1887

Abstract

The importance of VP7 of bluetongue virus (BTV) in the binding of BTV to membrane proteins of the BTV vector was investigated. Core BTV particles, prepared from whole viruses, lacked outer proteins VP2 and VP5 and had VP7 exposed. More core particles and whole viruses bound to membrane preparations of adults of and KC cells, which were cultured from this vector insect, than to membrane preparations of larvae. More core particles than whole viruses bound to membrane preparations of adults of and KC cells. Polyclonal anti-idiotypic antibodies (anti-Id), which were made against an antigen-combining region of an anti-BTV-10 VP7 antibody and functionally mimicked VP7, bound more to the membrane preparations of adults of and KC cells, and less to cytosol preparations. In Western overlay analysis, the plasma membrane preparation reduced binding of an anti-VP7 monoclonal antibody to VP7. Whole and core BTV particles and the anti-Id bound to a membrane protein with a molecular mass of 23 kDa that was present predominantly in membrane preparations of adults of and KC cells. This protein was present in much lower concentrations in membrane preparations of C6/36 and DM2 insect cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-7-1617
1997-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/7/9225038.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-7-1617&mimeType=html&fmt=ahah

References

  1. Anderson R. M., May R. M. 1991 Infectious Diseases of Humans : Dynamics and Control Oxford: Oxford University Press;
    [Google Scholar]
  2. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 92:248–254
    [Google Scholar]
  3. Brookes S. M., Hyatt A. D., Eaton B. T. 1994; The use of immuno- gold staining in bluetongue virus adsorption and neutralisation studies. Journal of Virological Methods 46:117–132
    [Google Scholar]
  4. Cowley J. A., Gorman B. M. 1986; Use of genetic reassortants to determine the genes encoding various biological properties ofbluetongue viruses. In Arbovirus Research in Australia, Proceedings ofthe 4th Symposium pp 266–271 CSIRO, Division of Tropical Animal Production, Indooroopily; Queensland, Australia:
    [Google Scholar]
  5. Cowley J. A., Gorman B. M. 1989; Cross-neutralisation of genetic reassortants of bluetongue virus serotypes 20 and 21. Veterinary Microbiology 19:37–51
    [Google Scholar]
  6. Graham G. 1984; Isolation of subcellular organelles and membranes. In Centrifugation : A Practical Approach, 2nd edn.. pp 161–182 Rickwood D. Edited by Oxford: IRL Press;
    [Google Scholar]
  7. Grimes J., Basak A. K., Roy P., Stuart D. 1995; The crystal structure of bluetongue virus VP7. Nature 373:167–170
    [Google Scholar]
  8. Hewat E. A., Booth T. F., Loudon P. T., Roy P. 1992; Structure of bluetongue virus particle by cryo-electron microscopy. Journal of Structural Biology 109:61–69
    [Google Scholar]
  9. Holmes I. H., Boccardo G., Estes M. K., Furuichi Y., Hoshino M. R., Joklik W. K., McCrae M., Mertens P. P. C., Milne R. G., Shikata R., Winton J. R. 1995; Family Reoviridae. In Virus Taxonomy. Sixth Report ofthe International Committee on Taxonomy of Viruses pp 208–239 Murphy F. A., Fauquet C. M., Bishop D. H. L., Ghabrial S. A., Jarvis A. W., Martinelli G. P., Mayo M. A., Summers M. D. Edited by Vienna & New York: Springer-Verlag;
    [Google Scholar]
  10. Huismans H., Van Der Walt N. T., Cloete M., Erasmus B. J. 1983; The biochemical and immunological characterization of bluetongue virus outer capsid polypeptides. In dsRNA Viruses pp 165–172 Compans R. W., Bishop D. H. L. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  11. Huismans H., Van Dijk A. A., Bauskin A. R. 1987a; In vitro phosphorylation and purification of a nonstructural protein of bluetongue virus with affinity for single-stranded RNA. Journal of Virology 61:3589–3595
    [Google Scholar]
  12. Huismans H., Van Der Walt N. T., Cloete M., Erasmus B. J. 1987b; Isolation of a capsid protein of bluetongue virus that induces a protective immune response in sheep. Virology 157:172–179
    [Google Scholar]
  13. Huismans H., Van Dijk A. A., Els H. J. 1987c; Uncoating of parental bluetongue virus to core and subcore particles in infected cells. Virology 157:180–188
    [Google Scholar]
  14. Hyatt A. D., Eaton B. T. 1988; Ultrastructural distribution of the major capsid proteins within bluetongue virus and infected cells. Journal of General Virology 69:805–815
    [Google Scholar]
  15. Jones R. H., Foster N. M. 1978; Heterogeneity of Culicoides variipennis field populations to oral infection with bluetongue virus. American Journal of Tropical Medicine and Hygiene 27:178–183
    [Google Scholar]
