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Journal of General Virology header logo Journal of General Virology

Volume 78, Issue 6

Direct demonstration of persistent Epstein-Barr virus gene expression in peripheral blood of infected common marmosets and analysis of virus-infected tissues in vivo No Access

Abstract

Epstein-Barr virus (EBV) infection in animal model systems has been studied previously in marmosets and tamarins using serology and PCR of saliva. Here we directly demonstrated long-term persistence of EBV in the peripheral blood of marmosets by assaying EBER RNA expression. A new reverse transcription-PCR assay, able to distinguish a naturally occurring strain polymorphism in EBER 2 that may be useful as a strain marker for monitoring persistence and interactions between multiple strains in the same animal or person, has been developed. hybridization and immunohisto-chemistry have also been used to search for EBV-infected cells in the animals. The carrier state in the common marmoset is similar to that of humans in that it is asymptomatic, long-lived and displays a very low level of circulating virus-infected cells. It differs from the human in lacking the characteristic antibody response to EBNA 1.

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© Society for General Microbiology 1997
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1997-06-01
2025-03-15
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/content/journal/jgv/10.1099/0022-1317-78-6-1417
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Direct demonstration of persistent Epstein-Barr virus gene expression in peripheral blood of infected common marmosets and analysis of virus-infected tissues in vivo
J. Gen. Virol. 78, 1417 (1997); https://doi.org/10.1099/0022-1317-78-6-1417
/content/journal/jgv/10.1099/0022-1317-78-6-1417
/content/journal/jgv/10.1099/0022-1317-78-6-1417
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