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Journal of General Virology header logo Journal of General Virology

Volume 78, Issue 6

Identification and functional analysis of a non-hr origin of DNA replication in the genome of Spodoptera exigua multicapsid nucleopolyhedrovirus No Access

Abstract

The genome of multicapsid nucleopolyhedrovirus (SeMNPV) was screened for the presence of putative origins of DNA replication (s). Using a transient DNA replication assay, several fragments were identified that underwent SeMNPV-dependent DNA replication in cells (Sf-AE-21). Preliminary sequence data revealed the presence of multiple copies of homologous repeats (s). Restriction fragment I-F2 showed a distinct sequence reminiscent of and MNPV (AcMNPV and OpMNPV) non-fir oris. Deletion analysis of this fragment indicated that the essential sequences of this putative non- mapped within a region of 800 bp. Sequence analysis of this region showed a unique distribution of six different (im)-perfect palindromes, several polyadenylation motifs and the occurrence of multiple direct repeats. No sequence homology or similarities to other reported baculovirus were detected. The spatial and modular distribution of these motifs are similar to those of the non- of AcMNPV and OpMNPV. Comparison of baculovirus non-fir and consensus eukaryotic revealed no consensus but indicated that each of the non-s studied so far is unique. From the structural similarity, however, it was concluded that the SeMNPV I-F2 represents a baculovirus non-fir type . In addition, evidence is provided that SeMNPV renders more specificity to baculovirus DNA replication than AcMNPV.

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1997-06-01
2025-07-14
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/content/journal/jgv/10.1099/0022-1317-78-6-1497
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Identification and functional analysis of a non-hr origin of DNA replication in the genome of Spodoptera exigua multicapsid nucleopolyhedrovirus
J. Gen. Virol. 78, 1497 (1997); https://doi.org/10.1099/0022-1317-78-6-1497
/content/journal/jgv/10.1099/0022-1317-78-6-1497
/content/journal/jgv/10.1099/0022-1317-78-6-1497
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