1887

Abstract

We have previously demonstrated that two distinct transcripts are produced from ORF 50, the major transcriptional activating gene of herpesvirus saimiri. The products of these transcripts trans- activate the delayed-early ORF 6 promoter, though to different degrees. Deletion analysis demonstrated that the ORF 50 responsive elements are contained in a 132 bp fragment situated 127–259 bp from the transcription initiation site within the ORF 6 promoter. This fragment conferred ORF 50-responsiveness on an enhancerless simian virus 40. Gel retardation analysis further mapped the responsive elements to a 38 bp fragment.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-6-1411
1997-06-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/6/9191938.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-6-1411&mimeType=html&fmt=ahah

References

  1. Albrecht J. C., Fleckenstein B. 1990; Structural organization of the conserved gene block of herpesvirus saimiri coding for DNA polymerase, glycoprotein B, and major DNA binding protein. Virology 174:533–542
    [Google Scholar]
  2. Albrecht J. C., Nicholas J., Biller D., Cameron K. R., Biesinger B., Newman C., Wittman S., Craxton M. A., Coleman H., Fleckenstein B., Honess R. W. 1992; Primary structure of the herpesvirus saimiri genome. Journal of Virology 66:5047–5058
    [Google Scholar]
  3. Andrews N. C., Faller D. V. 1991; A rapid micropreparation technique for extraction of DNA-binding proteins from limiting numbers of mammalian cells. Nucleic Acids Research 19:2499
    [Google Scholar]
  4. Bublot M., Manet E., Lequarre A. S., Albrecht J. C., Nicholas J., Fleckenstein B., Pastoret P. P., Thiry E. 1992; Genetic relationships between bovine herpesvirus 4 and the gamma-herpesviruses Epstein-Barr and herpesvirus saimiri. Virology 190:654–665
    [Google Scholar]
  5. Buisson M., Manet E., Trescol-Biemont M.-C., Gruffat H., Durand B., Sergeant A. 1989; The Epstein-Barr virus (EBV) early protein EB2 is a post transcriptional activator expressed under the control of EBV transcription factors EB1 and R. Journal of Virology 63:5276–5284
    [Google Scholar]
  6. Fleckenstein B., Desrosiers R. C. 1982; Herpesvirus saimiri and herpesvirus ateles. In The Herpesviruses 1 pp. 253–332 Roizman B. Edited by New York: Plenum Press;
    [Google Scholar]
  7. Furnari F. B., Zacny V., Quinlivan E. B., Kenney S., Pagano J. S. 1994; RAZ, an Epstein-Barr virus transdominant repressor that modulates the viral reactivation mechanism. Journal of Virology 68:1827–1836
    [Google Scholar]
  8. Gompels U. A., Craxton M. A., Honess R. W. 1988a; Conservation of gene organization in the lymphotropic herpesvirus saimiri. Journal of Virology 62:757–767
    [Google Scholar]
  9. Gompels U. A., Craxton M. A., Honess R. W. 1988b; Conservation of glycoprotein H (gH) in herpesvirus : nucleotide sequence of the gH gene from herpesvirus saimiri. Journal ofGeneral Virology 69:2819–2829
    [Google Scholar]
  10. Gorman C. M., Moffat L. F., Howard B. H. 1982; Recombinant genomes which express chloramphenicol acetyl-transferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  11. Gruffat H., Sergeant A. 1994; Characterization of the DNA-binding site repertoire for the Epstein-Barr virus transcription factor R. Nucleic Acids Research 22:1172–1178
    [Google Scholar]
  12. Gruffat H., Manet E., Rigolet A., Sergeant A. 1990; The enhancer factor R of Epstein-Barr virus (EBV) is a sequence specific DNA binding protein. Nucleic Acids Research 18:6835–6843
    [Google Scholar]
  13. Gruffat H., Duran N., Buisson M., Wild F., Buckland R., Sergeant A. 1992; Characterization of an R-binding site mediating the R-induced activation of the Epstein-Barr virus BMLF1 promoter. Journal of Virology 66:46–52
    [Google Scholar]
  14. Kenney S., Holley-Guthrie E. A., Mar E.-C., Smith M. 1989; The Epstein-Barr virus BMLF1 promoter contains an enhancer element that is responsive to the BZLF1 and BRLF1 fransactivators. Journal of Virology 63:3878–3883
    [Google Scholar]
  15. Knust E., Schirm S., Dietrich W., Bodemer W., Kolb E., Fleckenstein B. 1983; Cloning of the herpesvirus saimiri DNA fragments representing the entire L-region of the genome. Gene 25:281–289
    [Google Scholar]
  16. Nicholas J., Cameron K. R., Coleman H., Newman C., Honess R. W. 1992; Analysis of nucleotide sequence of the rightmost 43 kbp of herpesvirus saimiri (HVS) L-DNA: general conservation of genetic organization between HVS and Epstein-Barr virus. Virology 188:296–310
    [Google Scholar]
  17. Nicholas J., Coles L. S., Newman C., Honess R. W. 1991; Regulation of the herpesvirus saimiri (HVS) delayed-early 110-kilodalton promoter by HVS immediate-early gene products and a homolog of the Epstein-Barr virus R transactivator. Journal of Virology 65:2457–2466
    [Google Scholar]
  18. Rooney C. M., Rowe D. T., Ragot T., Farrell P. J. 1989; The spliced BZLF1 gene of Epstein-Barr virus (EBV) transactivates an early EBV promoter and induces the virus production cycle. Journal of Virology 63:3109–3116
    [Google Scholar]
  19. Whitehouse A., Carr I., Griffiths J. C., Meredith D. M. 1997; The herpesvirus saimiri ORF 50 gene, encoding a major transcriptional activator homologous to the Epstein-Barr Virus R protein, is transcribed from two distinct promoters of different temporal phases. Journal of Virology 71:2550–2554
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-6-1411
Loading
/content/journal/jgv/10.1099/0022-1317-78-6-1411
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error