1887

Abstract

The importance of VP7 of bluetongue virus (BTV) in the binding of BTV to membrane proteins of the BTV vector was investigated. Core BTV particles, prepared from whole viruses, lacked outer proteins VP2 and VP5 and had VP7 exposed. More core particles and whole viruses bound to membrane preparations of adults of and KC cells, which were cultured from this vector insect, than to membrane preparations of larvae. More core particles than whole viruses bound to membrane preparations of adults of and KC cells. Polyclonal anti-idiotypic antibodies (anti-Id), which were made against an antigen-combining region of an anti-BTV-10 VP7 antibody and functionally mimicked VP7, bound more to the membrane preparations of adults of and KC cells, and less to cytosol preparations. In Western overlay analysis, the plasma membrane preparation reduced binding of an anti-VP7 monoclonal antibody to VP7. Whole and core BTV particles and the anti-Id bound to a membrane protein with a molecular mass of 23 kDa that was present predominantly in membrane preparations of adults of and KC cells. This protein was present in much lower concentrations in membrane preparations of C6/36 and DM2 insect cells.

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1997-07-01
2022-05-23
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