1887

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Volume 78, Issue 6

Direct demonstration of persistent Epstein-Barr virus gene expression in peripheral blood of infected common marmosets and analysis of virus-infected tissues in vivo Free

Abstract

Epstein-Barr virus (EBV) infection in animal model systems has been studied previously in marmosets and tamarins using serology and PCR of saliva. Here we directly demonstrated long-term persistence of EBV in the peripheral blood of marmosets by assaying EBER RNA expression. A new reverse transcription-PCR assay, able to distinguish a naturally occurring strain polymorphism in EBER 2 that may be useful as a strain marker for monitoring persistence and interactions between multiple strains in the same animal or person, has been developed. hybridization and immunohisto-chemistry have also been used to search for EBV-infected cells in the animals. The carrier state in the common marmoset is similar to that of humans in that it is asymptomatic, long-lived and displays a very low level of circulating virus-infected cells. It differs from the human in lacking the characteristic antibody response to EBNA 1.

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© Society for General Microbiology 1997
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1997-06-01
2024-04-20
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References

  1. Arrand J. R., Young L. S., Tugwood J. D. 1989; Two families of sequences in the small RNA-encoding region of Epstein-Barr virus (EBV) correlate with EBV types A and B. Journal of Virology 63:983–986
     [Google Scholar]
  2. Baer R., Bankier A. T., Biggin M. D., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. S., Satchwell S. C., Seguin C., Tufnell P. S., Barrell B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310:207–211
     [Google Scholar]
  3. Banerjee S., Livanos E., Vos J. H. 1995; Therapeutic gene delivery in human B-lymphoblastoid cells by engineered non-transforming infectious Epstein-Barr virus. Nature Medicine 1:1303–1308
     [Google Scholar]
  4. Cox C., Chang S., Karran L., Griffin B., Wedderburn N. 1996; Persistent Epstein-Barr virus infection in the common marmoset (Callithrix jacchus). Journal of General Virology 77:1173–1180
     [Google Scholar]
  5. Decker L. L., Klaman L. D., Thorley-Lawson D. A. 1996; Detection of the latent form of Epstein-Barr virus DNA in the peripheral blood of healthy individuals. Journal of Virology 70:3286–3289
     [Google Scholar]
  6. Desgranges C., Lenoir G., de The G., Seigneurin J. M., Hilgers J., Dubouch P. 1976; In vitro transforming activity of EBV. I- Establishment and properties of two EBV strains (M81 and M72) producedby immortalized Callithrix jacchus lymphocytes. Biomedicine 25:349–352
     [Google Scholar]
  7. de The G., Dubouch P., Fontaine C., Wedderburn N., Carter R. L., Edwards M. B., Cohen B. 1980; Natural antibodies to EBV-VCA antigens in common marmosets (Callithrix jacchus) and response after EBV inoculation. Intervirology 14:284–291
     [Google Scholar]
  8. Emini E. A., Luka J., Armstrong M. E., Banker F. S., Provost P. J., Pearson G. R. 1986; Establishment and characterization of a chronic infectious mononucleosis-like syndrome in common marmosets. Journal of Medical Virology 18:369–379
     [Google Scholar]
  9. Evans T. J., Brooks L., Farrell P. J. 1997; A strategy for specific targeting of therapeutic agents to tumour cells of virus-associated cancers. Gene Therapy 4:264–267
     [Google Scholar]
  10. Franken M., Estabrooks A., Cavacini L., Sherburne B., Wang F., Scadden D. T. 1996; Epstein-Barr virus-driven gene therapy for EBV-related lymphomas. Nature Medicine 2:1379–1382
     [Google Scholar]
  11. Greenspan D., Greenspan J. S., Conart M., Petersen V., Silverman S., de Souza Y. 1985; Replication of Epstein-Barr virus within the epithelial cells of oral ‘hairy’ leukoplakia, an AIDS associated lesion. New England Journal of Medicine 313:1564–1571
     [Google Scholar]
  12. Gutierrez M. I., Judde J. G., Magrath I. T., Bhatia K. G. 1996; Switching viral latency to viral lysis: a novel therapeutic approach for Epstein-Barr virus-associated neoplasia. Cancer Research 56:969–972
     [Google Scholar]
  13. Kempkes B., Pich D., Zeidler R., Sugden B., Hammerschmidt W. 1995; Immortalization of human B lymphocytes by a plasmid containing 71 kilobase pairs of Epstein-Barr virus DNA. Journal of Virology 69:231–238
     [Google Scholar]
  14. Khan G., Miyashita E. M., Yang B., Babcock G. J., Thorley-Lawson D. A. 1996; Is EBV persistence in vivo a model for B cell homeostasis?. Immunity 5:173–180
     [Google Scholar]
