1887

Abstract

A cloned strain of granulovirus, CpGV-M1, was obtained using successive rounds of an limiting dilution method. A detailed physical map of the genome was constructed using 11 restriction enzymes. The region containing the granulin gene and an open reading frame immediately upstream of the granulin gene was sequenced. This region showed a high degree of homology to the equivalent region from granulovirus with 98% amino acid identity for the granulins and 68% identity for the putative polypeptides encoded by the upstream ORFs. These latter polypeptides contained two zinc finger-like motifs and showed a low degree of homology to ME53 from nucleopoly- hedrovirus (AcMNPV). Evidence is presented for a similar upstream ORF in GV also. Hybridization studies showed that the CpGV genome had a similar overall organization to the GV genome. Hybridization between CpGV and AcMNPV was limited to fragments spanning about 15% of each genome suggesting that very few genes are highly conserved between GVs and NPVs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-78-4-965
1997-04-01
2022-05-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/78/4/9129672.html?itemId=/content/journal/jgv/10.1099/0022-1317-78-4-965&mimeType=html&fmt=ahah

References

  1. Akiyoshi D., Chakerian R., Rohrmann G. F., Nesson M. H., Beaudreau G. S. 1985; Cloning and sequencing of the granulin gene from the Trichoplusia ni granulosis virus. Virology 141:328–332
    [Google Scholar]
  2. Ayres M. D., Howard S. C., Kuzio J., Lopez-Ferber M., Possee R. D. 1994; The complete DNA sequence of Autographa californica nuclear polyhedrosis virus. Virology 202:586–605
    [Google Scholar]
  3. Chakerian R., Rohrmann G. F., Nesson M. H., Leisy D. J., Beaudreau G. S. 1985; The nucleotide sequence of the Pieris brassicae granulosis virus granulin gene. Journal of General Virology 66:1263–1269
    [Google Scholar]
  4. Crook N. E. 1986; Restriction enzyme analysis of granulosis viruses isolated from Artogeia rapae and Pieris brassicae. Journal of General Virology 67:781–787
    [Google Scholar]
  5. Crook N. E. 1991; Baculoviridae: subgroup B: comparative aspects of granulosis viruses. In Viruses of Invertebrates pp. 73–110 Kurstak E. Edited by New York: Marcel Dekker;
    [Google Scholar]
  6. Crook N. E., Clem R. J., Miller L. K. 1993; An apoptosis-inhibiting baculovirus gene with a zinc finger-like motif. Journal of Virology 67:2168–2174
    [Google Scholar]
  7. Crook N. E., Spencer R. A., Payne C. C., Leisy D. J. 1985; Variation in Cydia pomonella granulosis virus isolates and physical maps of the DNA from three variants. Journal of General Virology 66:2423–2430
    [Google Scholar]
  8. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  9. Jehle J. A., Backhaus H. 1994; The granulin gene region of Cryptophlebia leucotreta granulosis virus: sequence analysis and phylogenetic considerations. Journal of General Virology 75:3667–3671
    [Google Scholar]
  10. Knauf V. C., Nester E. W. 1982; Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid 8:45–54
    [Google Scholar]
  11. Knebel-Morsdorf D., Kremer A., Jahnel F. 1993; Baculovirus gene ME53, which contains a putative zinc finger motif, is one of the major early-transcribed genes. Journal of Virology 67:753–758
    [Google Scholar]
  12. Maeda S. 1996; Bombyx mori nuclear polyhedrosis virus (individual isolate T3) DNA, complete genome. GenBank accession no. L33180
    [Google Scholar]
  13. Rohrmann G. F. 1986; Polyhedrin structure. Journal of General Virology 67:1499–1513
    [Google Scholar]
  14. Rohrmann G. F., Funk C. J., Ahrens C., Russell R. 1996; Characterization of the genome of a baculovirus pathogenic for Orgyia pseudotsugata. Abstracts of the 15th Annual Meeting of the American Society for Virology p. 105 London, Ontario:
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Schaffer H. E., Sederoff R. R. 1981; Improved estimation of DNA fragment lengths from agarose gels. Analytical Biochemistry 115:113–122
    [Google Scholar]
  17. Smith I. R. L. 1994; Characterization of the Lacanobia oleracea granulosis virus granulin gene. Abstracts of the VIth International Colloquium on Invertebrate Pathology and Microbial Control p. 152 Montpellier, France:
    [Google Scholar]
  18. Smith I. R. L., Crook N. E. 1988a; In vivo isolation of baculovirus genotypes. Virology 166:240–244
    [Google Scholar]
  19. Smith I. R. L., Crook N. E. 1988b; Physical maps of the genomes of four variants of Artogeia rapae granulosis virus. Journal of General Virology 69:1741–1747
    [Google Scholar]
  20. Winstanley D., Crook N. E. 1993; Replication of Cydia pomonella granulosis virus in cell cultures. Journal of General Virology 74:1599–1609
    [Google Scholar]
  21. Zanotto P. M. A., Kessing B. D., Maruniak J. E. 1993; Phylogenetic interrelationships among baculoviruses: evolutionary rates and host associations. Journal of Invertebrate Pathology 62:147–164
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-78-4-965
Loading
/content/journal/jgv/10.1099/0022-1317-78-4-965
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error