1887

Abstract

The complete genome sequence of tobacco necrosis virus strain D (Hungarian isolate, TNV-D) was determined. The genome (3762 nt) has an organization identical to that reported for TNV-D. Highly infectious synthetic transcripts from a full- length TNV-D cDNA clone were prepared, the first infectious necrovirus transcript reported. This clone was used for reverse genetic studies to map the viral genes required for replication and movement. Protoplast inoculation with Δ22 and Δ82 mutants revealed that both the 22 kDa and 82 kDa gene products are required for RNA replication. Although the products of three small central genes (p7, p7a and p7b) were not essential for RNA replication in protoplasts, mutations in these ORFs prevented infection of plants. In contrast, viral RNA accumulation and cell-to-cell movement were observed in the inoculated, but not the systemically infected, leaves of challenged with RNA lacking the intact coat protein (CP) gene. These results strongly suggest that p7, p7a, p7b and CP are involved in TNV-Dcell-to-cell and longdistance movement, respectively.

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1997-06-01
2024-04-23
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References

  1. Andriessen M., Meulewaeter F., Cornelissen M. 1995; Expression of tobacco necrosis virus open reading frame 1 and 2 is sufficient for the replication of satellite tobacco necrosis virus. Virology 212:222–224
    [Google Scholar]
  2. Argos P. 1988; A sequence motif in many polymerases. Nucleic Acids Research 16:9909–9916
    [Google Scholar]
  3. Carrington J. C., Heaton L. A., Zuidema D., Hillman B. I., Morris T. J. 1989; The genome structure of turnip crinkle virus. Virology 170:219–226
    [Google Scholar]
  4. Coutts R. H. A., Rigden J. E., Slabas A. R., Lomonossoff G. P., Wise P. J. 1991; The complete nucleotide sequence of tobacco necrosis virus strain D. Journal of General Virology 72:1521–1529
    [Google Scholar]
  5. Dalmay T., Rubino L., Burgyán J., Russo M. 1992; Replication and movement of a coat protein mutant of cymbidium ringspot tombusvirus. Molecular Plant-Microbe Interactions 5:379–383
    [Google Scholar]
  6. Dalmay T., Russo M., Burgyán J. 1993a; Repair in vivo of altered 3′ terminus of cymbidium ringspot tombusvirus RNA. Virology 192:551–555
    [Google Scholar]
  7. Dalmay T., Rubino L., Burgyán J., Kollár A., Russo M. 1993b; Functional analysis of cymbidium ringspot virus genome. Virology 194:697–704
    [Google Scholar]
  8. Drouzas A. D., Offei S. K., Coutts R. H. A. 1996; Subcellular location and expression of tobacco necrosis Necrovirus p7a protein. Journal of Phytopathology 144:297–299
    [Google Scholar]
  9. Dunn J. J., Studier F. W. 1983; Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements. Journal of Molecular Biology 166:477–485
    [Google Scholar]
  10. Gallitelli D., Hull R., Koenig R. 1985; Relationship among viruses in the tombusvirus group: nucleic acid hybridization studies. Journal of General Virology 66:1523–1531
    [Google Scholar]
  11. Grieco F., Savino V., Martelli G. P. 1996; Nucleotide sequence of the genome of a citrus isolate of olive latent virus. Archives of Virology 141:825–838
    [Google Scholar]
  12. Hacker D. L., Petty I. T. D., Wei N., Morris T. J. 1992; Turnip crinkle virus genes required for RNA replication and virus movement. Virology 186:1–8
    [Google Scholar]
  13. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Proceedings of the National Academy of Sciences, USA 82:488–492
    [Google Scholar]
  14. Lot H., Rubino L., Delecolle B., Jacquemond M., Turturo C., Russo M. 1996; Characterization, nucleotide sequence and genome organization of leek white stripe virus, a putative new species of the genus Necrovirus. Archives of Virology 141:2375–2386
    [Google Scholar]
  15. Martelli G. P., Yilmaz M. A., Savino V., Baloglu S., Grieco F., Güldür M. E., Greco N., Lafortezza R. 1996; Properties of a citrus isolate of olive latent virus 1, a new Necrovirus. European Journal of Plant Pathology 102:527–536
    [Google Scholar]
  16. Meulewaeter F., Seurinck J., Van Emmelo J. 1990; Genome structure of tobacco necrosis virus strain A. Virology 177:699–709
    [Google Scholar]
  17. Meulewaeter F., Cornelissen M., Van Emmelo J. 1992; Subgenomic RNAs mediated expression of cistrons located internally on the genomic RNA of tobacco necrosis virus strain A. Journal of Virology 66:6419–6428
    [Google Scholar]
  18. Mushegian A. R., Koonin E. V. 1993; Cell-to-cell movement of plant viruses. Insights from amino acid sequence comparison of movement proteins and from analogies with cellular transport system. Archives of Virology 133:239–257
    [Google Scholar]
  19. Offei S. K., Coutts R. H. A. 1996; Location of the 5′ termini of tobacco necrosis virus strain D subgenomic mRNAs. Journal of Phytopathology 144:13–17
    [Google Scholar]
  20. Offei S. K., Coffin R. S., Coutts R. H. A. 1995; The tobacco necrosis virus p7a protein is a nucleic acid-binding protein. Journal of General Virology 76:1493–1496
    [Google Scholar]
  21. Russo M., Burgyán J., Martelli G. P. 1994; The molecular biology of Tombusviridae. Advances in Virus Research 44:321–428
    [Google Scholar]
  22. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  23. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 74:5463–5467
    [Google Scholar]
  24. White J. L., Kaper J. M. 1989; A simple method for detection of viral satellite RNAs in small tissue samples. Journal of Virological Methods 23:83–94
    [Google Scholar]
  25. White K. A., Skuzeski J. M., Li W., Wei N., Morris T. J. 1995; Immunodetection, expression strategy and complementation of turnip crinkle virus p28 and replication components. Virology 211:525–534
    [Google Scholar]
  26. Zhang L., French R., Langenberg W. G. 1993; Molecular cloning and sequencing of the coat protein gene of Nebraska isolate of tobacco necrosis virus : the deduced coat protein sequence has only moderate homology with those of strain A and strain D. Archives of Virology 132:291–305
    [Google Scholar]
  27. Zimmern D., Kaesberg P. 1978; 5′-Terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcription and chain-terminating inhibitors. Proceedings of the National Academy of Sciences, USA 75:4257–4261
    [Google Scholar]
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