  15. Levitskaya J., Coram M., Levitsky V., Imreh S., Steigerwald-Mullen P. M., Klein G., Kurilla M. G., Masucci M. G. 1995; Inhibition of antigen processing by the internal repeat region ofthe Epstein-Barr virus nuclear antigen-1. Nature 375:685–688
     [Google Scholar]
  16. Mackett M., Cox C., Pepper S. de V., Lees J. F., Naylor B. A., Wedderburn N., Arrand J. R. 1996; Immunisation of common marmosets with vaccinia virus expressing Epstein-Barr virus (EBV) gp340 and challenge with EBV. Journal of Medical Virology 50:263–271
     [Google Scholar]
  17. Miyashita E. M., Yang B., Lam K. M., Crawford D. H., Thorley-Lawson D. A. 1995; A novel form of Epstein-Barr virus latency in normal B cells in vivo. Cell 80:593–601
     [Google Scholar]
  18. Niedobitek G., Young L. S. 1994; Epstein-Barr virus persistence and virus-associated tumours. Lancet 343:333–335
     [Google Scholar]
  19. Niedobitek G., Young L. S., Lau R., Brooks L., Greenspan D., Greenspan J. S., Rickinson A. B. 1991; Epstein-Barr virus infection in oral hairy leukoplakia: virus replication in the absence of a detectable latent phase. Journal of General Virology 72:3035–3046
     [Google Scholar]
  20. Niedobitek G., Herbst H., Young L. S., Brooks L., Masucci M. G., Crocker J., Rickinson A. B., Stein H. 1992; Patterns of Epstein-Barr virus infection in non-neoplastic lymphoid tissue. Blood 79:2520–2526
     [Google Scholar]
  21. Niedobitek G., Agathanggelou A., Finerty S., Tierney R., Watkins P., Jones E. L., Morgan A., Young L. S., Rooney N. 1994; Latent Epstein-Barr virus infection in cottontop tamarins. A possible model for Epstein-Barr virus infection in humans. American Journal ofPathology 145:969–978
     [Google Scholar]
  22. Qu L., Rowe D. T. 1992; Epstein-Barr virus latent gene expression in uncultured peripheral blood lymphocytes. Journal of Virology 66:3715–3724
     [Google Scholar]
  23. Rickinson A. B., Kieff E. 1996; Epstein -Barr virus. In Fields Virology, 3rd edn. pp. 2397–2446 Fields B. N., Knipe D. M., Howley P. M. Edited by Philadelphia: Lippincott-Raven;
     [Google Scholar]
  24. Robertson E. S., Ooka T., Kieff E. D. 1996; Epstein -Barr virus vectors for gene delivery to B lymphocytes. Proceedings of the National Academy of Sciences, USA 93:11334–11340
     [Google Scholar]
  25. Rowe M., Evans H. S., Young L. S., Hennessy K., Kieff E., Rickinson A. B. 1987; Monoclonal antibodies to the latent membrane protein of Epstein-Barr virus reveal heterogeneity of the protein and inducible expression in virus-transformed cells. Journal of General Virology 68:1575–1586
     [Google Scholar]
  26. Tierney R. J., Steven N., Young L. S., Rickinson A. B. 1994; Epstein-Barr virus latency in blood mononuclear cells : analysis of viral gene transcription during primary infection and in the carrier state. Journal of Virology 68:7374–7385
     [Google Scholar]
  27. Watkins D. I., Chen Z. W., Hughes A. L., Hodi F. S., Letvin N. L. 1990; Genetically distinct cell populations in naturally occurring bone marrow chimaeric primates express similar MHC class I gene products. Journal of Immunology 144:3726–3735
     [Google Scholar]
  28. Wedderburn N., Edwards J. M., Desgranges C., Fontaine C., Cohen B., de The G. 1984; Infectious mononucleosis-like response in common marmosets infected with Epstein-Barr virus. Journal of Infectious Diseases 150:878–882
     [Google Scholar]
  29. Yao Q. Y., Rickinson A. B., Epstein M. A. 1985; A re-examination of the Epstein-Barr virus carrier state in healthy seropositive individuals. International Journal of Cancer 35:35–42
     [Google Scholar]
  30. Young L., Alfieri C., Hennessy K., Evans H., O’Hara C., Anderson K. C., Ritz J., Shapiro R. S., Rickinson A., Kieff E., Cohen J. I. 1989; Expression of Epstein-Barr virus transformation-associated genes in tissues of patients with EBV lymphoproliferative disease. New England Journal of Medicine 321:1080–1085
     [Google Scholar]
  31. Young L. S., Lau R., Rowe M., Niedobitek G., Packham G., Shanahan F., Rowe D. T., Greenspan D., Greenspan J. S., Rickinson A. B., Farrell P. J. 1991; Differentiation-associated expression ofthe Epstein-Barr virus BZLF1 transactivator protein in oral hairy leukoplakia. Journal of Virology 65:2868–2874
     [Google Scholar]
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Direct demonstration of persistent Epstein-Barr virus gene expression in peripheral blood of infected common marmosets and analysis of virus-infected tissues in vivo
J. Gen. Virol. 78, 1417 (1997); https://doi.org/10.1099/0022-1317-78-6-1417
/content/journal/jgv/10.1099/0022-1317-78-6-1417
/content/journal/jgv/10.1099/0022-1317-78-6-1417
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