1887

Abstract

Partial sequences from serologically characterized yam mosaic potyvirus (YMV) isolates were determined in conserved (helper-component proteinase, HC; nuclear inclusion b, NIb) and variable (first protein, P1; third protein, P3; and coat protein, CP) regions of the potyviral genome in order to investigate the intraspecies molecular diversity of YMV. Multiple sequence alignments and pairwise comparisons were used to quantify the sequence polymorphism in these regions. Two levels of diversity were observed among YMV isolates: above 90% nucleotide (nt) sequence identities were found between YMV isolates of the same group (intragroup) regardless of the region considered, whereas identities between isolates from different groups (intergroup) were lower and depended upon the protein chosen. For instance, the average intergroup nt sequence identity between YMV isolates was about 65% in the P1 protein and the N terminus of the CP while there was more than 80% nt identity in the HC, P3 and NIb proteins. Thus P3 appeared to be conserved between YMV isolates even though this region was variable between potyvirus species. Similar analysis of the intraspecies molecular diversity of other potyviruses (potato virus Y, zucchini yellow mosaic virus, plum pox virus, pea seed-borne mosaic virus) led to the same results: (i) two levels of intraspecies molecular diversity were found (intragroup and intergroup); (ii) intraspecies molecular diversity differed from interspecies molecular diversity in the P3, P1 and N- terminal regions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-6-1253
1997-06-01
2022-05-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/6/9191916.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-6-1253&mimeType=html&fmt=ahah

References

  1. Aleman M.-E., Marcos J. F., Brugidou C., Beachy R. N., Fauquet C. 1996; The complete nucleotide sequence of yam mosaic virus (Ivory Coast isolate). Archives of Virology 141:1259–1278
    [Google Scholar]
  2. Atreya P. L., Atreya C. D., Pirone T. P. 1991; Amino acid substitutions in the coat protein result in loss of insect transmissibility of a plant virus. Proceedings of the National Academy of Sciences, USA 88:7887–7891
    [Google Scholar]
  3. Baker C. A., Marlow G. C., Wisler G. W., Lecoq H., Hiebert E. 1994; Comparative sequence analysis of zucchini yellow mosaic potyvirus (ZYMV) from Reunion Island with other ZYMV isolates. EMBL accession number L31350
    [Google Scholar]
  4. Balint R., Plooy I., Steele C. 1990; The nucleotide sequence of zucchini yellow mosaic potyvirus. VIII International Congress of Virology Berlin: abstract 884–107
    [Google Scholar]
  5. Cervera M. T., Riechmann J. L., Martin M. T., Garcia J. A. 1993; 3′-terminal sequence of the plum pox virus PS and o6 isolates: evidence for RNA recombination within the potyvirus group. Journal of General Virology 74:329–334
    [Google Scholar]
  6. Cronin S., Verchot J., Haldeman-Cahill R., Schaad M. C., Carrington J. C. 1995; Long-distance movement factor: a transport function of the potyvirus helper component proteinase. Plant Cell 7:549–559
    [Google Scholar]
  7. Dayhoff M. 1978; Atlas of protein sequence and structure. National Biomedical Research Foundation 5: Suppl. 3
    [Google Scholar]
  8. Duterme O., Colinet D., Kummert J., Lepoivre P. 1996; Determination of the taxonomic position and characterization of yam mosaic virus isolates based on sequence data ofthe 5'-terminal part of the coat protein. Archives of Virology 141:1067–1075
    [Google Scholar]
  9. Fakhfakh H., Makni M., Robaglia C., Elgaaied A., Marrakchi M. 1995; Polymorphisme des regions capside et 3′NTR de 3 isolats tunisiens du virus Y de la pomme de terre (PVY). Agronomie 15:569–579
