1887

Abstract

Mutational analysis of defective interfering (DI) RNAs of Cymbidium ringspot virus (CymRSV) was used to study the mechanism of DI RNA evolution. It was shown that a highly base-paired structure in the 3′ region of the longer DI RNA directed the formation of smaller DI RNA molecules. Mutations which increased the stability of the computer- predicted, highly structured 3′ region of the longest DI RNA of CymRSV significantly enhanced the generation and accumulation of the smaller derivatives. Sequence analysis of smaller progeny molecules revealed that the highly base-paired region was deleted from the precursor DI RNA. Moreover, sites of recombination were found in other regions of the DI RNA progenies due to transposition of the highly base-paired structure. It is likely that the deletion event was structure- and not sequence- specific, and operated when a foreign sequence containing a 37-nt-long base-paired stem was inserted at the appropriate position of DI RNA.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-6-1227
1997-06-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/6/9191912.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-6-1227&mimeType=html&fmt=ahah

References

  1. Burgyán J., Grieco F., Russo M. 1989; A defective interfering RNA molecule in cymbidium ringspot virus infections. Journal of General Virology 70:235–239
    [Google Scholar]
  2. Burgyán J., Rubino L., Russo M. 1991; De novo generation of cymbidium ringspot virus defective interfering RNA. Journal of General Virology 72:505–509
    [Google Scholar]
  3. Burgyán J., Dalmay T., Rubino L., Russo M. 1992; The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules. Virology 190:579–586
    [Google Scholar]
  4. Carpenter C. D., Oh J. W., Zhang C., Simon A. E. 1995; Involvement of a stem-loop structure in the location of junction sites in viral RNA recombination. Journal of Molecular Biology 245:608–622
    [Google Scholar]
  5. Cascone P. J., Haydar T., Simon A. E. 1993; Sequences and structures required for recombination between virus associated RNAs. Science 290:801–805
    [Google Scholar]
  6. Dalmay T., Rubino L., Burgyán J., Kollár A., Russo M. 1993; Functional analysis of cymbidium ringspot virus genome. Virology 194:697–704
    [Google Scholar]
  7. Dalmay T., Szittya G. Y., Burgyán J. 1995; Generation of defective interfering RNA dimer of cymbidium ringspot tombusvirus. Virology 207:510–517
    [Google Scholar]
  8. Finnen R. L., Rochon D. M. 1993; Sequence and structure of defective interfering RNAs associated with cucumber necrosis virus infections. Journal of General Virology 74:1715–1720
    [Google Scholar]
  9. Havelda Z., Dalmay T., Burgyán J. 1995; Localization of cis-acting sequences essential for cymbidium ringspot tombusvirus defective interfering RNA replication. Journal of General Virology 76:2311–2316
    [Google Scholar]
  10. Kollár A., Burgyán J. 1994; Evidence that ORF 1 and 2 are the only virus encoded replicase genes of cymbidium ringspot tombusvirus. Virology 201:169–172
    [Google Scholar]
  11. Koonin E., Dolja V. V. 1993; Evolution and taxonomy of positive- strand viruses: implications of comparative amino acid sequences. Critical Reviews in Biochemistry and Molecular Biology 28:375–430
    [Google Scholar]
  12. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Proceedings of the National Academy of Sciences, USA 82:488–492
    [Google Scholar]
  13. Nagy P. D., Bujarski J. J. 1993; Targeting the site of RNA-RNA recombination in brome mosaic virus with antisense sequences. Proceedings of the National Academy of Sciences, USA 90:6390–6394
    [Google Scholar]
  14. Roux L., Simon A. E., Holland J. J. 1991; Effects of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo. Advances in Virus Research 40:181–211
    [Google Scholar]
  15. Russo M., Burgyán J., Martelli G. P. 1994; The molecular biology of Tombusviridae. Advances in Virus Research 44:321–428
    [Google Scholar]
  16. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Simon A. E., Bujarski J. J. 1994; RNA-RNA recombination and evolution in virus-infected plants. Annual Review of Phytopathology 32:337–362
    [Google Scholar]
  18. Thole V., Dalmay T., Burgyán J., Balázs E. 1993; Cloning and sequencing of potato virus Y (Hungarian isolate) genomic RNA. Gene 123:149–156
    [Google Scholar]
  19. White K. A., Morris T. J. 1994a; Nonhomologous RNA recombination in tombusviruses : generation and evolution of defective interfering RNAs by stepwise deletions. Journal of Virology 68:14–24
    [Google Scholar]
  20. White K. A., Morris T. J. 1994b; Enhanced competitiveness of tomato bushy stunt virus defective interfering RNAs by segment duplications or nucleotide insertion. Journal of Virology 68:6092–6096
    [Google Scholar]
  21. White K. A., Morris T. J. 1995; RNA determinants of junction site selection in RNA virus recombinants and defective interfering RNAs. RNA 1:1029–1040
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-6-1227
Loading
/content/journal/jgv/10.1099/0022-1317-78-6-1227
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error