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125I protein labelling of oligo(dT)-selected RNAfrom feline calicivirus (FCV)-infected cells revealed that the genomic and 2·4 kb subgenomic RNAs of FCV are linked to a 15 kDa protein (VPg). Proteinase K treatment of FCV RNA, to remove VPg, led to a decrease in the translatability of the RNA, but there was no obvious change in the site of RNA initiation. Addition of the cap analogue 7-methylGTP to in vitro translations had no effect on the translation of FCV RNA, suggesting that FCV RNA is translated by a cap-independent mechanism. Further evidence that FCV RNA is translated by an unusual mechanism was obtained by translating FCV RNA in vitro at a range of K concentrations. FCV RNA was able to direct translation at K concentrations at which cellular RNA translation was inhibited.
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