    [Google Scholar]
  10. Frenkel M. J., Ward C. W., Shukla D. D. 1989; The use of 3′ noncoding nucleotide sequences in the taxonomy of potyviruses: application to watermelon mosaic virus 2 and soybean mosaic virus-N. Journal of General Virology 70:2775–2783
    [Google Scholar]
  11. Goudou-Urbino C., Givord L., Quiot J. B., Boeglin M., Konate G., Dubern J. 1996; Differentiation of yam virus isolates using symptomatology, Western blot assay, and monoclonal antibodies. Journal of Phytopathology 144:235–240
    [Google Scholar]
  12. Habera L. F., Berger P. H., Reddick B. B. 1994; Molecular evidence from 3′-terminus sequence analysis that tobacco vein-banding mosaic virus is a distinct member of the potyvirus group. Archives of Virology 138:27–38
    [Google Scholar]
  13. Huet H., Gal-On A., Meir E., Lecoq H., Raccah B. 1994; Mutations in the helper component protease gene of zucchini yellow mosaic virus affect its ability to mediate aphid transmissibility. Journal of General Virology 75:1407–1414
    [Google Scholar]
  14. Johansen E., Rasmussen O. F., Heide M., Borkhardt B. 1991; The complete nucleotide sequence of pea seed-borne mosaic virus RNA. Journal of General Virology 72:2625–2632
    [Google Scholar]
  15. Johansen I. E., Keller K. E., Dougherty W. G., Hampton R. O. 1996; Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus. Journal of General Virology 77:1329–1333
    [Google Scholar]
  16. Koonin E. V. 1991; The phylogeny of RNA-dependent RNA polymerases of positive-strand RNA viruses. Journal of General Virology 72:2197–2206
    [Google Scholar]
  17. Lain S., Riechmann J.-L., Garcia J. A. 1989; The complete nucleotide sequence of plum pox potyvirus RNA. Virus Research 13:157–172
    [Google Scholar]
  18. Langeveld S. A., Dore J. M., Memelink J., Derks A. F. L. M., van der Vlugt C. I. M., Asje C. J., Bol J. F. 1991; Identification of potyviruses using the polymerase chain reaction with degenerate primers. Journal of General Virology 72:1531–1541
    [Google Scholar]
  19. Maiss E., Deborre G., Jelkmann W., Casper R. 1994; Complete nucleotide sequence of a plum pox potyvirus isolate (PVV-SC) deriving from sour cherries and influence of a coat protein sequence motif on aphid transmission. EMBL accession number X81083
    [Google Scholar]
  20. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H. D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology 70:513–524
    [Google Scholar]
  21. Palkovics L., Burgyan J., Balazs E. 1993; Comparative sequence analysis of four complete primary structures of plum pox virus strains. Virus Genes 7:339–347
    [Google Scholar]
  22. Quemada H., Sieu L. C., Siemieniak D. R., Gonsalves D., Slightom J. L. 1991; Watermelon mosaic virus II and zucchini yellow mosaic virus : cloning of 3′-terminal regions, nucleotide sequence, and phylogenetic comparisons. Journal of General Virology 71:1451–1460
    [Google Scholar]
  23. Revers F., Le Gall O., Candresse T., Le Romancer M., Dunez J. 1996; Frequent occurrence of recombinant potyvirus isolates. Journal of General Virology 77:1953–1965
    [Google Scholar]
  24. Riechmann J. L., Lain S., Garcia J. A. 1992; Highlights and prospects of potyvirus molecular biology. Journal of General Virology 73:1–16
    [Google Scholar]
  25. Robaglia C., Durand-Tardif M., Tronchet M., Boudazin G., Astier-Manifacier S., Casse-Delbart F. 1989; Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology 70:935–947
    [Google Scholar]
