1887

Abstract

Two monoclonal antibody neutralization resistant (MAb) variants of the yellow fever (YF) 17D-204 vaccine virus strain were selected using YF type- specific MAb B39. These B39 variants were compared with the variant virus selected by Lobigs 161,474–478, 1987) using a second YF- type specific MAb (2E10) which mapped to amino acid position 71/72 in the envelope (E) protein. Neutralization assays with a panel of MAbs suggested that these two YF-type-specific epitopes are located in two discrete regions of the folded E protein. Each of the B39 variants had a single nucleotide mutation which encoded an amino acid substitution at either position E-155 or E-158. Thus, YF type-specific epitopes map to both domain I (B39) and II (2E10) of the YF virus E protein. The B39 defined epitope represents the first flavivirus neutralizing epitope localized to this region of domain I of the E protein.

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1997-06-01
2024-04-18
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References

  1. Barrett A. D. T., Pryde A., Medlen A. R., Ledger T. N., Whitby J. E., Gibson C. A., DeSilva M., Groves D. J., Langley D. J., Minor P. D. 1989; Examination of the envelope glycoprotein of yellow fever vaccine viruses with monoclonal antibodies. Vaccine 7:333–336
    [Google Scholar]
  2. Cecilia D., Gould E. A. 1991; Nucleotide changes responsible for loss of neuroinvasiveness in Japanese encephalitis virus neutralization-resistant mutants. Virology 181:70–77
    [Google Scholar]
  3. Chambers T. J., Hahn C. S., Galler R., Rice C. M. 1990; Flavivirus genome organisation, expression and replication. Annual Review of Microbiology 44:649–688
    [Google Scholar]
  4. Guirakhoo F., Heinz F. X., Kunz C. 1989; Epitope model of tick-borne encephalitis virus envelope glycoprotein E: analysis of structural properties, role of carbohydrate side-chain, and conformational changes occurring at acidic pH. Virology 169:90–99
    [Google Scholar]
  5. Hasegawa H., Yoshida M., Shiosaka T., Fujita S., Kobayashi Y. 1992; Mutations in the envelope protein of Japanese encephalitis virus affect entry into cultured cells and virulence in mice. Virology 191:158–165
    [Google Scholar]
  6. Heinz F. X., Mandl C. W. 1993; The molecular biology of tick-borne encephalitis virus. Acta Pathologica Microbiologica et Immunologica Scandinavica 101:735–745
    [Google Scholar]
  7. Holzmann H., Heinz F. X., Mandl C. W., Guirakhoo F., Kunz C. 1990; A single amino acid substitution in the envelope protein E of tick-borne encephalitis virus leads to attenuation in the mouse model. Journal of Virology 64:5156–5159
    [Google Scholar]
  8. Jennings A. D., Whitby J. E., Minor P. D., Barrett A. D. T. 1993; Comparison of the nucleotide and deduced amino acid sequences of the structural protein genes of the yellow fever 17DD vaccine strain from Senegal with those of other yellow fever vaccine viruses. Vaccine 11:679–681
    [Google Scholar]
  9. Jiang W. R., Lowe A., Higgs S., Reid H., Gould E. A. 1993; Single amino acid codon changes detected in louping ill virus antibody resistant mutants with reduced neurovirulence. Journal of General Virology 74:931–935
    [Google Scholar]
  10. Johnson A. J., Guirakhoo F., Roehrig J. T. 1994; The envelope glycoproteins of dengue 1 and dengue 2 viruses grown in mosquito cells differ in their utilization of potential glycosylation sites. Virology 203:241–249
    [Google Scholar]
  11. Kawano H., Rostapshov V., Rosen L., Lai C.-J. 1993; Genetic determinants of dengue type 4 virus neurovirulence for mice. Journal of Virology 67:6567–6575
    [Google Scholar]
  12. Ledger T. N., Sil B. K., Wills M. R., Lewis G., Kinney R. M., Jennings A. D., Stephenson J. R., Barrett A. D. T. 1992; biological functions of envelope protein epitopes of yellow fever vaccine viruses detected with monoclonal antibodies. Biologicals 20:117–128
    [Google Scholar]
  13. Lin B., Parrish C. R., Murray J. M., Wright P. J. 1994; Localization of a neutralizing epitope on the envelope protein of dengue virus type 2. Virology 20:885–890
    [Google Scholar]
  14. Lobigs M., Dalgarno L., Schlesinger J. J., Weir R. E. 1987; Location of a neutralization determinant in the E protein of yellow fever virus (17D vaccine strain). Virology 161:474–478
    [Google Scholar]
  15. MacMinn P. C., Lee E., Hartley S., Roehrig J. T., Dalgarno L., Weir R. C. 1995; Murray Valley encephalitis virus envelope protein antigenic variants with altered hemagglutination properties and reduced neuroinvasiveness in mice. Virology 211:10–20
    [Google Scholar]
  16. Mandl C. W., Guirakhoo F., Holzmann H., Heinz F. X., Kunz C. 1989; Antigenic structure of the flavivirus envelope protein E at the molecular level using tick-borne encephalitis as a model. Journal of Virology 63:564–571
    [Google Scholar]
  17. Marin M. S., Zanotto P. M. de A., Gritsun T. S., Gould E. A. 1995; Phylogeny of TYU, SRE, and CFA virus: different evolutionary rates in the genus Flavivirus. Virology 206:1133–1139
    [Google Scholar]
  18. Monath T. P. 1986; Pathology of the flaviviruses. In The Togaviridae and Flaviviridae pp. 375–440 Schlesinger S., Schlesinger M. J. Edited by New York: Plenum Press;
    [Google Scholar]
  19. Pletnev A. G., Bray M., Huggins J., Lai C.-J. 1992; Construction and characterization of chimeric tick-borne encephalitis/dengue type 4 viruses. Proceedings of the National Academy of Sciences, USA 89:10532–10536
    [Google Scholar]
  20. Post P. R., Santos C. N. D., Carvalho R., Cruz A. C. R., Rice C. M., Galler R. 1992; Heterogeneity in envelope protein sequence and N-linked glycosylation among yellow fever virus vaccine strains. Virology 188:160–167
    [Google Scholar]
  21. Rey F. A., Heinz F. X., Mandl C. W., Kunz C., Harrison S. C. 1995; The envelope glycoprotein from tick-borne encephalitis virus at 2 Å resolution. Nature 375:291–298
    [Google Scholar]
  22. Rice C. M., Lenches E. M., Eddy S. R., Shih S. J., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus : implications for flavivirus gene expression and evolution. Science 229:726–733
    [Google Scholar]
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