RT Journal Article SR Electronic(1) A1 Nishio, Machiko A1 Tsurudome, Masato A1 Ito, Morihiro A1 Watanabe, Noriko A1 Kawano, Mitsuo A1 Komada, Hiroshi A1 Ito, YasuhikoYR 1997 T1 Human parainfluenza virus type 2 phosphoprotein: mapping of monoclonal antibody epitopes and location of the multimerization domain. JF Journal of General Virology, VO 78 IS 6 SP 1303 OP 1308 DO https://doi.org/10.1099/0022-1317-78-6-1303 PB Microbiology Society, SN 1465-2099, AB The epitopes recognized by 42 monoclonal antibodies directed against the human parainfluenza virus type 2 (hPIV-2) phosphoprotein (P) were mapped on the primary structure of the P protein by testing their reactivities with deletion mutants. By Western immunoblotting with these monoclonal antibodies and P protein deletion mutants the region essential for P-P interactions was determined. The P protein region encompassing amino acids 211–248 was required for proper folding and oligomerization which is mediated by predicted coiled-coils in this region. The oligomer was shown to be a homotrimer by chemical cross-linking experiments., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-78-6-1303