  26. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  27. Shukla D. D., Ward C. W. 1988; Amino acid sequence homology of coat proteins as a basis for identification and classification of the potyvirus group. Journal of General Virology 69:2703–2710
    [Google Scholar]
  28. Shukla D. D., Frenkel M. J., Ward C. W. 1991; Structure and function of the potyvirus genome with special reference to the coat protein coding region. Canadian Journal of Plant Pathology 13:178–191
    [Google Scholar]
  29. Shukla D. D., Ward C. W., Brunt A. A. 1994 The Potyviridae Wallingford: CAB International;
    [Google Scholar]
  30. Swofford D. L. 1992; PAUP: phylogenetic analysis using parsimony, version 3.0s. Center for Biodiversity, Illinois Natural History Survey Campaign:
    [Google Scholar]
  31. Teycheney P. Y., Tavert G., Delbos R., Ravelonandro M., Dunez J. 1989; The complete nucleotide sequence of plum pox virus RNA (strain D). Nucleic Acids Research 17:10115–10116
    [Google Scholar]
  32. Thole V., Dalmay T., Burgyan J., Balazs E. 1993; Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA. Gene 123:149–156
    [Google Scholar]
  33. Thouvenel J. C., Dumont R. 1990; Perte de rendement de l’igname infectée par le virus de la mosaique de l’igname en Côte d’Ivoire. L’Agronomie Tropicale 45:125–129
    [Google Scholar]
  34. Thouvenel J. C., Fauquet C. 1979; Yam mosaic, a new potyvirus infecting Dioscorea cayenensis in the Ivory Coast. Annals of Applied Biology 93:279–283
    [Google Scholar]
  35. Thouvenel J. C., Fauquet C. 1986; Yam mosaic virus. CMI/AAB Descriptions of Plant Viruses314
    [Google Scholar]
  36. Tordo V. M.-J., Chachulska A. M., Fakhfakh H., Romancer M. L., Robaglia C., Astier-Manifacier S. 1995; Sequence polymorphism in the 5′NTR and in the P1 coding region of potato virus Y genomic RNA. Journal of General Virology 76:939–949
    [Google Scholar]
  37. Verchot J. M., Herndon K. L., Carrington J. C. 1992; Mutational analysis of the tobacco etch potyviral 35-kDa proteinase: identification of essential residues and requirements for autoproteolysis. Virology 190:298–306
    [Google Scholar]
  38. Verwoerd T. C., Dekker B. M. M., Hoekema A. 1989; A small procedure for the rapid isolation of plant RNAs. Nucleic Acids Research 17:2362
    [Google Scholar]
  39. Ward C. W., Shukla D. D. 1994; Structure and variation of potyviruses. In Virology in the Tropics pp. 43–61 Rishi N., Ahuja K. L., Singh B. P. Edited by New Delhi: Malhotra Publishing House;
    [Google Scholar]
  40. Ward C. W., McKern N. M., Frenkel M. J., Shukla D. D. 1992; Sequence data as the major criterion for potyvirus classification. In Potyvirus Taxonomy Archives of Virology Supplement 5 pp. 283–297 Barnett O. W. Edited by Vienna & New York: Springer Verlag;
    [Google Scholar]
  41. Ward C. W., Weiller G. F., Shukla D. D., Gibbs A. 1995; Molecular systematics of the Potyviridae, the largest plant virus family. In Molecular Basis of Virus Evolution pp. 477–500 Gibbs A. J., Calisher C. H., Garcia-Arenal F. Edited by Cambridge: Press Syndicate of the University of Cambridge;
    [Google Scholar]
  42. Wetzel T., Candresse T., Ravelonandro M., Delbos R. P., Mazyad H., Aboul-Ata A. E., Dunez J. 1991; Nucleotide sequence of the 3′-terminal region of the RNA of the El Amar strain of plum pox potyvirus. Journal of General Virology 72:1741–1746
    [Google Scholar]
  43. Wisler G. C., Purcifull D. E., Hiebert E. 1995; Characterization of the P1 protein and coding region of the zucchini yellow mosaic virus. Journal of General Virology 76:37–45
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-6-1253
Loading
/content/journal/jgv/10.1099/0022-1317-78-6-1253
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error