vetch dwarf disease in Japan. 6th International Congress of Plant Pathology Montreal, Canada: 1993 Abstract 17.1.27 pp. 305
    [Google Scholar]
  21. Scherzinger E., Kruft V., Otto S. 1993; Purification of the large mobilization protein of plasmid RSF1010 and characterisation of its site- specific DNA-cleaving/DNA-joining activity. European Journal of Biochemistry 217:929–938
    [Google Scholar]
  22. te Riele H., Michel B., Ehrlich S. D. 1986; Single -stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus. Proceedings of the National Academy of Sciences, USA 83:2541–2545
    [Google Scholar]
  23. Thomas C. D., Balson D. F., Shaw W. V. 1990; In vitro studies of the initiation of staphylococcal plasmid replication. Specificity of RepD protein for its origin (oriD), and characterisation of the Rep-ori tyrosyl ester intermediate. Journal of Biological Chemistry 265:5519–5530
    [Google Scholar]
  24. Yasukawa H., Hase T., Sakai A., Masamune Y. 1991; Rolling- circle replication of plasmid pKYM isolated from a Gram-negative bacterium. Proceedings of the National Academy of Sciences, USA 88:10282–10286
    [Google Scholar]
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