  16. Jones R. H., Luedke A. J., Walton T. E., Metcalf H. E. 1981; Bluetongue in the United States, an entomological perspective toward control. World Animal Review 38:2–8
    [Google Scholar]
  17. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  18. Lewis S. A., Grubman M. J. 1990; Bluetongue virus: surface exposure of VP7. Virus Research 16:17–26
    [Google Scholar]
  19. Ludwig G. V., Kondig J. P., Smith J. F. 1996; A putative receptor for Venezuelan equine encephalitis virus from mosquito cells. Journal of Virology 70:5592–5599
    [Google Scholar]
  20. Marchi P. R., Rawlings P., Burroughs J. N., Wellby M., Mertens P. P. C., Mellor P. S., Wade-Evans A. M. 1995; Proteolytic cleavage of VP2, an outer capsid protein of African horse sickness virus, by species-specific serum proteases enhances infectivity in Culicoides. Journal of General Virology 76:2607–2611
    [Google Scholar]
  21. Mecham J. O., Nunamaker R. A. 1994; Complex interactions between vectors and pathogens: Culicoides variipennis sonorensis (Diptera: Ceratopogonidae) infection rates with bluetongue viruses. Journal of Medical Entomology 31:903–907
    [Google Scholar]
  22. Mecham J. O., Dean V. C., Jochim M. M. 1986; Correlation of serotype specificity and protein structure of the five U. S. serotypes of bluetongue virus. Journal of General Virology 67:2617–2624
    [Google Scholar]
  23. Mecham J. O., Dean V. C., Wigington J. G., Nunamaker R. A. 1990; Detection of bluetongue virus in Culicoides variipennis (Diptera : Ceratopogonidae) by an antigen capture enzyme-linked immunosorbent assay. Journal of Medical Entomology 27:602–606
    [Google Scholar]
  24. Mellor P. S. 1990; The replication of bluetongue virus in Culicoides vectors. Current Topics in Microbiology and Immunology 162:143–161
    [Google Scholar]
  25. Mertens P. P. C., Burroughs J. N., Anderson J. 1987; Purification and properties of virus particles, infectious subviral particles, and cores of bluetongue virus serotypes 1 and 4. Virology 157:375–386
    [Google Scholar]
  26. Mertens P. P. C., Pedley S., Cowley J., Burroughs J. N., Corteyn A. H., Jeggo M. H., Jennings D. M., Gorman B. M. 1989; Analysis of the roles of the bluetongue virus outer coat proteins VP2 and VP5 in determination of virus serotype. Virology 70:561–565
    [Google Scholar]
  27. Mertens P. P. C., Burroughs J. N., Walton A., Wellby M. P., Fu H., O’Hara R. S., Brookes S. M., Mellor P. S. 1996; Enhanced infectivity of modified bluetongue virus particles for two insect cell lines and for two Culicoides vector species. Virology 217:582–593
    [Google Scholar]
  28. Roy P. 1992; Bluetongue virus proteins. Journal ofGeneral Virology 73:3051–3064
    [Google Scholar]
  29. Roy P., Marshall J. J. A., French T. J. 1990; Structure of the bluetongue virus genome and its encoded proteins. In Bluetongue Virus pp 43–87 Roy P., Gorman B. M. Edited by Heidelberg: Springer;
    [Google Scholar]
  30. Sellers R. F. 1981; Bluetong ue and related diseases. In Virus Diseases of Food Animals 2 pp 567–584 Gibbs E. P. J. Edited by London: Academic Press;
    [Google Scholar]
  31. Tabachnick W. J. 1996; Culicoides variipennis and bluetongue virus epidemiology in the United States. Annual Review of Entomology 41:23–43
    [Google Scholar]
  32. Tabachnick W. J., Holbrook F. R. 1992; The Culicoides variipennis complex and the distribution of the bluetongue viruses in the United States. In Proceedings of the 96th Annual Meeting ofthe United States Animal Health Association (USAHA) pp 207–212 Richmond, Va.; USAHA.
    [Google Scholar]
  33. Verwoerd D. W., Els H. J., De Villiers E.-M., Huismans H. 1972; Structure of the bluetongue virus capsid. Journal of Virology 10:783–794
    [Google Scholar]
  34. Walton T. E., Tabachnick W. J., Thompson L. H., Holbrook F. R. 1992; An entomologic and epidemiologic perspective for bluetongue (BLU) regulatory changes for livestock movement from the United States and observations on BLU in the Caribbean Basin. In Bluetongue, African Horse Sickness & Related Orbiviruses pp 952–960 Walton T. E., Osburn B. I. Edited by Boca Raton, Fla: CRC Press;
    [Google Scholar]
  35. Wechsler S. J., McHolland L. E., Wilson W. C. 1991; A RNA virus in cells from Culicoides variipennis. Journal of Invertebrate Pathology 57:200–205
    [Google Scholar]
  36. Zhou E. M., Huang W. 1995; Anti-idiotypic antibody as potential serodiagnostic reagent for detection of bluetongue virus infection. Journal of Clinical Microbiology 33:850–854
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-7-1617
Loading
/content/journal/jgv/10.1099/0022-1317-78-7-1617
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error