1887

Abstract

Two simian immunodeficiency virus strain mac (SIVmac)/human immunodeficiency virus type 1(HIV-1) chimeric viruses (SHIVs), designated NM-3 and NM-3n, with derived from HIV-1 and defective (plus defective for NM-3), were inoculated into seven macaques. These macaques were transiently or persistently infected and most of them produced long-lasting neutralizing antibodies and Env-specific killer T cells to HIV-1 with no AIDS-like symptoms. When they were challenged with another SHIV with intact and (designated NM-3rN), all were protected as judged by virus recovery, DNA detection by PCR and antibody responses. Anti-HIV-1 Env-specific killer T cells were considered to have played a major role in this protection, but a non-specific defence mechanism as well as specific immunity also appeared to be involved. Thus, these two non-pathogenic SHIVs induced long-lasting protective immunities in macaques, suggesting the possibility of gene- defective SHIVs as attenuated live vaccines for human use.

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/content/journal/jgv/10.1099/0022-1317-78-5-985
1997-05-01
2024-04-19
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References

  1. Almond N., Kent K., Cranage M., Rud E., Clarke B., Stott E. J. 1995; Protection by attenuated simian immunodeficiency virus in macaques against challenge with virus-infected cells. Lancet 345:1342–1344
    [Google Scholar]
  2. Baba T. W., Jeong Y. S., Penninck D., Bronson R., Greene M. F., Ruprecht R. M. 1995; Pathogenicity of live, attenuated SIV after mucosal infection of neonatal macaques. Science 267:1820–1825
    [Google Scholar]
  3. Clements J. E., Montelaro R. C., Zink M. C., Amedee A. M., Miller S., Trichel A. M., Jagerski B., Hauer D., Martin L. N., Bohm R. P., Murphy-Corb M. 1995; Cross-protective immune responses induced in rhesus macaques by immunization with attenuated macrophage-tropic simian immunodeficiency virus. Journal of Virology 69:2737–2744
    [Google Scholar]
  4. Daniel M. D., Kirchhoff F., Czajak S. C., Sehgal P. K., Desrosiers R. C. 1992; Protective effects of a live attenuated SIV vaccine with a deletion in the nef gene. Science 258:1938–1941
    [Google Scholar]
  5. Igarashi T., Shibata R., Hasebe F., Ami Y., Shinohara K., Komatsu T., Stahl-Hennig C., Petry H., Hunsmann G., Kuwata T., Jin M., Adachi A., Kurimura T., Okada M., Miura T., Hayami M. 1994; Persistent infection with SIVmac chimeric virus having tat, rev, vpu, env and nef of HIV-1 in macaque monkeys. AIDS Research and Human Retroviruses 10:1021–1029
    [Google Scholar]
  6. Igarashi T., Kuwata T., Takehisa J., Ibuki K., Shibata R., Mukai R., Komatsu T., Adachi A., Ido E., Hayami M. 1996; Genomic and biological alteration of a human immunodeficiency virus type 1 (HIV-1)-simian immunodeficiency virus strain mac chimera, with HIV-1 Env, recovered from a long-term carrier monkey. Journal of General Virology 77:1649–1658
    [Google Scholar]
  7. Kestler H. W., Ringler D. J., Mori K., Panicali D. L., Sehgal P. K., Daniel M. D., Desrosiers R. C. 1991; Importance of the nef gene for maintenance of high virus loads and for development of AIDS. Cell 65:651–662
    [Google Scholar]
  8. Kuwata T., Igarashi T., Ido E., Jin M., Mizuno A., Chen J., Hayami M. 1995; Construction of human immunodeficiency virus 1/simian immunodeficiency virus strain mac chimeric viruses having vpr and/or nef of different parental origins and their in vitro and in vivo replicaton. Journal of General Virology 76:2181–2191
    [Google Scholar]
  9. Lang S. M., Weeger M., Stahl-Hennig C., Coulibaly C., Hunsmann G., Müller J., Müller-Hermelink H., Fuchs D., Wachter H., Daniel M. M., Desrosiers R. C., Fleckenstein B. 1993; Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus. Journal of Virology 67:902–912
    [Google Scholar]
  10. Norley S., Beer B., Binninger-Schinzel D., Cosma C., Kurth R. 1996; Protection from pathogenic SIVmac challenge following shortterm infection with a nef-deficient attenuated virus. Virology 219:195–205
    [Google Scholar]
  11. Shibata R., Kawamura M., Sakai H., Hayami M., Ishimoto A., Adachi A. 1991; Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells. Journal of Virology 65:3514–3520
    [Google Scholar]
  12. Shibata R., Sakai H., Kawamura M., Tokunaga K., Adachi A. 1995; Early replication block of human immunodeficiency virus type 1 in monkey cells. Journal of General Virology 76:2723–2730
    [Google Scholar]
  13. Wyand M. S., Manson K. H., Garcia-Moll M., Montefiori D., Desrosiers R. C. 1996; Vaccine protection by a triple deletion mutant of simian immunodeficiency virus. Journal of Virology 70:3724–3733
    [Google Scholar]
  14. Yamamoto H., Miller M. D., Tsubota H., Watkins D. I., Mazzara G. P., Stallard V., Panicali D. L., Aldovini A., Young R. A., Letvin N. L. 1990; Studies of cloned simian immunodeficiency virus-specific T lymphocytes. Journal of Immunology 44:3385–3391
    [Google Scholar]
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