1887

Abstract

Pothos latent virus (PoLV) is an isometric virus with a positive-sense, single-stranded RNA genome of 4415 nt. The genome contains five open reading frames (ORF), coding for five proteins with approximate molecular masses of 25, 84, 40, 27 and 14 kDa, respectively. synthesized PoLV RNA was infectious to plants and protoplasts, but could not support replication of the defective interfering (DI) and satellite RNAs associated with Cymbidium ringspot tombusvirus. No DI RNA related to PoLV was generated after repeated passaging with infected sap. Mutagenesis studiesdefined the role of the ORF 4 product (27 kDa) as a movement protein, and the ORF 5 product (14 kDa) as being responsible for symptom severity. Moreover, it was shown that the coat protein (CP) is important in regulating synthesis of the 14 kDa protein, excess production of which is lethal to infected plants. CP mutants defective in capsid formation infected plants systemically and induced severe necrotic symptoms. Conversely, CP mutants able to form apparently normal virus particles induced symptoms indistinguishable from those elicited by wild-type virus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-6-1219
1997-06-01
2022-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/6/9191911.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-6-1219&mimeType=html&fmt=ahah

References

  1. Burgyan J., Rubino L., Russo M. 1991; De novo generation of Cymbidium ringspot virus defective interfering RNA. Journal of General Virology 72:505–509
    [Google Scholar]
  2. Burgyan J., Dalmay T., Rubino L., Russo M. 1992; The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules. Virology 190:579–586
    [Google Scholar]
  3. Dalmay T., Rubino L., Burgyan J., Kollar A., Russo M. 1993; Functional analysis of cymbidium ringspot virus genome. Virology 194:697–704
    [Google Scholar]
  4. Dunn J. J., Studier F. W. 1983; Complete nucleotide sequence of bacteriophage T7 DNA and the location of T7 genetic elements. Journal of Molecular Biology 166:477–535
    [Google Scholar]
  5. Gallitelli D., Hull R. 1985; Characterization of satellite RNAs associated with tomato bushy stunt virus and five other definitive tombusviruses. Journal of General Virology 66:1533–1543
    [Google Scholar]
  6. Havelda Z., Burgyan J. 1995; 3′-terminal putative stem-loop structure required for the accumulation of cymbidium ringspot viral RNA. Virology 214:269–272
    [Google Scholar]
  7. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Proceedings of the National Academy of Sciences, USA 82:488–492
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
    [Google Scholar]
  9. Mushegian A. R., Koonin E. V. 1993; Cell-to-cell movement of plant viruses. Archives of Virology 133:239–257
    [Google Scholar]
  10. Restrepo M. A., Freed D. D., Carrington J. C. 1990; Nuclear transport of plant potyviral proteins. Plant Cell 2:987–998
    [Google Scholar]
  11. Rochon D. M., Johnston J. C., Riviere C. J. 1991; Molecular analysis of the cucumber necrosis virus genome. Canadian Journal of Plant Pathology 13:142–154
    [Google Scholar]
  12. Rubino L., Burgyan J., Grieco F., Russo M. 1990; Sequence analysis of cymbidium ringspot virus satellite and defective interfering RNAs. Journal of General Virology 71:1655–1660
    [Google Scholar]
  13. Rubino L., Carrington J. C., Russo M. 1992; Biologically active cymbidium ringspot virus satellite RNA in transgenic plants suppresses accumulation of DI RNA. Virology 188:429–437
    [Google Scholar]
  14. Rubino L., Russo M., Martelli G. P. 1995a; Sequence analysis of pothos latent virus genomic RNA. Journal of General Virology 76:2835–2839
    [Google Scholar]
  15. Rubino L., Burgyan J., Russo M. 1995b; Molecular cloning and complete nucleotide sequence of carnation Italian ringspot tombusvirus genomic and defective interfering RNAs. Archives of Virology 140:2027–2039
    [Google Scholar]
  16. Russo M., Burgyan J., Martelli G. P. 1994; The molecular biology of Tombusviridae. Advances in Virus Research 44:381–428
    [Google Scholar]
  17. Sabanadzovic S., Boscia D., Saldarelli P., Martelli G. P., Lafortezza R., Koenig R. 1995; Characterization of pothos (Scindapsus aureus) virus with unusual properties. European Journal of Plant Pathology 101:171–182
    [Google Scholar]
  18. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  19. Scholthof H. B., Morris T. J., Jackson A. O. 1993; The capsid protein gene of tomato bushy stunt virus is dispensable for systemic movement and can be replaced for localized expression of foreign genes. Molecular Plant-Microbe Interactions 6:309–322
    [Google Scholar]
  20. Scholthof H. B., Scholthof K.-B. G., Jackson A. O. 1995a; Identification of tomato bushy stunt virus host-specific symptom determinants by expression of individual genes from a potato virus X vector. Plant Cell 7:1157–1172
    [Google Scholar]
  21. Scholthof K.-B. G., Scholthof H. B., Jackson A. O. 1995b; The effect of defective interfering RNAs on the accumulation oftomato bushy stunt virus proteins and implications for disease attenuation. Virology 211:324–328
    [Google Scholar]
  22. White K. A., Morris T. J. 1994; Recombination between defective tombusvirus RNAs generates functional hybrid genomes. Proceedings of the National Academy of Sciences, USA 91:3642–3646
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-6-1219
Loading
/content/journal/jgv/10.1099/0022-1317-78-6-1219
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error