1887

Abstract

Three distinct genotypes were identified amongst 50 measles virus (MV) strains characterized in the UK between 1992 and 1995 by direct sequencing of the RT-PCR products amplified from clinical specimens. All three genotypes were related to viruses previously reported in the United States or in continental Europe. Phylogenetic analyses of 255 and 152 nucleotide sequences from the N and M genes, respectively, generated very similar lineages. The degree of divergence between genotypes was 3·5–16% and 2·6–7·9% in the N and the M genes, respectively. MV genotypes which circulated during the 1970s and 1980s in the UK were not detected in this period. Comparison of the C-terminal regions of the N gene sequences of UK strains isolated or detected between 1974 and 1995 suggests that there have been multiple genotypes of MV circulating in the UK over the past 20 years.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-6-1287
1997-06-01
2022-05-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/6/9191920.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-6-1287&mimeType=html&fmt=ahah

References

  1. Billeter M. A., Cattaneo R., Spielhofer P., Kaelin K., Huber M., Schmid A., Baczko K., Termeulen V. 1994; Generation and properties of measles virus mutations typically associated with subacute sclerosing panencephalitis. Slow Infections of the Central Nervous System 724:367–377
    [Google Scholar]
  2. Boom R., Sol C. J. A., Salimans H. M. M., Jansen C. L., Wertheim-van Dillen P. E. M., Van Der Noordaa J. 1990; Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology 28:495–503
    [Google Scholar]
  3. Buckland R., Giraldon P., Wild T. F. 1989; Expression of measles virus nucleoprotein in Escherichia coli: use of deletion mutants to locate the antigenic sites. Journal of General Virology 70:435–441
    [Google Scholar]
  4. Enders J. F., Peebles T. C. 1954; Propagation in tissue cultures of cytopathogenic agents from patients with measles. Proceedings of the Society for Experimental Biology and Medicine 86:277–286
    [Google Scholar]
  5. Forthal D. N., Blanding J., Aarnaes S., Peterson E. M., de la Maza L. M., Tilles J. G. 1993; Comparison of different methods and cell lines for isolating measles virus. Journal of Clinical Microbiology 31:695–697
    [Google Scholar]
  6. Giraudon P., Jacquier M. F., Wild T. F. 1988; Antigenic analysis of African measles virus field isolates: identification and localisation of one conserved and two variable epitope sites on the NP protein. Virus Research 18:137–152
    [Google Scholar]
  7. Jin L., Richards A., Brown D. W. G. 1996; Development of a dual target-PCR for detection and characterization of measles virus in clinical specimens. Molecular and Cellular Probes 10:191–200
    [Google Scholar]
  8. Kobune F., Sakata H., Sugiura A. 1990; Marmoset lymphoblastoid cells as a sensitive host for isolation of measles virus. Journal of General Virology 64:700–705
    [Google Scholar]
  9. Lecouturier V., Fayolle J., Caballero M., Carabana J., Celma M. L., Fernandez-Munoz R., Wild T. F., Buckland R. 1996; Identification of two amino acids in the hemagglutinin glycoprotein of measles virus (MV) that govern hemadsorption, HeLa cell fusion, and CD46 down-regulation: phenotypic markers that differentiate vaccine and wild-type MV strains. Journal of Virology 70:4200–4204
    [Google Scholar]
  10. Nakayama T., Mori T., Yamaguchi S., Sonoda S., Asamura S., Yamashita R., Takeuchi Y., Urano T. 1995; Detection of measles virus genome directly from clinical samples by reverse transcriptase- polymerase chain reaction and genetic variability. Virus Research 35:1–16
    [Google Scholar]
  11. Outlaw M. C., Pringle C. R. 1995; Sequence variation within an outbreak of measles virus in the Coventry area during spring/summer 1993. Virus Research 39:3–11
    [Google Scholar]
  12. Rima B. K., Earle J. A. P., Herlihy Y. L., Baczko K., ter Meulen V., Carabana J., Caballero M., Celma M. L., Fernandez-Munoz R. 1995; Temporal and geographical distribution of measles virus genotypes. Journal of General Virology 76:1173–1180
    [Google Scholar]
  13. Rota P. A., Bloom A. E., Vanchiere J. A., Bellini W. J. 1994; Evolution of the nucleoprotein and matrix genes of wild-type strains of measles virus isolated from recent epidemics. Virology 198:724–730
    [Google Scholar]
  14. Rota P. A., Rota J. S., Bellini W. J. 1995; Molecular epidemiology of measles virus. Seminars in Virology 6:379–386
    [Google Scholar]
  15. Rota J. S., Health J. L., Rota P. A., King G. E., Celma M. I., Carabana J., Fernandez-Munoz R., Brown D., Jin L., Bellini W. J. 1996; Molecular epidemiology of measles virus : identification of pathways of transmission and implications for measles elimination. Journal of Infectious Diseases 173:32–37
    [Google Scholar]
  16. Schmid A., Spielhofer P., Cattaneo R., Baczko K., ter Meulen V., Billeter M. A. 1992; Subacute sclerosing panencephalitis is typically characterized by alterations in the fusion protein cytoplasmic domain of the persisting measles virus. Virology 188:910–915
    [Google Scholar]
  17. Schulz T. F., Hoad J. G, Whitby D., Tizard E. J., Dillon M. J., Weiss R. A. 1992; A measles virus isolate from a child with Kawasaki disease: sequence comparison with contemporaneous isolates from ‘classical’ cases. Journal of General Virology 73:1581–1586
    [Google Scholar]
  18. Shibahara K., Hotta H., Katayama Y., Homma M. 1994; Increased binding activity of measles virus to monkey red blood cells after longterm passage in Vero cell cultures. Journal of General Virology 75:3511–3516
    [Google Scholar]
  19. Shimizu H., McCarthy C. A., Smaron M. F., Burns J. C. 1993; Polymerase chain reaction for detection of measles virus in clinical samples. Journal of Clinical Microbiology 31:1034–1039
    [Google Scholar]
  20. Taylor M. J., Godfrey E., Baczko K., ter Meulen V., Wild T. F., Rima B. K. 1991; Identification of several different lineages of measles virus. Journal of General Virology 72:83–88
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-6-1287
Loading
/content/journal/jgv/10.1099/0022-1317-78-6-1287
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error