1887

Abstract

A naturally occurring defective RNA of 2379 nt (D2.3) from the VT strain of citrus tristeza clostero- virus (CTV) was cloned and sequenced. The D2.3 RNA is a fusion of two regions of 1521 and 858 nt from the 5′ and 3′ ends of the CTV genome, respectively. A cDNA clone of D2.3 RNA was tagged by the insertion of a 0·47 kb chimeric DNA fragment and the recombinant cDNA was inserted downstream of the cauliflower mosaic virus 35S promoter. The resulting construct was bombarded into CTV-infected tissue, which was then grafted onto virus-free plants. The presence of recombinant RNA in systemically infected leaves was demonstrated by RT-PCR. Sequencing the RT-PCR products synthesized from double-stranded RNA confirmed the presence of the chimeric segment used for tagging. This is the first report of an infectious cDNA molecule derived from CTV D-RNA.

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1997-07-01
2022-08-16
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