@article{mbs:/content/journal/jgv/10.1099/0022-1317-78-7-1765, author = "Yang, Guang and Mawassi, Munir and Ashoulin, Lilach and Gafny, Ron and Gaba, Victor and Gal-On, Amit and Bar-Joseph, Moshe", title = "A cDNA clone from a defective RNA of citrus tristeza virus is infective in the presence of the helper virus.", journal= "Journal of General Virology", year = "1997", volume = "78", number = "7", pages = "1765-1769", doi = "https://doi.org/10.1099/0022-1317-78-7-1765", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-7-1765", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "A naturally occurring defective RNA of 2379 nt (D2.3) from the VT strain of citrus tristeza clostero- virus (CTV) was cloned and sequenced. The D2.3 RNA is a fusion of two regions of 1521 and 858 nt from the 5′ and 3′ ends of the CTV genome, respectively. A cDNA clone of D2.3 RNA was tagged by the insertion of a 0·47 kb chimeric DNA fragment and the recombinant cDNA was inserted downstream of the cauliflower mosaic virus 35S promoter. The resulting construct was bombarded into CTV-infected tissue, which was then grafted onto virus-free plants. The presence of recombinant RNA in systemically infected leaves was demonstrated by RT-PCR. Sequencing the RT-PCR products synthesized from double-stranded RNA confirmed the presence of the chimeric segment used for tagging. This is the first report of an infectious cDNA molecule derived from CTV D-RNA.", }