1887

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Volume 78, Issue 5

Identification of a protein linked to the genomic and subgenomic mRNAs of feline calicivirus and its role in translation. Free

Abstract

I protein labelling of oligo(dT)-selected RNAfrom feline calicivirus (FCV)-infected cells revealed that the genomic and 2·4 kb subgenomic RNAs of FCV are linked to a 15 kDa protein (VPg). Proteinase K treatment of FCV RNA, to remove VPg, led to a decrease in the translatability of the RNA, but there was no obvious change in the site of RNA initiation. Addition of the cap analogue 7-methylGTP to translations had no effect on the translation of FCV RNA, suggesting that FCV RNA is translated by a cap-independent mechanism. Further evidence that FCV RNA is translated by an unusual mechanism was obtained by translating FCV RNA at a range of K concentrations. FCV RNA was able to direct translation at K concentrations at which cellular RNA translation was inhibited.

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© Society for General Microbiology Ltd 1997
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1997-05-01
2024-04-25
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Identification of a protein linked to the genomic and subgenomic mRNAs of feline calicivirus and its role in translation.
J. Gen. Virol. 78, 1033 (1997); https://doi.org/10.1099/0022-1317-78-5-1033
/content/journal/jgv/10.1099/0022-1317-78-5-1033
/content/journal/jgv/10.1099/0022-1317-78-5-1033
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