1887

Abstract

Intranasal inoculation of BALB/c mice with a recent clinical isolate of equine herpesvirus 2 (EHV-2 strain MR) produced a productive infection characterized by clinical signs, including weight-loss and conjunctivitis, but no mortality. Infectious virus was isolated from the lung, trachea and nasal turbinates with the highest titres present in lung tissue; EHV-2 neutralizing antibody was detected in the serum on day 21 post-inoculation. No infectious virus was detected in neural tissue, blood or lymphoid tissues. An EHV-2-specific nested PCR confirmed the presence of EHV-2 DNA in the respiratory tissues. In addition, EHV-2 DNA was detected in the trigeminal ganglia and olfactory bulbs from the first day after inoculation and these tissues remained positive for EHV-2 DNA for at least 30 days, by which time EHV- 2 DNA had been cleared from all other tissues.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-5-1119
1997-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/5/9152432.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-5-1119&mimeType=html&fmt=ahah

References

  1. Agius C. T., Studdert M. J. 1994; Equine herpesviruses 2 and 5: comparisons with other members of the subfamily gammaherpesvirinae. Advances in Virus Research 44:357–379
    [Google Scholar]
  2. Azmi M., Field H. J. 1993; Interactions between equine herpesvirus type 1 and equine herpesvirus type 4 : T cell responses in a murine infection model. Journal of General Virology 74:2339–2345
    [Google Scholar]
  3. Bagust T. J., Pascoe R. R., Harden T. J. 1972; Studies on equine herpesviruses 3. The incidence in Queensland of three different equine herpesvirus infections. Australian Veterinary Journal 48:47–53
    [Google Scholar]
  4. Blakeslee J. R. Jr Olsen R. G., McAllister E. S., Fassbender J., Dennis R. 1975; Evidence of respiratory tract infection induced by equine herpesvirus, type 2, in the horse. Canadian Journal of Microbiology 21:1940–1946
    [Google Scholar]
  5. Browning G. F., Studdert M. J. 1987; Genomic heterogeneity of equine betaherpesviruses. Journal of General Virology 68:1441–1447
    [Google Scholar]
  6. Browning G. F., Studdert M. J. 1988; Equine herpesvirus 2 (equine cytomegalovirus). Veterinary Bulletin 58:775–790
    [Google Scholar]
  7. Collinson P. N., O’Rielly J. L., Ficorilli N., Studdert M. J. 1994; Isolation of equine herpesvirus type 2 (equine gammaherpesvirus 2) from foals with keratoconjunctivitis. Journal of the American Veterinary Medical Association 205:329–331
    [Google Scholar]
  8. Edington N., Welch H. M., Griffiths L. 1994; The prevalence of latent equid herpesviruses in the tissues of 40 abattoir horses. Equine Veterinary Journal 26:140–142
    [Google Scholar]
  9. Erasmus B. J. 1970; Equine cytomegaloviruses. In Equine Infectious Diseases II, Proceedings of the 2nd International Conference on Equine Infectious Diseases pp. 46–55 Bryans J. T., Gerber H. Edited by Basel: Karger;
    [Google Scholar]
  10. Genetics Computer Group 1991; Program Manual for the GCG Package Version 7, April 1991.
  11. Gleeson L. J., Coggins L. 1985; Equine herpesvirus type 2: cell-virus relationship during persistent cell-associated viremia. American Journal of Veterinary Research 46:19–23
    [Google Scholar]
  12. Gleeson L. J., Studdert M. J. 1977; Equine herpesviruses. Experimental infection of a foetus with type 2. Australian Veterinary Journal 53:360–362
    [Google Scholar]
  13. Jolly P. D., Fu Z. F., Robinson A. J. 1986; Viruses associated with respiratory disease of horses in New Zealand: an update. New Zealand Veterinary Journal 34:46–50
    [Google Scholar]
  14. Karpas A. 1966; Characterisation of a new herpes-like virus isolated from foal kidney. Annales de l’Institut Pasteur 110:688–696
    [Google Scholar]
  15. Kitchin P. A., Almond N. M., Szotyori Z., Fromholc C., McAlpine L., Silvera P., Stott E. J., Cranage M. P., Baskerville A., Schild G. C. 1990; The use of the polymerase chain reaction for the detection of simian immunodeficiency virus in experimentally infected macaques. Journal of Virological Methods 28:85–100
    [Google Scholar]
  16. Plummer G., Waterson A. P. 1963; Equine herpesviruses. Virology 19:412–416
    [Google Scholar]
  17. Plummer G., Coleman P. L., Henson D. 1972; Chronic infection of the rabbit central nervous system by a slowly growing equine herpesvirus. Infection and Immunity 5:172–175
    [Google Scholar]
  18. Rizvi S. M., Slater J. D., Wolfinger U., Borchers K., Field H. J., Slade A. J. 1997; Detection and distribution of equine herpesvirus 2 DNA in the central and peripheral nervous systems of ponies. Journal of General Virology 78:1115–1118
    [Google Scholar]
  19. Roeder P. L., Scott G. R. 1975; The prevalence of equid herpes virus 2 infections. Veterinary Record 96:404–405
    [Google Scholar]
  20. Roizman B., Desrosiers R. C., Fleckenstein B., Lopez C., Minson A. C., Studdert M. J. 1992; The family herpesviridae: an update. Archives of Virology 123:425–449
    [Google Scholar]
  21. Rose M. A., Hopes R., Rossdale P. D., Beveridge W. I. B. 1974; Virus infections of horses at Newmarket, 1972 and 1973. Veterinary Record 95:484–488
    [Google Scholar]
  22. Slater J. D., Borchers K., Thackray A. M., Field H. J. 1994; The trigeminal ganglion is a location for equine herpesvirus 1 latency and reactivation in the horse. Journal of General Virology 75:2007–2016
    [Google Scholar]
  23. Studdert M. J. 1971; Equine herpesviruses 4. Concurrent infection in horses with strangles and conjunctivitis. Australian Veterinary Journal 47:434–436
    [Google Scholar]
  24. Sugiura T., Fukuzawa Y., Kamada M., Ando Y., Hirasawa K. 1983; Isolation of equine herpesvirus type 2 from foals with pneumonitis. Bulletin of the Equine Research Institute 20:148–153
    [Google Scholar]
  25. Telford E. A. R., Watson M. S., McBride K., Davison A. J. 1992; The DNA sequence of equine herpesvirus 1. Virology 189:304–316
    [Google Scholar]
  26. Telford E. A. R., Studdert M. J., Agius C. T., Watson M. S., Aird H. C., Davison A. J. 1993; Equine herpesviruses 2 and 5 are gamma herpesviruses. Virology 195:492–499
    [Google Scholar]
  27. Telford E. A. R., Watson M. S., Aird H. C., Perry J., Davison A. J. 1995; The DNA sequence of equine herpesvirus 2. Journal of Molecular Biology 249:520–528
    [Google Scholar]
  28. Welch H. M., Bridges C. G., Lyon A. M., Griffiths L., Smith F., Edington N. 1992; Latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and co-cultivation from lymphoid tissues. Journal of General Virology 73:261–268
    [Google Scholar]
  29. Wilks C. R., Studdert M. J. 1976; Equine herpesviruses 6. Sequential infection of horses with type 2, 3 and 1. Australian Veterinary Journal 52:199–203
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-5-1119
Loading
/content/journal/jgv/10.1099/0022-1317-78-5-1119
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error